Auditory or Audiovisual Stimulation Ameliorates Cognitive Impairment and Neuropathology in ApoE4 Knock-In Mice

We hypothesized that auditory stimulation could reduce the progression of Alzheimer's disease (AD), and that audiovisual stimulation could have additional effects through multisensory integration. We exposed 12 month old Apoe(tm1.1(APOE*4)Adiuj) mice (a mouse model of sporadic AD) to auditory (A) or audiovisual stimulation (AV) at 40 Hz for 14 days in a soundproof chamber system (no stimulation, N). Behavioral tests were performed before and after each session, and their brain tissues were assessed for amyloid-beta expression and apoptotic cell death, after 14 days. Furthermore, brain levels of acetylcholine and apoptosis-related proteins were analyzed. In the Y-maze test, the percentage relative alternation was significantly higher in group A than in group N mice. Amyloid-beta and TUNEL positivity in the hippocampal CA3 region was significantly lower in group A and group AV mice than in group N mice (p < 0.05). Acetylcholine levels were significantly higher in group A and group AV mice than in group N mice (p < 0.05). Compared to group N mice, expression of the proapoptotic proteins Bax and caspase-3 was lower in group A, and expression of the antiapoptotic protein Bcl-2 was higher in group AV. In a mouse model of early-stage sporadic AD, auditory or audiovisual stimulation improved cognitive performance and neuropathology.

Automated summary

This study conducted an experiment with a mouse model of Alzheimer's disease, and found that auditory stimulation could slow down the progression of the disease, while audiovisual stimulation had additional effects through multisensory integration. The mice in the auditory and audiovisual stimulation groups showed better performance in behavioral tests and lower levels of amyloid-beta and apoptotic cell death in their brain tissues. These findings provide new insights for the treatment of early-stage Alzheimer's disease.