Article
Multidisciplinary Sciences
Lilian Hor, Akila Pilapitiya, James A. McKenna, Santosh Panjikar, Marilyn A. Anderson, Mickael Desvaux, Jason J. Paxman, Begona Heras
Summary: Researchers reveal the structural features of the bacterial protein autotransporter Ssp and its association with cytotoxic function.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Mingbo Qu, Xiaoxi Guo, Shuang Tian, Qing Yang, Myeongjin Kim, Seulgi Mun, Mi Young Noh, Karl J. Kramer, Subbaratnam Muthukrishnan, Yasuyuki Arakane
Summary: This study reveals the catalytic activity and physiological importance of LPMO15-1-like proteins from insects, providing new insight into the enzymatic mechanism of cuticular chitin turnover during molting.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
H. Sasimali M. Soysa, Sawitree Kumsaoad, Rawiporn Amornloetwattana, Takeshi Watanabe, Wipa Suginta
Summary: Serratia marcescens is an opportunistic pathogen that can utilize chitin as a carbon source. It produces chitin-degrading enzymes and membrane transporters to digest and transport chitin degradation products. In this study, the properties and function of the chitooligosaccharide transporter, SmChiP, were investigated. SmChiP is a monomeric porin that forms a stable channel and exhibits substrate specificity towards chitooligosaccharides. It may serve as a target for the development of antimicrobial therapies against multidrug antibiotic-resistant S. marcescens infections.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Microbiology
Xianbo Jia, Ke Zhao, Fangchen Liu, Junjie Lin, Chenqiang Lin, Jichen Chen
Summary: This study reveals the regulatory mechanism of prodigiosin production by the transcription factor OmpR. OmpR positively regulates prodigiosin production by directly binding to the promoter region of the prodigiosin biosynthesis cluster. Additionally, OmpR also regulates its own expression, further affecting prodigiosin production.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ashmeet Singh, Jojo P. Joseph, Deepika Gupta, Chirag Miglani, Nimisha A. Mavlankar, Asish Pal
Summary: Peptides, as alternative catalysts to enzymes, self-assemble into nanostructures through external cues, mimicking the activity of hydrolase enzymes. The peptide nanostructures exhibit robust hydrolase activity over a broad range of temperature and pH.
Article
Forestry
Ping Zhang, Qingquan Zhao, Xiaoqian Ma, Ling Ma
Summary: The study revealed that Serratia marcescens Bizio significantly shortened the lifespan of hazelnut weevil, impacting larval weight and digestive enzyme activity. This bacterium destroyed midgut cells, interfered with digestive and protective enzymes, leading to reduced food intake and increased mortality of hazelnut weevil.
JOURNAL OF FORESTRY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Tania Paul, Abhijit Mondal, Tarun Kanti Bandyopadhyay, Nibedita Mahata, Biswanath Bhunia
Summary: The present study aims to optimize various process parameters during ultrasound-assisted extraction (UAE) to increase the yield of prodigiosin. The Taguchi methodology is used to determine the optimal conditions, which resulted in a significant increase in prodigiosin yield. Additionally, a second-order kinetic model is used to create a batch extractor for commercial extraction of prodigiosin.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Faizan Abul Qais, Mohammad Shavez Khan, Iqbal Ahmad, Fohad Mabood Husain, Rais Ahmad Khan, Iftekhar Hassan, Syed Ali Shahzad, Walaa AlHarbi
Summary: This study demonstrated the significant inhibitory effect of coumarin on QS-regulated virulent traits of Gram-negative bacteria, including the suppression of various virulence factors and biofilm formation. Computational studies revealed that coumarin may inhibit quorum sensing activity through multiple mechanisms.
Article
Energy & Fuels
Tania Paul, Abhijit Mondal, Tarun Kanti Bandyopadhyay, Biswanath Bhunia
Summary: Prodigiosin, a red pigment with various properties, was studied in terms of media types, temperature, and nitrogen and carbon sources to improve yield. Specific conditions led to increased production of Prodigiosin in Serratia marcescens, with lactose and peptone identified as key sources.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biochemistry & Molecular Biology
C. Chinmayee, Asha Martin, B. S. Gnanesh Kumar, Sridevi Annapurna Singh
Summary: This study describes the production of a new thermostable fungal aspartic protease through solid-state fermentation from Rhizopus azygosporus, as well as its purification and characterization. The enzyme exhibited unusually high thermostability and specific enzyme activity, making it a potential candidate for various industrial applications.
PROCESS BIOCHEMISTRY
(2022)
Article
Chemistry, Medicinal
Henry R. O'Donnell, Tia A. Tummino, Conner Bardine, Charles S. Craik, Brian K. Shoichet
Summary: This study reveals that many drugs repurposed for combating COVID-19 in biochemical assays are actually artifacts, and suggests that even drugs can act artifactually via colloidal aggregation at screening-relevant concentrations.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Yulong Wei, Ning Yu, Yue Zhu, Jingyi Hao, Junyan Shi, Yuqing Lei, Zhilin Gan, Guoliang Jia, Chao Ma, Aidong Sun
Summary: The first purification of three PPOs from blueberries was achieved, with their optimal conditions and activity changes under different treatments being discovered. Additionally, the binding of substrate to catalytic cavity was found to be related to certain amino acid residues.
Article
Chemistry, Applied
Dandan Li, Caiwen Chen, Yang Tao, Yi Huang, Pei Wang, Yongbin Han
Summary: The study found that moderate electric field can affect the performance of alpha-amylase, increasing enzyme activity and promoting starch hydrolysis. The substrate structure plays a dominant role in the enzymatic hydrolysis of starch under MEF, providing guidance for the application of electro-based techniques in starch modification.
