Article
Biophysics
Hyo Yong Kim, Jayeon Song, Hyun Gyu Park
Summary: An ultrasensitive isothermal method for detecting microRNA using target-induced chain amplification reaction (CAR) is described. The strategy involves a hairpin probe (HP) that opens upon binding to target miRNA, leading to exponential amplification reactions and the production of double-stranded DNA products that can be monitored through fluorescent signals.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Ian T. Hull, Enos C. Kline, Gaurav K. Gulati, Jack Henry Kotnik, Nuttada Panpradist, Kamal G. Shah, Qin Wang, Lisa Frenkel, James Lai, Joanne Stekler, Barry R. Lutz
Summary: The study presents a novel quantitative detection method for HIV DNA or RNA using a combination of recombinase polymerase amplification and lateral flow detection technologies, allowing for rapid and accurate testing in low-resource healthcare settings, with the potential to improve the quality of HIV treatment.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Yujin Jung, Jayeon Song, Hyun Gyu Park
Summary: The PHAmp method described in this study utilizes a phosphorothioated hairpin probe to assist in isothermal amplification, allowing for the detection of target nucleic acid down to 0.29 fM with a wide dynamic range and excellent specificity. The unique design principle may provide insights for the development of novel isothermal amplification methods for nucleic acid detection.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Gun-Soo Park, Jin-Soo Maeng
Summary: We developed a method for amplifying kilobases-long target sequence from RNA templates using Cre recombinase to generate circular intermediate template for subsequent amplification. The method can amplify a target sequence of about 2.9 kb, and the amplicon can be used for cloning.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Xiaonan Liu, Jiaxing Zhang, Yu Cai, Sinong Zhang, Kang Ma, Kai Hua, Yali Cui
Summary: This research focuses on developing a novel biosensor using LAMP-LFD technology to detect trace of DNA methylation from heterogeneous cancerous specimens, addressing the complexity in current DNA methylation detection. By introducing new labeling methods and gold magnetic nanoparticles, the biosensor is able to sensitively, rapidly, and accurately identify target variants.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Nanoscience & Nanotechnology
Min Hou, Xiang Yin, Jianhui Jiang, Jianjun He
Summary: The physically cross-linked DNAzyme-based hydrogel strategy enables specific envelopment and release of targeted cancer cells, allowing for enrichment and subsequent applications. This method offers an attractive approach for isolating viable cancer cells from various cell mixtures for downstream applications.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
B. Safeenaz Alladin-Mustan, Yuning Liu, Yimeng Li, Daria R. Q. de Almeida, Jesse Yuzik, Camilla F. Mendes, Julianne M. Gibbs
Summary: The study introduces a novel RT-LIDA method that utilizes T4 DNA ligase for RNA detection at room temperature. It can detect target RNA as low as 100 amol and distinguish it from total cellular RNA. The resulting DNA amplicons can be colorimetrically detected using DNA-modified gold nanoparticles at room temperature, allowing for instrument-free POC testing.
ANALYTICA CHIMICA ACTA
(2021)
Article
Food Science & Technology
Xu Chen, Yue Ma, Shuangyu Miao
Summary: A visual CAMP assay based on the ipaH gene was developed for rapid detection of Shigella in milk, and a simple paper-based DNA extraction method was established. The combination of these two methods showed higher sensitivity and specificity for on-site detection of Shigella in milk samples.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Chi Yao, Rui Zhang, Jianpu Tang, Dayong Yang
Summary: DNA hydrogels have unique properties and can be synthesized efficiently using rolling circle amplification.
