Differences in foliar phosphorus fractions, rather than in cell-specific phosphorus allocation, underlie contrasting photosynthetic phosphorus use efficiency among chickpea genotypes

Optimized allocation to specific foliar phosphorus fractions, rather than preferential allocation of phosphorus to specific leaf tissues (i.e. mesophyll versus epidermis), underlies differences in photosynthetic phosphorus use efficiency among chickpea genotypes. Although significant intraspecific variation in photosynthetic phosphorus (P) use efficiency (PPUE) has been shown in numerous species, we still know little about the biochemical basis for differences in PPUE among genotypes within a species. Here, we grew two high PPUE and two low PPUE chickpea (Cicer arietinum) genotypes with low P supply in a glasshouse to compare their photosynthesis-related traits, total foliar P concentration ([P]) and chemical P fractions (i.e. inorganic P (Pi), metabolite P, lipid P, nucleic acid P, and residual P). Foliar cell-specific nutrient concentrations including P were characterized using elemental X-ray microanalysis. Genotypes with high PPUE showed lower total foliar [P] without slower photosynthetic rates. No consistent differences in cellular [P] between the epidermis and mesophyll cells occurred across the four genotypes. In contrast, high PPUE was associated with lower allocation to Pi and metabolite P, with PPUE being negatively correlated with the percentage of these two fractions. Furthermore, a lower allocation to Pi and metabolite P was correlated with a greater allocation to nucleic acid P, but not to lipid P. Collectively, our results suggest that a different allocation to foliar P fractions, rather than preferential P allocation to specific leaf tissues, underlies the contrasting PPUE among chickpea genotypes.

Automated summary

The difference in photosynthetic phosphorus use efficiency (PPUE) among chickpea genotypes is attributed to optimized allocation to specific foliar phosphorus fractions rather than preferential allocation to specific leaf tissues. Genotypes with high PPUE show lower total foliar phosphorus concentration without slower photosynthetic rates. This study highlights the importance of allocation to different phosphorus fractions in determining PPUE.