FOOD HYDROCOLLOIDS
(2022)
Article
Multidisciplinary Sciences
Chongyang Wang, Changshui Liu, Xiaochuan Zhu, Quancai Peng, Qingjun Ma
Summary: This study reveals the structural flexibility of Vibrio dual lipases/transferases and proposes a mechanism for tuning the catalytic site to explain enzyme promiscuity. Enzyme promiscuity, the ability to catalyze multiple substrates and reactions, is widely observed but poorly understood. The researchers discovered a flexible catalytic triad machinery in Vibrio dual lipase/transferase that can undergo conformational changes to facilitate substrate and catalytic promiscuity.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Asma Zafar, Ziaur Rahman, Hira Mubeen, Javairia Makhdoom, Javeria Tariq, Mahjabeen, Zulqurnain Ali, Attia Hamid, Eeza Shafique, Muhammad Nauman Aftab
Summary: This study aimed to clone and express the esterase gene from Bacillus thuringiensis and investigate its purification, stability, and activity. The results showed that the recombinant esterase enzyme had high activity, stability, and resistance, which are significant for the detergent industry.
Article
Genetics & Heredity
Bo Xu, Yingzhe Wang, Haizhu Zhou, Changlong Gou, Wenlong Dong, Yu Wang, Yunhang Gao, Hongxia Ma
MITOCHONDRIAL DNA PART B-RESOURCES
(2016)
Article
Veterinary Sciences
Wen-Long Dong, Ling-Cong Kong, Yu Wang, Chang-Long Gou, Bo Xu, Hong-Xia Ma, Yun-Hang Gao
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2017)
Article
Fisheries
Changlong Gou, Jiazhen Wang, Yuqiong Wang, Wenlong Dong, Xiaofeng Shan, Yujie Lou, Yunhang Gao
FISH & SHELLFISH IMMUNOLOGY
(2018)
Article
Entomology
Bo Xu, Yingzhe Wang, Shixin Zhu, Haizhu Zhou, Changlong Gou, Wenlong Dong, Yu Wang, Yunhang Gao, Hongxia Ma
ENTOMOLOGICAL RESEARCH
(2019)
Article
Agricultural Engineering
Changlong Gou, Yuqiong Wang, Xiqing Zhang, Rongzhen Zhong, Yunhang Gao
Summary: The addition of Chlorotetracycline (CTC) to cattle manure composting systems hindered the removal of antibiotic-resistant genes (ARGs) and increased the relative abundance (RA) of certain ARGs and mobile genetic elements (MGEs). High levels of CTC had a more persistent effect on bacterial community and Firmicutes and Proteobacteria were identified as the main hosts for ARGs based on network analysis. The presence of CTC increased the risks of spreading ARGs in compost products.
BIORESOURCE TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Yuqiong Wang, Yang Luo, Lilong Luo, Hang Zhang, Yangci Liao, Changlong Gou
Summary: Among the four Pleurotus spp. fungi studied, P. sajor-caju and P. eryngii showed better potential in improving the nutritional value of corn stover as ruminant feed. P. citrinopileatus treatment resulted in the highest crude protein content, while P. sajor-caju treatment led to the highest increase in essential amino acids. Overall, P. eryngii and P. sajor-caju enhanced the nutritive value of corn stover as ruminant feed.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Peng Hao, Sicheng Wu, Xiqing Zhang, Changlong Gou, Yuqiong Wang, Lixia Wang, Yanbin Zhu, Wangdui Basang, Yunhang Gao
Summary: A highly efficient E2-degrading bacterial strain, Microbacterium resistens MZT7, was isolated and characterized. The strain showed excellent adaptability and degradation efficiency towards E2. Three metabolic pathways were proposed and one pathway was confirmed through gene identification. This study provides important insights into the degradation of E2.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Microbiology
Sicheng Wu, Peng Hao, Changlong Gou, Xiqing Zhang, Lixia Wang, Wangdui Basang, Yanbin Zhu, Yunhang Gao
Summary: This study identified a strain of Lysinibacillus sp. named GG242 that is capable of efficiently degrading environmental estrogen. The degradation ability of strain GG242 is not influenced by temperature, pH, or salinity. Analysis of transformation products suggests that E2 can be converted via two distinct routes.
Article
Environmental Sciences
Peng Hao, Zongshuo Lv, Sicheng Wu, Xiqing Zhang, Changlong Gou, Lixia Wang, Yanbin Zhu, Wangdui Basang, Yunhang Gao
Summary: 17-beta-estradiol (E2) pollution is a major concern due to its risks to the environment and human health. However, the mechanism of microbial degradation of E2 is not well understood. This study investigated the location of E2-degrading enzymes and conducted transcriptome analysis of Microbacterium resistens MZT7 (M. resistens MZT7) exposed to E2. The results revealed differentially expressed genes (DEGs) related to transport, metabolism, and stress response, providing new insights into the molecular mechanism of E2 biotransformation by M. resistens MZT7.
ENVIRONMENTAL RESEARCH
(2023)
Article
Environmental Sciences
Xintian Li, Wei Zhai, Xinran Duan, Changlong Gou, Min Li, Lixia Wang, Wangdui Basang, Yanbin Zhu, Yunhang Gao
Summary: This study isolated a Cellulosimicrobium sp. strain, named L1, which can transform sulfide. The extracellular enzyme plays a major role in this process. The purified sulfur convertase has thermal and storage stability, and is not significantly affected by inhibitors and organic solvents. The sulfur convertase can completely remove sulfide in livestock wastewater.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Agricultural Engineering
Changlong Gou, Yuqiong Wang, Xiqing Zhang, Yujie Lou, Yunhang Gao
BIORESOURCE TECHNOLOGY
(2017)