Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/ijms24032849
Keywords
tyrosine kinase inhibitor; COVID-19; proton dissociation; lipophilicity; plasma protein binding; binding constants
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Uncertainties exist in plasma protein binding and blood distribution of the COVID-19 therapeutics favipiravir (FAVI), molnupiravir (MOLNU), and imatinib (IMA). This study investigated the proton dissociation processes, solubility, lipophilicity, and serum protein binding of these drugs. The results showed different degrees of lipophilicity at different pH values. MOLNU was hydrolyzed by blood serum proteins, while FAVI had weak binding to blood proteins and IMA showed high affinity binding to AGP.
There are a number of uncertainties regarding plasma protein binding and blood distribution of the active drugs favipiravir (FAVI), molnupiravir (MOLNU) and imatinib (IMA), which were recently proposed as therapeutics for the treatment of COVID-19 disease. Therefore, proton dissociation processes, solubility, lipophilicity, and serum protein binding of these three substances were investigated in detail. The drugs display various degrees of lipophilicity at gastric (pH 2.0) and blood pH (pH 7.4). The determined pK(a) values explain well the changes in lipophilic character of the respective compounds. The serum protein binding was studied by membrane ultrafiltration, frontal analysis capillary electrophoresis, steady-state fluorometry, and fluorescence anisotropy techniques. The studies revealed that the ester bond in MOLNU is hydrolyzed by protein constituents of blood serum. Molnupiravir and its hydrolyzed form do not bind considerably to blood proteins. Likewise, FAVI does not bind to human serum albumin (HSA) and alpha 1-acid glycoprotein (AGP) and shows relatively weak binding to the protein fraction of whole blood serum. Imatinib binds to AGP with high affinity (logK ' = 5.8-6.0), while its binding to HSA is much weaker (logK ' <= 4.0). The computed constants were used to model the distribution of IMA in blood plasma under physiological and 'acute-phase' conditions as well.
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