Article
Multidisciplinary Sciences
Radhika Biyani, Kirti Sharma, Kenji Kojima, Madhu Biyani, Vishnu Sharma, Tarun Kumawat, Kevin Maafu Juma, Itaru Yanagihara, Shinsuke Fujiwara, Eiichi Kodama, Yuzuru Takamura, Masahiro Takagi, Kiyoshi Yasukawa, Manish Biyani
Summary: This new isothermal nucleic acid amplification assay, called RICCA, allows for rapid and direct detection of viruses with low viral loads. It has shown high sensitivity in detecting RNA viruses directly from saliva samples and synthetic templates of SARS-CoV-2. The assay can be used in real-time point-of-care testing and has a total assay time of 15-30 minutes for detecting heat-inactivated SARS-CoV-2 virus in human saliva.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Wakana Oda, Keisuke Umemura, Koichi Ito, Yusuke Kawamoto, Yuki Takahashi, Yoshinobu Takakura
Summary: This study developed a stable long single-stranded DNA (lssDNA) using i-motifs in an acidic environment as a carrier for CpG DNA, which showed potent immunostimulatory effects in murine macrophages and has the potential to be a novel adjuvant for immunization.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Analytical
Guohui Xue, Yinghao Cheng, Huo Xu, Chang Xue
Summary: DNA nanotechnology holds promise for biosensing and molecular recognition, but conventional DNA biosensors face limitations such as inadequate target stimuli and susceptibility to degradation. To overcome these constraints, a starlike branched and multiplex embedded system (SBES) with integrated functional design and cascade amplification is proposed for serum microRNA detection. The SBES exhibits enhanced sensitivity and low false-positive signals, and can be upgraded for multifunctional extension, opening up opportunities for versatile DNA nanostructures for biosensing.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Mengxu Sun, Yanxin Zhang, Juan Xie, Ya Zhang, Ting Huang, Minmin Li, Jin-Xiang Chen, Zong Dai, Jun Chen
Summary: This study developed a highly efficient and sensitive miRNA detection method by combining APE1-mediated isothermal target cycling amplification system with magnetic separation. The label-free and rapid detection of miRNA was achieved through the recognition and cleavage of AP probes by APE1, and the fluorescence measurement of G4/ThT. The method showed high specificity and sensitivity, and demonstrated its application in detecting miR-1246 in serum samples from breast cancer patients and normal people.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Sara Martorell, Luis Antonio Tortajada-Genaro, Miguel Angel Gonzalez-Martinez, Angel Maquieira
Summary: This study introduced a strategy for bioconjugation of probes to develop high-performance versatile chips for DNA biosensing. The use of dendrimer-oligonucleotide hybrids enabled sensitive detection of single-nucleotide variants, with a detection limit of 5 pM. The research demonstrated that high-density immobilization of these hybrids on thermoplastic surfaces outperformed other functionalization methods, leading to successful discrimination of a single-nucleotide mutant variant in colorectal tissues of cancer patients.
MICROCHEMICAL JOURNAL
(2021)
Article
Multidisciplinary Sciences
Gaolian Xu, Julien Reboud, Yunfei Guo, Hao Yang, Hongchen Gu, Chunhai Fan, Xiaohua Qian, Jonathan M. Cooper
Summary: Accelerating the design of nucleic acid amplification methods is challenging, but necessary for diagnosing diseases. This study presents a generalizable isothermal nucleic acid amplification methodology that can be applied to clinical settings, allowing for the detection of short miRNAs and difficult pathogenic targets.
NATURE COMMUNICATIONS
(2022)
Article
Nanoscience & Nanotechnology
Jingxian Wu, Honggeun Shin, Jeehee Lee, Soomi Kim, Haeshin Lee
Summary: In this study, a gelatin derivative gelatin-catechol (Gel-C) was developed to optimize its properties such as thermal reversibility and injectability. Gel-C showed temperature-dependent gelation capability and could form irreversible hydrogels without external stimuli. By controlling temperature and pH, the properties of Gel-C hydrogels could be manipulated, and an in situ gelling system for drug delivery was successfully fabricated by lowering the local temperature with cold shock, showing promising potential for targeted molecular delivery.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Chemistry, Medicinal
Suji Kwon, Minseon Kwon, Seongeun Im, Kyuri Lee, Hyukjin Lee
Summary: Synthetic mRNA has emerged as a promising biotherapeutic agent, especially in the development of mRNA vaccines. It allows fast, scalable, and cost-effective production, and has shown unprecedented impact in the fight against the COVID-19 pandemic. This review summarizes the latest technology and the innate immunogenicity of in vitro transcribed (IVT) mRNA, and discusses its role in vaccine development and future directions.
