Article
Neurosciences
Viktoria S. Pendeliuk, Igor V. Melnick
Summary: Synchronization of hippocampal interneurons (INs) relies on local cell interactions and intensity of network activity. In baseline conditions, a significant portion of inhibitory postsynaptic currents coincided between cells. Network activity induced excitatory and inhibitory events, with excitatory events capable of synchronizing IN firing. Glutamatergic mechanisms play a crucial role in initiating and dominating IN synchronization.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Tapojyoti Das, Meraj Ramezani, David Snead, Cristian Follmer, Peter Chung, Ka Yee Lee, David A. A. Holowka, Barbara A. A. Baird, David Eliezer
Summary: Alpha-synuclein plays an important role in regulating synaptic vesicle cycling, exerting both inhibitory and potentiating effects on vesicle release. The binding affinity of alpha-synuclein to isolated vesicles is a key determinant of its ability to potentiate release.
Article
Anesthesiology
Iris A. Speigel, Hugh C. Hemmings
Summary: The study reveals that isoflurane inhibits synaptic vesicle exocytosis from hippocampal glutamatergic neurones and GABAergic interneurones in a cell-type-specific manner depending on their expression of voltage-gated sodium channel subtypes.
BRITISH JOURNAL OF ANAESTHESIA
(2021)
Article
Nanoscience & Nanotechnology
Jiapei Yang, Li Xu, Ling Di, Yue Su, Xinyuan Zhu
Summary: PEG, as the gold standard for stealth polymer materials, is widely used in drug delivery due to its excellent properties. However, the lack of understanding about the fate of PEG at the cellular level has hindered its application in diagnosis and therapy. Research showed that PEG could be internalized into cells in 1 hour, localized to lysosomes, cytosol, ER, and mitochondria. The fate of PEG in cells could be regulated by conjugating different small molecules, which is crucial for the rational design of PEGylation for drug delivery.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Emily M. Grasso, Mayu S. Terakawa, Alex L. Lai, Ying Xue Xie, Trudy F. Ramlall, Jack H. Freed, David Eliezer
Summary: Complexins are important regulators of SNARE-mediated exocytosis of synaptic vesicles. The structural and functional characterization of mCpx1 C-terminal domain (CTD) reveals differences in inhibitory function compared to the worm counterpart wCpx1 CTD. The mCpx1 CTD shows structural divergences in regions critical for inhibiting spontaneous fusion of synaptic vesicles, suggesting a potential basis for evolutionary divergences in complexin function.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Neurosciences
Junhong Wu, Hui Zhang, Liu Yang, Yuanyuan Chen, Jiyuan Li, Min Yang, Xiaogang Zhang, Changlong He, Xuefeng Wang, Xin Xu
Summary: This study investigates the role of STX7 in epilepsy and finds that reduced expression of STX7 in the epileptic brain is associated with increased susceptibility to epileptic seizures. Overexpression of STX7, on the other hand, decreases susceptibility to seizures and alleviates epileptic activity. It is also found that STX7 affects the release of inhibitory neurotransmitters, but not the intrinsic excitability of neurons.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Plant Sciences
Martiniano Maria Ricardi, Niklas Wallmeroth, Cecilia Cermesoni, Dietmar Gerald Mehlhorn, Sandra Richter, Lei Zhang, Josephine Mittendorf, Ingeborg Godehardt, Kenneth Wayne Berendzen, Edda von Roepenack-Lahaye, York-Dieter Stierhof, Volker Lipka, Gerd Juergens, Christopher Grefen
Summary: The final step in secretion is membrane fusion facilitated by SNARE proteins that reside in opposite membranes. The formation of a trans-SNARE complex between one R and three Q coiled-coiled SNARE domains drives the final approach of the membranes providing the mechanical energy for fusion. Biological control of this mechanism is exerted by additional domains within some SNAREs.
