Article
Acoustics
Yixuan Xu, Shiyang Liu, Hongyou Zhao, Yi Li, Chao Cui, Wenxin Chou, Yuxia Zhao, Jiyong Yang, Haixia Qiu, Jing Zeng, Defu Chen, Shengnan Wu, Yizhou Tan, Ying Wang, Ying Gu
Summary: Combining ultrasonic irradiation with antimicrobial photodynamic therapy (aPDT) enhances the efficacy of aPDT against MRSA biofilm by increasing the penetration depth of the photosensitizer (PS) and without affecting the viability of human dermal fibroblasts. This study suggests that the combination of aPDT and ultrasound is a potential and promising strategy for eradicating biofilm-associated infections of MRSA.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Jace A. Willis, Vsevolod Cheburkanov, Shaorong Chen, Jennifer M. Soares, Giulia Kassab, Kate C. Blanco, Vanderlei S. Bagnato, Paul de Figueiredo, Vladislav V. Yakovlev
Summary: The widespread use of antibiotics contributes to the evolution of antimicrobial-resistant bacteria, posing a threat to patients and healthcare professionals. Developing strategies to combat resistance is recognized as a global healthcare priority. In this study, a method using antimicrobial photodynamic therapy (aPDT) in conjunction with antibiotics is proposed to reduce resistance and extend the lifespan of current antibiotics. The results show that concurrent treatment of bacteria with antibiotics and aPDT significantly decreases the resistance of methicillin-resistant Staphylococcus aureus (MRSA) strains, suggesting a potential alternative for overcoming the challenge of growing MRSA.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Min Lu, Yongli Li, Mei X. Wu
Summary: Multidrug-resistant bacteria pose a significant threat and are depleting treatment options. This study explores the combination of blue-laser and thymol to eradicate these bacteria, successfully preventing systematic dissemination in mice. The strategy leverages the unique properties of bacteria to transform harmless thymol into a powerful sterilizer when activated by blue-laser excitation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Microbiology
Nada T. Felifel, Mahmoud A. Sliem, Zienat Kamel, Joanna Bojarska, Mohamed G. Seadawy, Rehab M. Amin, Sherif M. Elnagdy
Summary: Multidrug-resistant microorganisms pose a significant public health threat, and the use of traditional antibiotics is becoming less effective. Photodynamic therapy (PDT), which utilizes photosensitizers and light to generate Reactive Oxygen Species (ROS) for microorganism killing, shows promise as an alternative. Zinc phthalocyanine (ZnPc) is a potentially suitable photosensitizer due to its antimicrobial properties and ability to be encapsulated in nanoemulsions. In this study, a nanoemulsion was prepared using Miglyol 812N and distilled water, serving as an efficient nanocarrier system for hydrophobic drugs such as ZnPc. The results demonstrated that ZnPc encapsulated in the nanoemulsion significantly reduced cell survival percentages of Staphylococcus aureus and Escherichia coli, highlighting the potential of nanoemulsion-based PDT as an effective alternative for treating multidrug-resistant microorganisms.
Article
Oncology
Isabelle de Paula Ribeiro, Juliana Guerra Pinto, Beatriz Muller Nunes Souza, Alejandro Guillermo Minan, Juliana Ferreira-Strixino
Summary: Antimicrobial Photodynamic Therapy (aPDT) with curcumin was shown to reduce the growth of MRSA biofilm without cytotoxicity, providing a relevant alternative possibility for the inactivation of this strain.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2022)
Article
Environmental Sciences
Xiaoqiang Liu, Jingru Zuo, Jiang Teng, Le Yang, Jingjing Guo, Lianjie Liu, Pei Li
Summary: This study investigated the prevalence and multidrug-resistant (MDR) phenotype of Staphylococcus aureus in raw milk samples and farm environments. The antimicrobial and antibiofilm activities of luteolin against MDR S. aureus were also explored. The results showed that luteolin can inhibit biofilm formation, reduce cell viability, and affect gene expression related to biofilm formation in S. aureus.
ENVIRONMENTAL POLLUTION
(2023)
Article
Infectious Diseases
Sakari Nikinmaa, Anna Podonyi, Peter Raivio, Jukka Meurman, Timo Sorsa, Juha Rantala, Esko Kankuri, Tuomas Tauriainen, Tommi Paetilae
Summary: Dual-light aPDT shows improved bactericidal effect on Staphylococcus aureus biofilm compared to aPDT or aBL, providing a sustained effect. Increasing the relative ratio of aBL enhances the antibacterial effect, especially with repeated applications.
