Article
Biochemistry & Molecular Biology
Qiuling Liu, Tujing Song, Bing Chen, Jingjing Zhang, Wen Li
Summary: Hypoxia is crucial for the development of various disorders, especially hypoxic cerebropathy. The damage to the blood-brain barrier (BBB) caused by hypoxia is well known. This study explored the correlation between endothelial ferroptosis and hypoxia-induced BBB damage using in vivo zebrafish and in vitro bEnd.3 cells. The results showed that hypoxic treatment could induce BBB disruption by down-regulating claudin-5 (CLDN5) expression in both zebrafish cerebrovascular endothelial cells and bEnd.3 cells.
Article
Food Science & Technology
Jin Li, Yaqiong Jian, Ruonan Liu, Xianfeng Zhao, Jiangyi Mao, Wei Wei, Chenyu Jiang, Lina Zhang, Yi Wang, Peng Zhou
Summary: This study found that combined intake of fish oil and choline can increase the uptake of DHA in the brain, as well as enhance the synthesis and release of acetylcholine, thereby improving learning and memory abilities in mice.
Article
Biochemistry & Molecular Biology
Jiang Fang, Qiang Yuan, Zhuoying Du, Maoxing Fei, Quan Zhang, Lei Yang, Meihua Wang, Weijian Yang, Jian Yu, Gang Wu, Jin Hu
Summary: Ferroptosis plays an important role in traumatic brain injury (TBI) and is involved in the damage of brain microvascular endothelial cells and the blood-brain barrier. In this study, they found that ferroptosis-related markers were altered in brain endothelial cells after TBI both in vitro and in vivo. Additionally, inhibition of ferroptosis with Fer-1 reduced BMVECs death, BBB permeability, and tight junction loss, and exerted neuroprotective effects in the early stage of TBI.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Immunology
Rui-Cheng Yang, Kun Huang, Hui-Peng Zhang, Liang Li, Yu-Fei Zhang, Chen Tan, Huan-Chun Chen, Mei-Lin Jin, Xiang-Ru Wang
Summary: The study found that SARS-CoV-2 infection can cause dysfunction of the blood-brain barrier, increasing its permeability, which provides new insights into the neuropathogenesis of SARS-CoV-2.
JOURNAL OF NEUROINFLAMMATION
(2022)
Review
Nutrition & Dietetics
Amanda Lo Van, Nathalie Bernoud-Hubac, Michel Lagarde
Summary: DHA-LysoPC and AceDoPC are proposed as the main transporters of DHA to the brain. Esterification of DHA within these compounds can enhance their neuroprotective effects, with AceDoPC also possessing antioxidant properties and the ability to generate acetylcholine.
Article
Microbiology
Ronghua Zou, Xiaoni Wu, Qiwei Chen, Xiaowei Gong, Yuefeng Chu, Fuying Zheng
Summary: In this study, it was found that the OmpA protein of R. anatipestifer is an important virulence factor mediating its invasion of duck brain microvascular endothelial cells and penetration of the blood-brain barrier. The amino acids 230-242 of OmpA represent a key domain involved in R. anatipestifer invasion.
