Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume 16, Issue 3, Pages 2296-2300Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2016.10933
Keywords
DNA Isolation and Purification; Magnetic Nanoparticle; Escherichia Coli O157:H7; Ultraviolet Spectrophotometer
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Funding
- Special Fund for Agro-scientific Research in the Public Interest [201303045]
- Chinese Universities Scientific Fund [2014RC013]
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The objective of this study was to evaluate the performance of a nucleic acid isolation and purification instrument using Escherichia coli O157:H7 as the model. The instrument was developed with magnetic nanoparticles for efficiently capturing nucleic acids and an intelligent mechanical unit for automatically performing the whole nucleic acid extraction process. A commercial DNA extraction kit from Huier Nano Company was used as reference. Nucleic acids in 1 ml of E. coli O157:H7 at a concentration of 5x10(8) CFU/mL were extracted by using this instrument and the kit in parallel and then detected by an ultraviolet spectrophotometer to obtain A260 values and A260/A280 values for the determination of the extracted DNA's quantity and purity, respectively. The A260 values for the instrument and the kit were 0.78 and 0.61, respectively, and the A260/A280 values were 1.98 and 1.93. The coefficient of variations of these parallel tests ranged from 10.5% to 16.7%. The results indicated that this nucleic acid isolation and purification instrument could extract a comparable level of nucleic acid within 50 min compared to the commercial DNA extraction kit.
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