Article
Food Science & Technology
Xinlu Wang, Linxia Wu, Jing Qiu, Yongzhong Qian, Meng Wang
Summary: In this study, non-targeted metabolomics analysis was conducted on 10 strawberry species using UHPLC-Q-Exactive Orbitrap MS. A total of 142 compounds were identified and classified into six categories. Screening 72 differential metabolites revealed that Tochiotome differed the most from the white strawberry (Baiyu). Histidine, apigenin, cyanidin 3-glucoside, and peonidin 3-glucoside were identified as potential biomarkers for distinguishing Baiyu and other strawberry groups. Amino acid metabolism, anthocyanin biosynthesis, and flavonoid biosynthesis pathways played a major role in determining the nutritional distinctions. This research contributes to the determination of the nutrition and health benefits of different strawberry species.
Article
Chemistry, Multidisciplinary
Pei Xiong, Shi-han Qin, Kai-lin Li, Ming-juan Liu, Lian Zhu, Jie Peng, Si-lin Shi, Su-nv Tang, Ai-ping Tian, Wei Cai
Summary: This study established a method based on UHPLC-Q-Exactive Orbitrap MS to identify tannins in Paeoniae Radix Alba (PRA), identifying a total of 106 constituents with 75 reported for the first time. This lays the foundation for further research on the efficacy of PRA and provides a scientific basis for selecting quality markers.
ARABIAN JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Applied
Changling Jiang, Haolei Han, Jinxia Dai, Ziqi Wang, Yunfeng Chai, Chen Wang, Xin Liu, Chengyin Lu, Hongping Chen
Summary: This study investigated the degradation behavior of gibberellic acid (GA3) under different stress factors, such as light, pH, and temperature. Five degradation products were identified, with three produced under ultraviolet photolysis conditions and two isomers formed under alkaline conditions. The results provide practical reference for the safety of agricultural products and guidance for the scientific application of GA3 and storage conditions.
Article
Biochemistry & Molecular Biology
Linlin Li, Fan Dong, Bianli Wang, Jian Song, Huimin Zhang, Ping Wang, Feiran Wang, Yingying Yan, Xiao Zhang
Summary: This study analyzed the metabolic characteristics and mechanism of neobavaisoflavone for the first time. A total of 72 metabolites were identified, and the metabolic pathways in vivo and in vitro were determined. Furthermore, active components and corresponding targets were predicted, and enriched signaling pathways were identified. These results expand the potential effects of neobavaisoflavone and provide a scientific basis for its further exploitation and application.
Article
Biochemistry & Molecular Biology
Huan Li, Lei Gao, Huili Shao, Bingqian Li, Chao Zhang, Huagang Sheng, Liqiao Zhu
Summary: This study utilized UHPLC-Q-Exactive Orbitrap MS and network pharmacology to identify the active components and mechanism of hawthorn in the prevention and treatment of atherosclerosis. The study revealed the core targets and signaling pathways involved, providing a theoretical and scientific support for the application of hawthorn and the study of other herbal medicines.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Tao Liu, Xingze Cao, Dongmin Cao
Summary: This study identified the compounds and mechanisms of action of Eucommia ulmoides leaves using LC-MS and network pharmacology. The results showed that EULs were effective in treating OA by regulating key pathways including NF-kappa B signaling pathway, PI3K-Akt signaling pathway, and AMPK signaling pathway.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Lian Zhu, Shengjun Ma, Kailin Li, Pei Xiong, Shihan Qin, Wei Cai
Summary: Arnebiae Radix, a traditional Chinese medicinal herb, has been widely used for the treatment of various skin conditions. A rapid and effective method using UHPLC-Q-Exactive Orbitrap mass spectrometry was established in this study to identify its chemical constituents. A total of 188 compounds were identified, with 114 of those being reported for the first time. This method lays the foundation for further understanding the active ingredients and quality control of Arnebiae Radix.
Article
Biochemistry & Molecular Biology
Zhe Li, Weichao Dong, Yanan Li, Xin Liu, Hong Wang, Long Dai, Jiayu Zhang, Shaoping Wang
Summary: In this study, the metabolites of genistin were identified in a hyperlipidemic rat model induced by a high-fat diet. The results showed that genistin could significantly reduce lipid factors, inhibit lipid accumulation in the liver, and reverse liver function abnormalities caused by lipid peroxidation. This study provides the foundation for genistin as a new lipid-lowering agent.
