Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 428, Issue 5, Pages 889-892Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2015.12.022
Keywords
biotechnology; computational design; information encoding; regulatory RNA; synthetic biology
Categories
Funding
- Spanish Ministry of Economy [AGL2013-49919-EXP]
- CSIC Intramural [201440I017]
- EPSRC-BBSRC [BB/M017982/1]
- School of Life Sciences
- [FP7-ICT-FET-610730]
- [FP7-KBBE-613745]
- Biotechnology and Biological Sciences Research Council [BB/M017982/1] Funding Source: researchfish
- BBSRC [BB/M017982/1] Funding Source: UKRI
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In order to explore the possibility of adding new functions to preexisting genes, we considered a framework of riboregulation. We created a new riboregulator consisting of the reverse complement of a known riboregulator. Using computational design, we engineered a cis-repressing 5' untranslated region that can be activated by this new riboregulator. As a result, both RNAs can orthogonally trans-activate translation of their cognate, independent targets. The two riboregulators can also repress each other by antisense interaction, although not symmetrically. Our work highlights that antisense small RNAs can work as regulatory agents beyond the antisense paradigm and that, hence, they could be interfaced with other circuits used in synthetic biology. (C) 2016 The Authors. Published by Elsevier Ltd.
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