Journal
JOURNAL OF NEUROIMMUNOLOGY
Volume 301, Issue -, Pages 7-11Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneuroim.2016.10.015
Keywords
Multiple sclerosis (MS); Experimental autoimmune encephalomyelitis (EAE); Remyelination; In vivo metabolic labeling; Quantitative colocalization analysis; Glatiramer acetate (GA)
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Funding
- Teva Pharmaceuticals [1575]
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The lack of biomarkers is a major obstacle for investigating myelin repair. We used metabolic incorporation of the choline analog - propargyl-choline (P-Cho) to label and visualize newly synthesized myelin in the CNS of mice induced with experimental autoimmune encephalomyelitis (EAE). We further developed unbiased colocalization analysis to quantify P-Cho incorporation specifically into the myelin. Our findings indicate that P-Cho injection to mice recovering from EAE, either spontaneously or following glatiramer acetate treatment, results in significant elevation of its incorporation into the myelin, offering a novel strategy for assessing remyelination in animal models and the remyelination potential of candidate drugs. (C) 2016 Elsevier B.V. All rights reserved.
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