Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume 16, Issue 6, Pages 5569-5576Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2016.11735
Keywords
PLGA; Nanoparticles; Fluorescence; Magnetic Resonance Imaging; Dual-Modal Imaging
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Funding
- Natural Science Foundation of China [21174130, 21374101]
- Marie Curie project [PIRSES-GA-2013-612673]
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Here, a novel poly(lactic-co-glycolic acid) (PLGA)-based nanoparticles (NPs) for magnetic resonance (MR) and fluorescence imaging was developed for cell imaging. PLGA NPs loaded with fluorescent dye Nile red (NR) and surface-coated with poly(ethyleneimine) (PEI) were produced in a single step nanoprecipitation process. Diethylenetriamine pentaacetic dianhydride (DTPA) was conjugated to PLGA/NR@PEI NPs through amidation reaction between -COOH of DTPA and -NH2 of PEI, which can chelate gadolinium (Gd3+) as an MR imaging contrast agent. The PLGA/NR@PEI-DTPA-Gd NPs exhibited a uniform particle size of similar to 200 nm and were stable in culture medium. These NPs had a high T-1 relaxivity (R-1) of 28.36 mM(-1) S-1. They did not introduce serious cytotoxicity against A549 lung cancer cells. Furthermore, fluorescence and MR imaging studies on A549 lung cancer cells in vitro revealed that PLGA/NR@PEI-DTPA-Gd NPs can serve as an efficient fluorescence/MR dual-modality imaging nanoprobe.
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