Article
Biochemistry & Molecular Biology
Nan Zhou, Zhao Xu, Xiang Li, Sen Ren, Jing Chen, Hewei Xiong, Cheng Wang, Jiahe Guo, Yu Kang, Zhenbing Chen, Wenqing Li, Xiaofan Yang, Xing Zhang, Xiang Xu
Summary: This study aimed to induce the differentiation of ADSCs into SCs using SC-Exos and investigate the mechanisms involved through miRNA sequencing. The results showed that SC-Exos were effective in inducing ADSCs differentiation, with differentially expressed miRNAs playing a vital role.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Cell Biology
Silvan Klein, Andreas Siegmund, Andreas Eigenberger, Valerie Hartmann, Felix Langewost, Nicolas Hammer, Alexandra Anker, Konstantin Klein, Christian Morsczeck, Lukas Prantl, Oliver Felthaus
Summary: The study investigated three differentiation protocols for differentiating rat ASCs into specialized SC phenotypes, comparing their expressions of neurotrophins and myelin markers. The results showed diverse expression profiles of differentiated ASCs, with different protocols inducing varying levels of expression of neurotrophins and myelin markers.
Article
Cell & Tissue Engineering
Stefania Brambilla, Martino Guiotto, Enrica Torretta, Ilaria Armenia, Matteo Moretti, Cecilia Gelfi, Silvia Palombella, Pietro G. di Summa
Summary: Trauma-associated peripheral nerve injury is a common problem that contributes to sensory and motor disabilities. Schwann cells and adipose-derived stem cells (ASCs) can promote nerve regeneration, mainly by secreting neurotrophic factors. This study compared the neurotrophic properties of differentiated SC-like ASCs and HPL-cultured ASCs, and found that HPL-ASCs exhibited higher neurotrophic abilities.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell & Tissue Engineering
Shuhai Chen, Tetsuya Ikemoto, Takuya Tokunaga, Shouhei Okikawa, Katsuki Miyazaki, Shinichiro Yamada, Yu Saito, Yuji Morine, Mitsuo Shimada
Summary: This study successfully generated 3D SLC spheroids with improved functionality by modifying the differentiation protocol of ADSCs. The results demonstrated that these 3D SLC spheroids could more effectively promote nerve regeneration and have great potential for clinical treatment.
CELL TRANSPLANTATION
(2022)
Article
Pharmacology & Pharmacy
Guangyu Long, Quanhe Wang, Shaolin Li, Junzhong Tao, Boyan Li, Xiangxiang Zhang, Xi Zhao
Summary: The treatment utilizing adipose-derived stem cells supports new methods for promoting acute myocardial infarction in alginate/silk sericin lamellar-coated systems. The developed ADSC-ASS@L injectable hydrogels showed higher cell survival rates and pro-angiogenic and pro-inflammatory expression factors in vitro examinations, demonstrating favorable effects for successful heart damage therapy.
Review
Biochemistry & Molecular Biology
Anna Trzyna, Agnieszka Banas-Zabczyk
Summary: The secretome of ASCs, including exosomes, contains a variety of cytokines and non-coding RNAs that have therapeutic potential in a wide range of diseases. Utilizing ASC secretome as a cell-free therapy shows promise in overcoming limitations of cell-based therapies and improving treatment efficacy.
Article
Chemistry, Inorganic & Nuclear
Reihaneh Seyedebrahimi, Shahnaz Razavi, Jaleh Varshosaz, Elham Vatankhah, Mohammad Kazemi
Summary: This study suggests a potential method for inducing the differentiation of h-ADSCs into SCs by tuning the surface of aligned poly (lactic-co-glycolic acid) (PLGA) nanofibers to enhance attachment and proliferation of h-ADSCs on the scaffold. Additionally, the presence of BDNF and AuNPs on scaffold significantly improved the expression of SC markers compared to the control group.
JOURNAL OF CLUSTER SCIENCE
(2021)
Article
Cell Biology
Tomoko Ishikura, Hideaki Shiga, Yuka Nakamura, Takako Kanitani, Yasuhito Ishigaki, Takaki Miwa
Summary: This study aimed to determine whether nasally administered murine adipose-derived stem cells (ADSCs) could support olfactory regeneration in vivo. The results showed that ADSCs can promote the repair of olfactory epithelium damage and enhance olfactory perception. This finding indicates that nasally administered ADSCs secreting neurotrophic factors can stimulate the regeneration of olfactory epithelium, thereby improving odor aversion behavior in vivo.