VETERINARY MICROBIOLOGY
(2023)
Article
Geriatrics & Gerontology
Alpna Tyagi, Carol Mirita, Iman Shah, P. Hemachandra Reddy, Subbiah Pugazhenthi
Summary: This study suggests that Sirt3 deficiency exacerbates the harmful effects of lipotoxicity on BMECs, leading to aggravated neuroinflammation.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Reena Jatyan, Deepak Kumar Sahel, Prabhjeet Singh, Rajeev Sakhuja, Anupama Mittal, Deepak Chitkara
Summary: In this study, the conjugation of fatty acids with temozolomide improved its anticancer potential against glioblastoma multiforme. The synthesized conjugates showed a stronger inhibitory effect on tumor cells in vitro and improved survival rate in an animal model.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Multidisciplinary Sciences
Tyler M. Lu, Sean Houghton, Tarig Magdeldin, Jose Gabriel Barcia Duran, Andrew P. Minotti, Amanda Snead, Andrew Sproul, Duc-Huy T. Nguyen, Jenny Xiang, Howard A. Fine, Zev Rosenwaks, Lorenz Studer, Shahin Rafii, Dritan Agalliu, David Redmond, Raphael Lis
Summary: Researchers have found that iBMECs lack functional attributes of ECs and instead express genes related to the neuroectodermal epithelial lineage. By overexpressing key endothelial ETS transcription factors, Epi-iBMECs can be reprogrammed into authentic endothelial cells that are congruent with bona fide endothelium at both transcriptomic and some functional levels.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Manjusha Thakar, Midrelle E. Noumbissi, Monique F. Stins
Summary: Postmortem neuropathological studies have revealed regional differences in the brain diseases, such as cerebral malaria, which shows more hemorrhagic punctae in the white matter than grey matter. The underlying cause of these differences is unclear. In this study, researchers investigated the impact of the vascular microenvironment on the expression of endothelial protein C receptor (EPCR) and found that EPCR expression in cerebral microvessels is heterogeneous in the white matter compared to the grey matter. Their findings provide insights into the origin of molecular phenotypic heterogeneity at the microvascular level and may contribute to a better understanding of variations in neuropathology associated with vasculature in different brain regions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Sorabh Sharma, Craig E. Brown
Summary: This review discusses the relationship between microvascular dysfunction caused by type 1 diabetes and cognitive impairment, aiming to synthesize the current knowledge in this field.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Acoustics
Ming-Yen Hsiao, Defei Liao, Gaoming Xiang, Pei Zhong
Summary: In this study, a unique VD-SAW system was used to apply vertically deployed surface acoustic waves (VD-SAWs) on human cerebral microvascular endothelial cells to investigate the interaction mechanisms between ultrasound and the blood-brain barrier (BBB). The results showed that VD-SAWs induced intracellular calcium surge and intercellular calcium waves, and the permeability of the BBB decreased with increasing pulse repetition frequency, possibly involving mechanosensitive ion channels. This study provides a useful platform for further mechanistic studies on ultrasound-mediated BBB opening.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Hinako Nagano, Shingo Ito, Takeshi Masuda, Sumio Ohtsuki
Summary: The study suggests that INSR-mediated signaling is involved in regulating the expression of functional proteins at the blood-brain barrier (BBB), contributing to BBB function maintenance. Changes in protein expressions, particularly those related to amyloid-beta peptides, in INSR knockdown cells may contribute to the development of cerebral amyloid angiopathy through the suppression of INSR-mediated signaling.
PHARMACEUTICAL RESEARCH
(2022)
Article
Chemistry, Medicinal
Hinako Nagano, Seiryo Ogata, Shingo Ito, Takeshi Masuda, Sumio Ohtsuki
Summary: This study reveals the role of Podocalyxin (PODXL) in blood-brain barrier function. PODXL knockdown affects immune response and intracellular trafficking, and regulates the protein expression and efflux activity of ABCB1/MDR1.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Neurosciences
Guneet Kaur, Pallavi Pant, Reshma Bhagat, Pankaj Seth
Summary: In this study, a physiologically relevant blood-brain barrier (BBB) model was established using human brain microvascular endothelial cells (hBMECs) and astrocytes derived from human progenitors. The effects of Zika virus envelope (E) protein on the cells comprising the BBB were investigated using this model. The presence of ZIKV E protein led to decreased expression of crucial endothelial junction proteins, increased BBB permeability, and inflammatory response in astrocytes.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Review
Chemistry, Analytical
Yada Nolvachai, Michelle S. S. Amaral, Philip J. Marriott
ANALYTICAL CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Sanjeevini Babu Reddiar, Michael de Veer, Brett M. Paterson, Tara Sepehrizadeh, Dorothy C. C. Wai, Agota Csoti, Gyorgy Panyi, Joseph A. Nicolazzo, Raymond S. Norton
Summary: This study investigates the in vivo distribution of HsTX1[R14A] peptide in mice and finds increased uptake in a LPS-induced mouse model of neuroinflammation, as well as accumulation in inflamed joints.
