Journal
EXPERIMENTAL EYE RESEARCH
Volume 225, Issue -, Pages -Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exer.2022.109271
Keywords
Glaucoma; Glutamate excitatory toxicity; Mitochondrial dynamics; Retinal neurons; DRP1
Categories
Funding
- National Natural Science Foundation of China
- Key R & D plan of Hunan Province of China
- National key research and develop-ment program of China
- [81670858]
- [81974134]
- [2020SK2076]
- [2020YFC2008205]
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This study found that in the glutamate excitotoxicity model, retinal neurons can promote mitochondrial fusion by reducing the phosphorylation of ERK1/2 and its downstream protein DRP1 S585, and enhance their ability to resist the excitotoxicity of glutamate.
There are many theories surrounding the pathogenesis of glaucoma, and glutamate excitatory toxicity has been suggested to play an important role. Some studies have shown that glutamate excitatory toxicity is associated with mitochondrial dynamics; however, the relationship between glutamate excitatory toxicity and mitochon-drial dynamics in the pathogenesis of glaucoma remains unclear. In this study, the glutamate transporter in-hibitor, threohydroxyaspartate, was used to simulate the glutamate excitatory toxicity cell model of rat retinal neurons in vitro, and the changes in the level of proteins related to mitochondrial dynamics, mitochondrial morphology, and length of neuronal axons were measured. We found that in the glutamate excitotoxicity model, retinal neurons can promote mitochondrial fusion by reducing the phosphorylation of ERK1/2 and its down-stream protein DRP1 S585, and enhance its ability to resist the excitotoxicity of glutamate. At the same time, the DRP1-specific inhibitor, Mdivi-1, could promote the mitochondrial fusion of retinal neurons.
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