Article
Biochemistry & Molecular Biology
Gang Yin, Bing Yu, Caiyue Liu, Yaofa Lin, Zheng Xie, Yiping Hu, Haodong Lin
Summary: Research has shown that adipose-derived stem cell exosomes (ADSC-Exos) promote the regeneration of the myelin sheath by inhibiting Schwann cells autophagy through miRNA-26b down-regulation of Kpna2.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhong Huang, Rebecca Powell, Svenja Kankowski, James B. Phillips, Kirsten Haastert-Talini
Summary: Researchers have successfully generated Schwann cells from human induced pluripotent stem cells by modifying existing protocols, resulting in sufficient cell numbers. They also found that specific coating and glucose levels are crucial for improving the differentiation process and increasing the yield of viable cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Siti Syahira Airuddin, Ahmad Sukari Halim, Wan Azman Wan Sulaiman, Ramlah Kadir, Nur Azida Mohd Nasir
Summary: Stem cells, particularly adipose-derived stem cells, have shown promising potential in disease treatment due to their differentiation abilities and secretion of growth factors. However, more research is needed to fully understand the safety and effectiveness of adipose-derived stem cells in medical treatment.
Article
Cell Biology
Jian Du, Zihui Wang, Xiao Liu, Cecilia Hu, Kevin J. J. Yarema, Xiaofeng Jia
Summary: In this study, metabolic glycoengineering technology is used to enhance the differentiation of adipose-derived stem cells (ASCs) into Schwann cells (SCs). This improvement can potentially contribute to neuronal regeneration and the future use of ASCs in regenerative medicine.
Article
Medicine, Research & Experimental
Giti Zarinfard, Maryam Aliakbari, Vajihe Asgari, Shahnaz Razavi
Summary: This study assessed the transdifferentiation potency of human adipose-derived stem cells into Schwann-like cells (SLCs) under triiodothyronine (T3) hormone treatment. The results showed that T3 administration significantly increased the differentiation and myelination potency of SLCs. Further experiments are needed to confirm the advantages of using a combination of autologous SLCs and T3 hormone for peripheral nerve injury recovery.
INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE
(2022)
Article
Developmental Biology
Aya Mikdache, Marie-Jose Bouied, Emilie Lesport, Brigitte Delespierre, Julien Loisel-Duwattez, Cindy Degerny, Marcel Tawk
Summary: This study demonstrates the crucial role of timely division of Schwann cells in mediating laminin expression to orchestrate radial sorting and peripheral myelination.
Article
Neurosciences
Xiangyun Yao, Zhiwen Yan, Xiaojing Li, Yanhao Li, Yuanming Ouyang, Cunyi Fan
Summary: Peripheral nerve injuries are common traumatic injuries worldwide, and the use of FK506-stimulated ADSCs has shown potential in promoting nerve regeneration, offering hope for the treatment of severe PNIs.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Materials Science, Multidisciplinary
Ali Honarvar, Saeed Karbasi, Batool Hashemibeni, Mohsen Setayeshmehr, Mohammad Kazemi, Ali Valiani
Summary: The methods of scaffold fabrication play a crucial role in cartilage tissue engineering, with extracellular matrix derivatives being utilized to improve chondrogenesis and cartilage regeneration. In this study, polycaprolactone scaffolds were prepared using 3D-printing and salt-leaching methods. Coating the scaffolds with fibrin and different concentrations of acellular solubilised ECM showed varying effects on porosity, water uptake, and cell viability. The most suitable concentration for acellular solubilized ECM to fabricate biocompatible PCL/fibrin/ECM scaffolds in cartilage tissue engineering was determined to be 2%.
MATERIALS TECHNOLOGY
(2022)
Article
Medical Laboratory Technology
Zeinab Abdollahi, Mohammad Amin Tabatabaiefar, Mahnaz Noruzi, Paniz Miar, Mohammad Kazemi, Azar Naimi, Mohammad Hasan Emami, Mehrdad Zeinalian
Summary: This study evaluated BAT34c4 and BAT26 mononucleotide markers as a cost-effective panel for MSI testing, comparing it with the Promega kit. The results showed that the novel 2-marker kit has similar accuracy as the Promega kit, but at a lower cost.