MOLECULAR PHARMACEUTICS
(2023)
Review
Biochemistry & Molecular Biology
Jessica-Ai-Lyn Yon, Sue-Kei Lee, Jing-Wen Keng, Sek-Chuen Chow, Kai-Bin Liew, Swee-Sen Teo, Wan Mastura Shaik Mossadeq, Philip J. Marriott, Gabriel Akyirem Akowuah, Long Chiau Ming, Bey Hing Goh, Yik-Ling Chew
Summary: This article is a review of atopic dermatitis (AD) management, focusing on the pharmacological effects and phytochemical content of Cassia alata. The antibacterial, wound healing, anti-inflammatory, and antioxidant effects of C. alata are discussed in relation to AD management.
Article
Biochemical Research Methods
Michelle S. S. Amaral, Milton T. W. Hearn, Philip J. Marriott
Summary: Candida antarctica lipase A (CALA) was used for chemo-selective enzymatic transesterification of terpene and phenyl alcohols in 35 different essential oil samples. The results showed high conversion rates for primary and secondary alcohols, while tertiary alcohols and phenols showed no significant conversion. The process demonstrated selectivity, efficiency, scalability, and potential reusability, making it a greener and more sustainable alternative for the production of novel flavors and fragrances in the industry.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Nuttanee Tungkijanansin, Yada Nolvachai, Puttaruksa Varanusupakul, Napida Hinchiranan, Chadin Kulsing, Philip J. Marriott
Summary: A non-cryogenic artificial trapping modulation technique, called M-SNAT, was developed using multiloop splitters and various columns. This technique achieved artificial splitting and trapping of analytes by utilizing split pulses, pulse delay, and their combination within each loop. The SNAT approach was successfully applied for modulating a wide range of compounds in waste tire pyrolysis samples, resulting in high precision and improved separation capacity.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Jiaqiang Luo, Jamie Selby-Pham, Kimber Wise, Yinhao Wu, Jiacan Sun, Yameng Qu, Tian Cao, Pangzhen Zhang, Philip J. Marriott, Kate Howell
Summary: Wine producers use berry morphology, taste, and basic chemical parameters to predict wine quality. Incorporating analysis on grape and wine volatiles can achieve more accurate predictions, but careful selection of grapes, controlled fermentations, and standardized assessments are required. Three models were presented for Shiraz wine quality prediction.
AUSTRALIAN JOURNAL OF GRAPE AND WINE RESEARCH
(2023)
Article
Medicine, Research & Experimental
Alina D. Lam, Ian K. Styles, Danielle Senyschyn, Enyuan Cao, Abel Anshabo, Mohammad Abdallah, Reyaj Mikrani, Cameron J. Nowell, Christopher J. H. Porter, Orlagh M. Feeney, Natalie L. Trevaskis
Summary: Changes in immune cells within the joint-draining lymphatics contribute to the progression of inflammatory arthritis. There is an expansion of pathogenic B cells in the joint-draining lymph node, which inhibit lymph flow and reduce clearance of inflammatory fluid and cells. Localized delivery of B-cell depleting therapies to the joint-draining lymph nodes has the potential to improve treatment efficacy and reduce side effects.
