Journal
CATALYSIS COMMUNICATIONS
Volume 72, Issue -, Pages 147-149Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.catcom.2015.09.025
Keywords
1,3-Dicholoro-2-propanol (S)-epichlorohydrin; Saturation mutagenesis; Halohydrin dehalogenase; Epoxide hydrolase; Cascade reaction
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Funding
- National Natural Science Foundation of China [21176224]
- National High Technology Research and Development Program of China [2012AA022201B]
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Enantioselective biotransformation of prochiral 1,3-DCP by halohydrin dehalogenases (HHDHs) is particularly attractive since 100% yield of chiral epichlorohydrin (ECH) may be obtained. HheC mutant (P175S/W249P) displayed greatly improved enantiomeric excess (ee) of (S)-ECH from 5% to 95.3% in the catalyzed dehalogenation of 1,3-DCP at pH 8.0. (S)-ECH was enantioselectively biotransformed from 40 mM 1,3-DCP with 92.3% ee and 93.2% yield at pH 10.0. To increase the ee of (S)-ECH, the catalysis was carried out using HheC mutant coupled with epoxide hydrolase mutant and the maximum yield and ee of (S)-ECH reached 91.2% and >99%. (C) 2015 Elsevier B.V. All rights reserved.
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