Review
Plant Sciences
Xiaobo Zhu, Junjie Yin, Hongming Guo, Yuping Wang, Bingtian Ma
Summary: The vesicle trafficking apparatus is crucial for maintaining the homeostasis of membrane-enclosed organelles in eukaryotic cells. Research on vesicle trafficking in plants, particularly rice, is limited, and further investigations are needed to uncover the mysteries of this system.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Lennart Brodin, Dragomir Milovanovic, Silvio O. Rizzoli, Oleg Shupliakov
Summary: This review discusses recent data suggesting the protein alpha-synuclein as a component of the synaptic vesicle liquid phase, and considers possible implications of these data for disease.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Monica C. Quinones-Frias, J. Troy Littleton
Summary: The SYT family of proteins play crucial roles in regulating membrane trafficking at neuronal synapses, participating in synchronous and asynchronous fusion of synaptic vesicles and preventing spontaneous release. Changes in SYT isoforms can alter the fusion of synaptic vesicles and regulate trafficking of other subcellular organelles. However, the exact mechanisms by which SYTs interact with lipids and other effectors are still under investigation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Cell Biology
I. -Ying Kuo, Chih-Hsiung Hsieh, Wan-Ting Kuo, Chih-Peng Chang, Yi-Ching Wang
Summary: Cells in the tumor microenvironment require a class of checkpoints to regulate various secretion and transport processes. Recent studies have shown that Rab proteins mediate communication between diverse cargoes and substrates through vesicular trafficking and secretion pathways in the tumor microenvironment. This review focuses on the role and regulation of vesicular secretion pathways in the tumor microenvironment, and describes the latest research advances and future directions.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Zuodong Ye, Dawei Wang, Yingying Lu, Yunjiao He, Jingting Yu, Wenjie Wei, Chang Chen, Rui Wang, Liang Zhang, Liangren Zhang, Minh T. N. Le, William C. Cho, Mengsu Yang, Hongmin Zhang, Jianbo Yue
Summary: V1 shows significant anti-metastatic activity by inhibiting cancer cell migration and metastasis in vitro and in vivo, as well as suppressing colony formation, migration, and invasion of cancer cells. V1 targets CapZ beta to inhibit endosomal trafficking and metastasis processes.
Article
Biochemistry & Molecular Biology
Daniela Anni, Eva-Maria Weiss, Debarpan Guhathakurta, Yagiz Enes Akdas, Julia Klueva, Stefanie Zeitler, Maria Andres-Alonso, Tobias Huth, Anna Fejtova
Summary: The study shows that Aβ 1-42 and Aβ 1-16 can increase the size of the recycling pool of synaptic vesicles, driven by enhanced endogenous cholinergic signaling.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Neurosciences
Soulmee Koh, Wongyoung Lee, Sang Myun Park, Sung Hyun Kim
Summary: The study demonstrates the important role of Cav1 in synaptic vesicle exocytosis, with its knockdown significantly impairing synaptic vesicle function. Neurons rescued by triple mutants lacking palmitoylation sites of Cav1 show impairments in both synaptic transmission and retrieval, highlighting the importance of Cav1 palmitoylation in activity-driven synaptic vesicle dynamics.
Article
Neurosciences
Marcin Wyroslak, Grzegorz Dobrzanski, Jerzy W. Mozrzymas
Summary: In this study, we investigated the effect of NMDA-induced plasticity on tonic inhibition in somatostatin- and parvalbumin-containing interneurons. Our results showed that NMDA application enhanced tonic conductance in somatostatin-containing interneurons, while reducing tonic inhibition in both fast-spiking and non-fast-spiking parvalbumin-positive cells. These changes were associated with alterations in the content of α5 and δ-subunit-containing GABA(A) receptors.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Neurosciences
Ken Berglund, Lei Wen, Robert L. Dunbar, Guoping Feng, George J. Augustine
JOURNAL OF NEUROSCIENCE
(2016)
Article
Biology
Elina Nagaeva, Ivan Zubarev, Carolina Bengtsson Gonzales, Mikko Forss, Kasra Nikouei, Elena de Miguel, Lauri Elsila, Anni-Maija Linden, Jens Hjerling-Leffler, George J. Augustine, Esa R. Korpi
Article
Neurosciences
Martin Graf, Aditya Nair, Kelly L. L. Wong, Yanxia Tang, George J. Augustine
Article
Biology
Hunter E. Halverson, Jinsook Kim, Andrei Khilkevich, Michael D. Mauk, George J. Augustine, Upinder Singh Bhalla
Summary: The study demonstrates that the feedback inhibitory circuit between cerebellar Purkinje cells and molecular layer interneurons plays a crucial role in regulating neuronal activity during learning processes and learning-related movements.
Article
Multidisciplinary Sciences
Aditya Nair, Yue Yang Teo, George J. Augustine, Martin Graf, Hee - Sup Shin
Summary: The cholinergic system in the basal forebrain has a crucial role in various behaviors such as attention and learning, by modifying the impact of noise in neural populations. The confounding release of acetylcholine (ACh) and GABA by cholinergic inputs to the claustrum, a structure involved in attention control, has opposing effects on the electrical activity of claustrum neurons projecting to cortical and subcortical targets. This cholinergic switching between subcircuits offers a potential mechanism for neurotransmitter corelease in behaviorally relevant computations.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)