MOLECULAR PHARMACEUTICS
(2023)
Editorial Material
Medicine, Research & Experimental
Orlagh M. Feeney, Christopher J. H. Porter, Kara L. Vine
MOLECULAR PHARMACEUTICS
(2023)
Article
Clinical Neurology
Yijun Pan, Yoshiteru Kagawa, Jiaqi Sun, Deanna S. Deveson Lucas, Ryusuke Takechi, John C. L. Mamo, Dorothy C. C. Wai, Raymond S. Norton, Liang Jin, Joseph A. Nicolazzo
Summary: Increased expression of Kv1.3 in activated microglia is associated with neuroinflammation in Alzheimer's disease (AD), and inhibiting Kv1.3 has shown potential in improving cognitive function in AD mouse models. In this study, microglial expression of Kv1.3 was found to be increased in an AD animal model, and treatment with the Kv1.3 blocker HsTX1[R14A] significantly improved cognitive deficits. Transcriptomics analysis revealed changes in gene expression related to inflammation, neuron differentiation, synapse function, learning, and memory. These results highlight the potential of Kv1.3 blockade as a therapeutic strategy for AD.
Article
Biochemical Research Methods
Neil Andrew Byrnes, Yuesong Wu, Yada Nolvachai, Philip J. Marriott
Summary: This study compares the visual presentation of chromatographic data from the isomerisation of natural pyrethrins using one-dimensional gas chromatography (1DGC) and comprehensive two-dimensional gas chromatography (GCxGC). The results show that GCxGC provides a better recognition and analysis of the isomerisation process, aiding in analyte identification.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Review
Biochemical Research Methods
Humberto R. Bizzo, Nathalia S. Brilhante, Yada Nolvachai, Philip J. Marriott
Summary: The concept of retention indices (RI) is valuable in gas chromatography (GC) practice, providing a preliminary indication of possible molecules for compound identification. However, limitations and potential misuse should be recognized when calculating and using RI values.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Pharmacology & Pharmacy
Jae Pyun, Huijing Koay, Pranav Runwal, Celeste Mawal, Ashley I. Bush, Yijun Pan, Paul S. Donnelly, Jennifer L. Short, Joseph A. Nicolazzo
Summary: Cu(ATSM) enhances the expression and function of P-gp at the blood-brain barrier, which has important implications for CNS drug delivery and clearance of A beta in AD.
Article
Pharmacology & Pharmacy
Alexander J. Martin, David M. Shackleford, Susan A. Charman, Kylie M. Wagstaff, Christopher J. H. Porter, David A. Jans
Summary: This study evaluated the in vivo exposure of 4-HPR and developed lipid-based formulations to enhance its exposure. Pharmacokinetic analysis showed that clinical formulation achieved effective plasma concentrations, while novel formulations achieved more than 3-fold improvement. However, solubility and first-pass intestinal elimination limited the exposure, which could be improved through CYP inhibition. Simulated exposure profiles suggested that b.i.d dosage regime with clinical formulation and new formulations/CYP inhibition were viable options to increase exposure.
Article
Food Science & Technology
Jiaqiang Luo, Xinwei Ruan, Ching-Seng Ang, Yada Nolvachai, Philip J. Marriott, Pangzhen Zhang, Kate Howell
Summary: The preferences of consumers for different flavours and aromas in wine may be explained by factors such as cultural background, wine education, and personal taste. Saliva and wine interactions play a role in shaping the taste of wine, leading to different preferences among wine tasters. Specifically, the composition of proteins in saliva was found to influence wine perception and preference.
NPJ SCIENCE OF FOOD
(2023)
Article
Chemistry, Multidisciplinary
Fanmengjing Wang, David Pizzi, Yizhihao Lu, Kaiqiang He, Kristofer J. J. Thurecht, Matthew R. R. Hill, Philip J. J. Marriott, Mark M. M. Banaszak Holl, Kristian Kempe, Huanting Wang
Summary: Chiral separation membranes have great potential for separating racemic mixtures into enantiopure components, but fabricating scalable membranes with both high selectivity and flux is challenging. In this study, a new type of enantioselective membrane was prepared using enantiopure S-poly(2,4-dimethyl-2-oxazoline) macromonomers and supported by graphene oxide nanosheets. The S-PdMeOx-based membrane showed near-quantitative enantiomeric excess and a high flux. This work demonstrates the potential of homochiral polymers in chiral discrimination and provides a new route to develop highly efficient enantioselective membranes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