LABORATORY MEDICINE
(2022)
Correction
Parasitology
Azadeh Hasanzadeh, Abdollah Rafiei, Mohammad Kazemi, Molouk Beiromvand, Amin Bahreini, Hossein Khanahmad
ACTA PARASITOLOGICA
(2022)
Article
Parasitology
Azadeh Hasanzadeh, Abdollah Rafiei, Mohammad Kazemi, Molouk Beiromvand, Amin Bahreini, Hossein Khanahmad
Summary: This study revealed the significant overexpression of TIMP mRNAs in the fibrotic liver tissue of patients with cystic echinococcus, possibly due to increased expression of MMPs in the peri-cystic tissue. However, no significant difference in TIMP-1 levels before and after surgery was found, which may be attributed to recurrence or heterogeneity in cyst type.
ACTA PARASITOLOGICA
(2022)
Article
Biochemistry & Molecular Biology
Farshad Amiri, Melika Babaei, Nima Jamshidi, Maria Agheb, Mohammad Rafienia, Mohammad Kazemi
Summary: The study investigated the structural and biological properties of 3D-printed polyurethane scaffolds coated with gellan gum, hyaluronic acid, and glucosamine. The surface coating improved cell behavior, increased expression levels of collagen II and aggrecan genes, and enhanced the scaffold's mechanical and degradation properties, making it a promising candidate for meniscus tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Medicine, Research & Experimental
Ali Rajool Dezfuly, Azadeh Safaee, Noushin Amirpour, Mohammad Kazemi, Alireza Ramezani, Morteza Jafarinia, Alireza Dehghani, Hossein Salehi
Summary: This study compared the therapeutic effects of human adipose mesenchymal stem cells (hADSCs) and their secretome on an in vivo model of sodium iodate retinal neurodegeneration. The results showed that the secretome of hADSCs had significant therapeutic effects on retinal cells, particularly in terms of photoreceptor regeneration and retinal function. These findings can be helpful in designing more precise treatments for different neurodegenerative diseases.
Article
Sport Sciences
Nakisa Soltani, Sayyed Mohammad Marandi, Volga Hovsepian, Mohammad Kazemi, Nafiseh Esmaeil
Summary: This study compared the effects of different types of high-intensity interval training (HIIT) on meta-inflammation during obesity. The results showed that resistance exercise training can significantly decrease the levels of TLR4, TRIF, IRF3, TNF alpha, and IFN gamma, enhancing the immunomodulatory adaptations to HIIT.
EUROPEAN JOURNAL OF SPORT SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Akram Sharifian, Jaleh Varshosaz, Mehdi Aliomrani, Mohammad Kazemi
Summary: The study investigated the effect of nasal spray of polydopamine coated micelles loaded with ibudilast on brain targeted delivery and effectiveness in remyelination and neuroprotection in animal model of multiple sclerosis. The results showed that the polydopamine coated micelles significantly increased myelin fiber percentage and expression of Mbp, Olig2 and Mog genes in the corpus callosum. These findings suggest that polydopamine coated micelles loaded with ibudilast have potential as an anti-inflammatory and neuroprotective agent in active multiple sclerosis.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Genetics & Heredity
Maryam Hosseini, Behnaz Hammami, Mohammad Kazemi
Summary: This study aimed to identify potential diagnostic and therapeutic targets for Endometriosis (EMs) by constructing a regulatory network and using machine learning approaches. A total of 119 differentially expressed genes (DEGs) were identified, and a regulatory network consisting of 52 mRNAs, 249 miRNAs, and 37 TFs was constructed. A diagnostic model using hub genes from the network achieved high accuracy (AUC of 0.98 and 0.92 in training and validation cohorts, respectively). Docking analysis identified two compounds (rofecoxib and retinoic acid) with potential therapeutic effects on EMs.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Obstetrics & Gynecology
Mahboobeh Golchin, Mohammad Kazemi, Mohammadreza Sharifi, Reza Mohammadzadeh
Summary: This study aimed to explore the targeting effect of oncogenic lncRNA SIRLNT, which is significantly upregulated in human breast cancer cells. By using LNA GapmeRs antisense oligonucleotides to knock down lncRNA SIRLNT expression, it was found that inhibiting lncRNA SIRLNT could suppress cell proliferation, induce apoptosis and necrosis in MCF-7 cell line, and upregulate SIRT1 gene (a molecule influenced by lncRNA SIRLNT). Therefore, inhibition of lncRNA SIRLNT could be a promising therapeutic strategy to prevent the progression of breast cancer.
