Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume 154, Issue -, Pages 8-15Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2015.10.028
Keywords
Bone repair; LLLT; Microarray; Gene expression
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Funding
- FAPESP [2010/15335-0]
- Center for Computational Engineering and Sciences at UNICAMP SP Brazil (FAPESP/CEPID) [2013/08293-7]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [10/15335-0] Funding Source: FAPESP
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The process of bone healing as well as the expression of inflammatory and angiogenic genes after low level laser therapy (LLLT) were investigated in an experimental model of bone defects. Sixty Wistar rats were distributed into control group and laser group (830 nm, 30 mW, 2,8 J, 94 seg). Histopathological analysis showed that LLLT was able to modulate the inflammatory process in the area of the bone defect and also to produce an earlier deposition of granulation tissue and newly formed bone tissue. Microarray analysis demonstrated that LLLT produced an up-regulation of the genes related to the inflammatory process (MMD, PTGIR, PTGS2, Ptger2, ILL 1IL6, IL8, IL18) and the angiogenic genes (FGF14, FGF2, ANGPT2, ANGPT4 and PDGFD) at 36 h and 3 days, followed by the decrease of the gene expression on day 7. Immunohistochemical analysis revealed that the subjects that were treated presented a higher expression of COX-2 at 36 h after surgery and an increased VEGF expression on days 3 and 7 after surgery. Our findings indicate that LLLT was efficient on accelerating the development of newly formed bone probably by modulating the inflammatory and angiogenic gene expression as well as COX2 and VEGF immunoexpression during the initial phase of bone healing. (C) 2015 Elsevier B.V. All rights reserved.
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