Article
Biochemistry & Molecular Biology
Lin Yue, Zhouping Wang, Meihong Zheng, Min Wang, Imran Mahmood Khan, Xiaowei Ding, Yin Zhang
Summary: In this study, Hydroxypropyl chitosan was used as a carrier of the photosensitizer chlorin e6 to develop a photodynamic antibacterial agent with good water solubility, high biocompatibility, and antibacterial activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Microbiology
Grace Kaul, Abdul Akhir, Manjulika Shukla, Hasham Shafi, Ravikumar Akunuri, Gaurav Pawar, Mahammad Ghouse, Nanduri Srinivas, Sidharth Chopra
Summary: In this study, oxiconazole, a topical antifungal agent, was repurposed as a new treatment for multidrug-resistant Staphylococcus aureus infections. It exhibited potent activity against S. aureus and showed synergistic effects with the antibiotic gentamicin, resulting in superior therapeutic outcomes.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Kannan Badri Narayanan, Gyu Tae Park, Sung Soo Han
Summary: The study identified an antagonistic compound against multidrug-resistant Staphylococcus aureus strains from Pseudomonas aeruginosa YUSA1 isolated from freshwater. The crude ethyl acetate extract showed in vitro antibacterial and antibiofilm properties against MRSA, suggesting its potential use in food packaging materials with antibacterial effects.
Article
Medicine, General & Internal
Ching Hoong Chew, Chew Chieng Yeo, Ainal Mardziah Che Hamzah, Esra'a I. Al-Trad, Sherry Usun Jones, Kek Heng Chua, Suat Moi Puah
Summary: Multidrug resistance (MDR) poses a significant challenge in healthcare management, and a comprehensive approach is needed to address this issue. In this study, a combination of phenotypic and genotypic methods, including whole genome sequencing (WGS), was used to investigate hospital-associated MDR methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from newborn infants. The findings revealed different levels of resistance to antimicrobial classes among the MDR-MRSA strains, along with the presence of slime and biofilms, SCCmec type IV, and various types of microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) and virulence genes. The WGS data for specific strains showed the presence of antimicrobial resistance genes in the chromosomes, with one gene found in a small plasmid. The presence of MDR-MRSA strains in neonates raises public concern, and multifaceted interventions are recommended to address this issue. Furthermore, there is a need for metadata to improve the investigation of antimicrobial resistance genes in MDR isolates.
Article
Biotechnology & Applied Microbiology
Jing Yan, Qi Yin, Hao Nie, Jinyou Liang, Xiang-Ru Liu, Yingli Li, Hong Xiao
Summary: Prodigiosin (PG) has been found to possess anti-biofilm activity against 29 clinical antimicrobial-resistant (AMR) Staphylococcus aureus strains. It shows synergistic effects with vancomycin (VAN) and effectively inhibits biofilm formation, reduces metabolic activity, and suppresses exopolysaccharide production. These findings suggest that PG could be used as a preventive coating or adjuvant against biofilms in clinical settings.
Article
Microbiology
Yafei Li, Wei Li, Yu Pan, Chang Liu, Siyu Liang, Zhenling Zeng
Summary: This study reveals a widespread presence of antimicrobial resistant livestock-associated MRSA (LA-MRSA) in animal farms and slaughterhouses in Chongqing, China. The MRSA isolates showed a high level of multidrug resistance, with the majority belonging to MLST type ST239 and carrying SCCmec types III, IVa, XII, and V.