Article
Food Science & Technology
Fanghua Guo, Rong Tsao, Chuyao Li, Xiaoya Wang, Hua Zhang, Li Jiang, Yong Sun, Hua Xiong
Summary: The study found that green pea hull extracts are rich in phenolic substances, such as quercetin, and have significant effects in alleviating DSS-induced colitis.
Article
Food Science & Technology
Kailin Li, LiangHong Liu, Pei Xiong, Sunv Tang, Huixin Chen, Zhenpeng Bin, Weihua Wu, Wei Cai
Summary: This study identified 57 anthocyanin derivatives from the epicarp of Kadsura coccinea, 55 of which were reported for the first time. This expands the constituent database for pharmaceutical research and quality control, while also contributing to environmental conservation efforts by reducing waste.
FOOD ANALYTICAL METHODS
(2021)
Article
Chemistry, Applied
Meiyue Dong, Zhenhua Tian, Yanni Ma, Zhongyi Yang, Zhen Ma, Xiaoming Wang, Yunlun Li, Haiqiang Jiang
Summary: This study developed an integrated data filtering and identification strategy to characterize the GSLs in 25 commonly Brassicaceae tissues, successfully identifying 47 GSLs and establishing profiles for each tissue, which can be used to distinguish different Brassicaceae species.
Article
Biochemistry & Molecular Biology
Miaomiao Chi, Kunming Qin, Lei Cao, Min Zhang, Yingying Su, Xun Gao
Summary: This study utilized magnetic ligand fishing and functionalized nano-microspheres to screen target-specific active compounds from Choerospondias axillaris. The PPAR-gamma functionalized magnetic nano-microspheres were successfully synthesized and characterized, and 16 active ligands were found in the ethanolic extracts of Choerospondias axillaris. This study provides a successful precedent for extracting and isolating target-specific active ingredient groups in complex mixtures.
Article
Biochemistry & Molecular Biology
Xinhua Zhou, Xu Chen, Liping Fan, Huirong Dong, Yan Ren, Xiangming Chen
Summary: This study developed a stepwise diagnostic product ions (DPIs) filtering strategy to efficiently and targetedly profile diterpenoids in Scutellaria barbata using UHPLC-Q-Exactive-Orbitrap-MS. The results showed that the stepwise DPIs method simplified the workload in data post-processing and enabled the identification of a large number of diterpenoids in Scutellaria barbata.
Article
Food Science & Technology
Jingxuan Li, Shiqi Zou, Wanling Yang, Mengdie Peng, Baizhong Chen, Jinji Deng, Minyan Wei, Guodong Zheng
Summary: Gas chromatograph-mass spectrometer (GC-MS), ultra-high-performance liquid chromatograph-Q-Exactive Orbitrap tandem mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS), and high-performance liquid chromatography-photodiode array detection (HPLC-PDA) were used to analyze the chemical components of Citri Reticulatae Pericarpium Viride Geqingpi (GQP). The volatile components of GQP were determined using GC-MS, and 56 volatile components were identified, with gamma-Terpinene and D-Limonene being the main terpenes. UHPLC-Q-Exactive Orbitrap-MS was used to identify nonvolatile compositions, and a total of 42 compositions were identified, including flavonoids, organic acids, coumarins, alkaloids compounds, and other compounds.
FOOD SCIENCE & NUTRITION
(2023)
Article
Pharmacology & Pharmacy
Junqi Bai, Wanting Chen, Juan Huang, He Su, Danchun Zhang, Wen Xu, Jing Zhang, Zhihai Huang, Xiaohui Qiu
Summary: This study utilized ultra high performance liquid chromatography tandem quadrupole/electrostatic field orbitrap high-resolution mass spectrometry to investigate the changes in stilbene glycosides during the processing of Reynoutria multiflora. The content and quantity of stilbene glycosides were found to undergo significant changes, with the detection of potential new compounds. The results provide a basis for further research on the pharmacodynamics and toxicity mechanisms of processed R. multiflora.
FRONTIERS IN PHARMACOLOGY
(2022)