ARCHIVES OF PHARMACAL RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Swarup Kumar Maji, Subin Yu, Eunshil Choi, Ju Won Lim, Dohyub Jang, Ga-young Kim, Sehoon Kim, Hyukjin Lee, Dong Ha Kim
Summary: In this study, a multifunctional nanohybrid material based on gold nanorods was developed for cancer diagnosis and treatment. The material exhibited stability and protective properties, enabling real-time near-infrared imaging and synergistic therapeutic effects.
Article
Engineering, Biomedical
Jeehee Lee, Eunsook Park, Kyueui Lee, Mikyung Shin, Soohyeon Lee, Miguel Angel Moreno-Villaecija, Haeshin Lee
Summary: Plants release phenolic molecules for protection, and we designed an adhesive sealant inspired by the phenol-saccharide interactions in plant defense systems. By screening different saccharides, the O-acetyl group in glucomannan showed strong binding with tannic acid. This interaction exhibited pH and temperature sensitivities and could be used to prepare materials of various dimensions for printing techniques.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Hee Jin, Michaela Jeong, Gyeongseok Lee, Minjeong Kim, Youngjo Yoo, Hyun Jin Kim, Jaeho Cho, Yun-Sil Lee, Hyukjin Lee
Summary: In this study, engineered lipid nanoparticles (LNPs) were developed for delivering RNA therapeutics to the lungs, showing potential as a new treatment regimen for pulmonary fibrosis.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Jingxian Wu, Jaemeun Lee, Joo Young Jung, Jeong Ho Hwang, Ki-Suk Kim, Mikyung Shin, Haeshin Lee, Sun-Hyun Park
Summary: Blood vessel anastomosis by suture is a commonly used life-saving technique, but it is time-consuming and labor-intensive. This study proposes practical less-suture strategies using adhesive films for anastomosis, which reduces the number of stitches required and saves operating time. The decreased stitches also help alleviate fibrosis-mediated wall-thickening. This strategy is particularly useful for emergency situations and anastomosis of small-diameter vessels.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yu Ri Nam, Helen H. Ju, Jeehee Lee, Daiheon Lee, Yoojin Kim, Sung Jin Lee, Hong Kee Kim, Jae-Hyung Jang, Haeshin Lee
Summary: Recently, AAV therapy for incurable diseases has been introduced due to its safety properties and neutralizing antibodies. However, the mixture of empty and full capsids in AAV production increases viral doses to humans. TEM, the primary tool for distinguishing between empty and full capsids, creates research limitations. This study demonstrates that AFM can be an alternative tool to TEM, allowing the distinct height and morphological differences between empty and full AAV particles.
Article
Biotechnology & Applied Microbiology
Minjeong Kim, Michaela Jeong, Gyeongseok Lee, Yeji Lee, Jeongeun Park, Hyein Jung, Seongeun Im, Joo-Sung Yang, Kyungjin Kim, Hyukjin Lee
Summary: mRNA-based protein replacement therapy is a promising intervention for clinical disease treatment, but the immunogenicity of lipid nanoparticles (LNPs) has limited their translation to the clinic. In this study, a novel ionizable LNP with enhanced potency and safety was developed.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Engineering, Biomedical
Maria P. P. Sousa, Catarina T. T. Passos, Marian Fuersatz, Haeshin Lee, Sonia G. Patricio, Joao F. Mano, Sylvia Nuernberger
Summary: Articular cartilage injuries are a major health concern due to their limited self-renewal capacity, leading to increased economic burden in the healthcare system. The development of cell implantation strategies using a suitable delivery platform holds promise for increasing cell retention and providing sufficient cells for cartilage repair. This study reports the development of anisotropically patterned mussel-inspired membranes with nanotopography and catechol molecular cues for efficient cell delivery. The membranes demonstrate successful cell migration and retention on cartilage surfaces, making them a promising strategy for cell-based therapies.