VETERINARY MICROBIOLOGY
(2022)
Article
Materials Science, Biomaterials
Fei Li, Mingsong Zang, Shengda Liu, Xiumei Li, Xiaojia Jiang, Ruizhen Tian, Quan Luo, Chunxi Hou, Jiayun Xu, Junqiu Liu
Summary: The research team developed highly stable and effective polymer nanosheets that can bind with Staphylococcus aureus through electrostatic interactions and generate reactive oxygen species under light irradiation, showing satisfactory antibacterial performance. Cell viability assays demonstrated minimal to no cytotoxicity impact on mammalian cells, indicating the potential of the nanosheets as an effective antibacterial agent.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Biochemistry & Molecular Biology
Yi-Zhou Xiang, Gang Wu, Lin-Yu Yang, Xiao-Jie Yang, Yan-Mei Zhang, Lian-Bing Lin, Xian-Yu Deng, Qi-Lin Zhang
Summary: The study evaluated the antibacterial effect of bacteriocin XJS01 against multidrug-resistant Staphylococcus aureus biofilms and its potential as an antibiofilm agent. Results showed that XJS01 exhibited excellent therapeutic effect, effectively disrupting and reducing biofilms on mouse skin wounds.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Ophthalmology
Tim Berger, Berthold Seitz, Loic Hamon, Fidelis Flockerzi, Ursula Schlotzer-Schrehardt, Loay Daas
Summary: This study used light and transmission electron microscopy to examine corneal buttons after DALK surgery. The findings revealed irregularities in collagen distribution and degeneration of keratocytes in the interface area, suggesting that the choice of surgical method can affect the postoperative visual outcome.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Ophthalmology
Tim Berger, Berthold Seitz, Fidelis Flockerzi, Loay Daas
Summary: This study emphasizes the importance of using a sufficiently large corneal graft in primary penetrating keratoplasty to prevent recurrence of fungal keratitis. A clinical case of therapy-resistant fungal keratitis is analyzed, revealing that a larger graft size can effectively prevent recurrence. Therefore, in penetrating keratoplasty, optimal planning and preparation should be made to ensure an adequate safety distance and comprehensive treatment measures.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Ophthalmology
Haris Sideroudi, Elias Flockerzi, Berthold Seitz
Summary: Differentiating keratoconus from other corneal ectatic diseases is crucial for appropriate management. Various imaging techniques including corneal topography, Scheimpflug cameras, air pulse tonometer, α confocal microscopy, and AS-OCT can assist in diagnosis and differentiation.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Medicine, General & Internal
Emilia Sophie Stuhlmacher, Shady Suffo, Cristian Munteanu, Berthold Seitz, Loay Daas
Summary: This study evaluated the learning curve of individual surgeons for DMEK and Triple-DMEK surgeries and assessed the impact of experience on outcomes. The results showed a significant decrease in surgery duration, graft preparation time, and the re-bubbling rate with increased experience. However, there were no significant changes in visual acuity and central corneal thickness. Different surgeons also exhibited significant differences in surgery duration and graft preparation time.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Ophthalmology
Burak Mergen, Tarek Safi, Matthias Nadig, Gopal Bhattrai, Loay Daas, Jan Alexandersson, Berthold Seitz
Summary: The study aims to develop and evaluate an AI-based image analysis tool for measuring and quantifying corneal neovascularisation (CoNV) area. Slit lamp images of patients with CoNV were annotated by an ophthalmologist and used to create, train, and evaluate an automated tool using deep learning. The algorithm achieved high accuracy in detecting CoNV areas, demonstrating potential for AI-based calculation of CoNV area.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Ophthalmology
Kolja Berg, Tarek Safi, Berthold Seitz, Loay Daas
Summary: The aim of this study was to measure the endothelial cell density (ECD) of the in toto pre-stripped endothelial Descemet membrane lamellae (EDML) and to describe the impact of pre- and intraoperative endothelial cell loss (ECL) on postoperative midterm clinical outcome. The results showed that despite a significant decrease in ECD up to 6 months postoperatively, visual acuity further improved and thickness further decreased up to 1 year postoperatively, indicating the feasibility of non-invasive ECD measurement technique.
Article
Ophthalmology
Tim Berger, Fidelis Flockerzi, Ursula Loew, Elias Flockerzi, Wissam Aljundi, Alaadin Abdin, Loay Daas, Berthold Seitz
Summary: The purpose of this study is to report a rare and late manifestation of choroidal melanoma in the form of corneal perforation and to highlight the major histopathological findings of this unusual combined clinical presentation. The patient, a 74-year-old male, presented with corneal perforation of the right eye and absence of light perception for 6 months. Primary enucleation was performed due to the prolonged finding and reduced visual prognosis.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Ophthalmology
Zhen Li, Daniel Boehringer, Tanja Stachon, Mahsa Nastaranpour, Fabian Norbert Fries, Berthold Seitz, Myriam Ulrich, Cristian Munteanu, Achim Langenbucher, Nora Szentmary
Summary: The study assessed the impact of various factors, including donor age, storage time, and corneal ring size, on the outgrowth of human limbal epithelial cells. The results showed that lower donor age, fewer medium changes during storage, shorter medium time, and smaller corneoscleral ring size were associated with higher success rates of LEC outgrowth. A stepwise linear regression algorithm was employed to analyze the influencing parameters.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Ophthalmology