INDIAN JOURNAL OF GYNECOLOGIC ONCOLOGY
(2023)
Article
Chemistry, Medicinal
Ahmad Teimourinejad, Batool Hashemibeni, Hossein Salehi, Fateme Sadat Mostafavi, Mohammad Kazemi, Hamid Bahramian
Summary: This study aimed to investigate the capability of Pomegranate Fruit hydroalchoholic Extract (PFE) in differentiating human adipose derived stem cells (hASCs) into chondrocytes on fibrin scaffold. The results showed that PFE was effective in chondrogenic induction. Further studies are needed to understand the process of chondrogenic induction using PFE.
AVICENNA JOURNAL OF PHYTOMEDICINE
(2023)
Article
Medicine, General & Internal
Mohammad Abdolvand, Minoosh Sadeghi, Mohammad Emami, Alireza Fahim, Hojjatolah Rahimi, Elham Amjadi, Abdolmehdi Baghaei, Shirin Abdolvand, Fatemeh Maghool, Sara Feizbakhshan, Sharareh Salmanizadeh, Ehsan Heidari, Mohammad Chehelgerdi, Mahsa Khodadoostan, Maryam Ebrahim, Faeze Beni, Mohammad Kazemi, Simin Hemati, Farinaz Khosravian, Hassan Rahimi, Alireza Samadian, Mansoor Salehi
Summary: Long noncoding RNAs (lncRNAs) have been identified as important modulatory molecules in various cancers, including colorectal cancer (CRC). In this study, the researchers aimed to identify potential RNA biomarkers and construct a novel competing endogenous RNA (ceRNA) network in CRC. Through bioinformatics methods and experimental validation, they discovered upregulation of KCNQ1OT1, NR3C1, and XIAP, as well as downregulation of miR-421. The ceRNA network consisted of 17 lncRNAs, 2 mRNAs, and 42 miRNAs, revealing potential interactions between KCNQ1OT1, miR-421, NR3C1, and XIAP.
JOURNAL OF RESEARCH IN MEDICAL SCIENCES
(2022)
Article
Medicine, Research & Experimental
Paniz Miar, Mohammad Tabatabaiefar, Zeinab Abdollahi, Mahnaz Noruzi, Mohammad Kazemi, Azar Naimi, Mohammad Emami, Shahrokh Izadi, Mehrdad Zeinalian
Summary: In this pilot study, the diagnostic value of a novel triplex panel including three mononucleotide markers in colorectal cancer patients has been evaluated. The results showed that this panel has high sensitivity and specificity, suggesting its potential as a diagnostic tool for colorectal cancer.
ADVANCED BIOMEDICAL RESEARCH
(2022)
Article
Chemistry, Medicinal
Nahid Moradi-Gharibvand, Mohsen Setayeshmehr, Mohammad Kazemi, Azadeh Safaee, Laya Sadat Khorsandi, Darioush Bijan Nejad, Seyed Javed Hasheminia, Batool Hashemibeni
Summary: This study investigates the effect of pomegranate seed extract (PSE) on the cell cycle and apoptosis of the MCF-7 cell line in co-culture conditions with adipose-derived stem cells (ADSCs). The results show that ADSCs reduce cell viability and proliferation of MCF-7 cells, and adding PSE to the medium increases the apoptosis of cancer cells.
RESEARCH IN PHARMACEUTICAL SCIENCES
(2022)
Article
Public, Environmental & Occupational Health
Seyyed R. Mousavi, Hajie Lotfi, Sharareh Salmanizadeh, Sara Feizbakhshan, Farinaz Khosravian, Maryam S. Sajjadi, Sajad R. Komachali, Faeze A. Beni, Banafshe Torkan, Mohammad Kazemi, Ramin Sami, Mansoor Salehi
Summary: This study analyzed two datasets of gene expression in SARS and H1N1 patients, indicating that antibiotics may influence the immune response of COVID-19 patients by modulating the expression of neutrophil-related genes.
HEALTH SCIENCE REPORTS
(2022)