ADVANCED NANOBIOMED RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Michaela Jeong, Yeji Lee, Jeongeun Park, Hyein Jung, Hyukjin Lee
Summary: RNA therapeutics have made significant breakthroughs in treating incurable diseases and genetic disorders by regulating gene expression. The development of COVID19 mRNA vaccines highlights the potential of RNA therapeutics in preventing infectious diseases and treating chronic diseases. However, efficient delivery of RNA into cells remains a challenge, and lipid nanoparticles (LNPs) are necessary for successful RNA therapeutics. This review focuses on the fundamental aspects of LNPs, their uses, recent advances, and breakthrough technologies to overcome limitations.
ADVANCED DRUG DELIVERY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Jisoo Shin, Soohwan An, Soojeong Choi, Mikyung Shin, Jung Seung Lee, Jung Ho Cho, Haeshin Lee, Seung-Woo Cho
Summary: This study highlights the important role of ferritin in the self-healing lifetime of soft phenolic materials. Ferritin interacts with a catechol-functionalized polymer to form a self-healable and adhesive hydrogel bidirectionally by providing and retrieving Fe3+. The unique role of ferritin in storing and releasing iron significantly increases the self-healing lifetime of the hydrogel compared to direct Fe3+ addition without ferritin. Ferritin also induces stable oxidative coupling between catechol moieties, contributing to double cross-linking networks and broadening the versatility of phenolic hydrogels in biomedical applications.
Article
Multidisciplinary Sciences
Jun Sung Kim, Sang-Kyu Park, Haeshin Lee
Summary: Additive manufacturing, or 3D printing, has revolutionized object creation, but it may lead to increased local defects compared to traditional casting methods. Factors like light intensity and temperature fluctuations contribute to defect formations. Detecting and locating toxic sources in 3D printed materials is a challenge, and current toxicological assessments rely on passive detection. Introducing the nematode model organism, C. elegans, offers an active system for identification and localization due to its unique sensing and locomotion capabilities.
Article
Medicine, Research & Experimental
Jeong Pil Han, Yeji Lee, Jeong Hyeon Lee, Hye Yoon Chung, Geon Seong Lee, Yu Ri Nam, Myeongjin Choi, Kyoung-Sik Moon, Haeshin Lee, Hyukjin Lee, Su Cheong Yeom
Summary: Gene editing has been successfully used to develop an effective, safe, and sustainable treatment for hemophilia A. By targeting specific gene sites and using a combined rebalancing strategy, this method improves blood coagulation activity, reduces complications, and enhances survival in mice with hemophilia.
MOLECULAR THERAPY NUCLEIC ACIDS
(2023)
Review
Biochemistry & Molecular Biology
Yeji Lee, Michaela Jeong, Jeongeun Park, Hyein Jung, Hyukjin Lee
Summary: This article provides an overview of the immunological response to lipid nanoparticles and discusses the adverse effects and strategies for regulating the immunogenicity associated with these nanoparticles. Lipid nanoparticles have been utilized to enhance mRNA therapeutics and vaccines, but their recognition as foreign materials by the body leads to innate immune response, impacting adaptive immunity. Understanding the specific type of innate immune response triggered by lipid nanoparticles is crucial.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Jongmin Park, Eunsook Park, Siyoung Q. Choi, Jingxian Wu, Jihye Park, Hyeonju Lee, Hyungjun Kim, Haeshin Lee, Myungeun Seo
Summary: Bioadhesives are becoming increasingly important in medical science. However, developing adhesive materials that combine strong adhesion, biocompatibility, and biodegradation remains challenging. In this study, a biocompatible and biodegradable block copolymer-based waterborne superglue was developed for follicle-free hair transplantation. The adhesive showed step-wise increase in adhesion strength and significantly enhanced mechanical properties compared to the conventional formulation.