Elias Flockerzi, Christina Turner, Berthold Seitz, Geker Study Grp Collaborators, Geker Study Grp
Summary: This retrospective multicentric panel study investigated the number, types, and indications for corneal transplantation in Germany from 2011 to 2021 and compared them internationally. The study found that the reported number of keratoplasties more than doubled during this period. DMEK has become the predominant procedure since 2016, while PKP showed a slight decrease over the decade. Rating: 9/10.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Ophthalmology
Fabian Norbert Fries, Annamaria Naray, Cristian Munteanu, Tanja Stachon, Neil Lagali, Berthold Seitz, Nora Szentmary, Barbara Kaesmann-Kellner
Summary: The purpose of this study is to summarize the ophthalmic findings of aniridia subjects examined at the Department of Ophthalmology, Saarland University Medical Center. The main ophthalmic signs of congenital aniridia were found to be AAK, iris malformation, cataract, and macular hypoplasia.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Ophthalmology
Alaa Din Abdin, Anna Eppinger, Wissam Aljundi, Yaser Abu-Dail, Cristian Munteanu, Isabel Weinstein, Berthold Seitz
Summary: This study aimed to assess the vision-related quality of life of patients with age-related macular degeneration (AMD) and evaluate the differences according to different types of AMD. The results showed that all types of AMD had a negative effect on vision-related quality of life, but the effect was more pronounced in patients with exudative AMD.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Ophthalmology
Ann-Isabel Mattern, Kai Neller, Machteld Devenijn, Hartmut Schwahn, Achim Langenbucher, Berthold Seitz, Hakan Kaymak
Summary: This study assessed the reproducibility and repeatability of biometers in myopic children. The results showed good agreement between the biometers and suggested a minimum six-month interval for measuring axial growth in myopic children.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Medicine, General & Internal
Alaa Din Abdin, Machteld Devenijn, Roxana Fulga, Achim Langenbucher, Berthold Seitz, Hakan Kaymak
Summary: This study aimed to investigate the prevalence of geographic atrophy (GA) in advanced age-related macular degeneration (AMD) and the proportion of eyes eligible for treatment with intravitreal pegcetacoplan. A retrospective cross-sectional study was conducted, including all AMD patients who visited a specific center in 2021. It was found that at least a quarter of eyes with advanced AMD would meet the indication criteria for treatment with pegcetacoplan, and neovascular AMD was associated with a higher likelihood of foveal involvement in GA compared to dry AMD.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Health Care Sciences & Services
Caroline Chan, Berthold Seitz, Barbara Kaesmann-Kellner
Summary: This study investigated a large sample of achromatopsia patients and found that the disease is predominantly stable. The most commonly used visual aids are edge filter glasses and enlarged reading glasses.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Health Care Sciences & Services
Madeleine A. Perrault, Gabriele Lauer, Sabine Voss, Berthold Seitz, Barbara Kaesmann-Kellner
Summary: This study aims to compare the etiology and fitting of low vision aids in visually impaired children and adolescents with that of adults. The study found that children had better visual acuity compared to adults and the most common cause of visual impairment in children was albinism while it was age-related macular degeneration in adults. Fitting magnifying visual aids significantly improved near and distance visual acuity, with optical aids being the most commonly prescribed aids.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Girgis Obaid, Menitte Eroy, Jie Zhao, Shazia Bano, Mari Mino-Kenudson, Tayyaba Hasan
Summary: The presence of desmoplasia in pancreatic ductal adenocarcinoma (PDAC) is associated with poor treatment outcomes and reduced drug delivery. By analyzing collagen subtypes in PDAC tumors following different treatment regimens, immunofluorescence profiling can predict long-term survival and tumor growth inhibition. This information can aid in personalized treatment and improve patient survival.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Fatemeh Malekpour, Rashin Bahrami, Mahshid Hodjat, Neda Hakimiha, Behnam Bolhari, Aidin Sooratgar, Soheil Niavarzi
Summary: This study investigated the effect of photobiomodulation (PBM) therapy using an 808 nm diode laser on cellular modulation mechanisms in regenerative endodontic procedures (REPs). The results showed that PBM treatment significantly increased cell viability and migration, and enhanced the release of TGF-131 from dentin.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Pranathi Tata, Ramakrishnan Ganesan, Jayati Ray Dutta
Summary: Thin film coatings with potent antibacterial properties are widely used in medical devices, frequently touched surfaces, and food packaging. However, achieving antibacterial performance under both light and dark conditions remains a challenge. In this study, we successfully prepared AgBr-coated ZnO nanorod thin film architecture using a unique surfactant-mediated solution-phase spin-coating approach. The resulting structures showed dual functionality as antimicrobial agents and photosensitizers, effectively enhancing the visible-light photodisinfection efficacy of ZnO nanorods. The concentration of the surfactant-based precursor solution could be modulated to control the AgBr content. Additionally, components like urea and lactic acid were found to enhance the photocatalytic efficiency of the structures.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2024)