A global meta-analysis of woody plant responses to elevated CO2: implications on biomass, growth, leaf N content, photosynthesis and water relations

Background Atmospheric CO2 may double by the year 2100, thereby altering plant growth, photosynthesis, leaf nutrient contents and water relations. Specifically, atmospheric CO2 is currently 50% higher than pre-industrial levels and is projected to rise as high as 936 mu mol mol(-1) under worst-case scenario in 2100. The objective of the study was to investigate the effects of elevated CO2 on woody plant growth, production, photosynthetic characteristics, leaf N and water relations. Methods A meta-analysis of 611 observations from 100 peer-reviewed articles published from 1985 to 2021 was conducted. We selected articles in which elevated CO2 and ambient CO2 range from 600-1000 and 300-400 mu mol mol(-1), respectively. Elevated CO2 was categorized into < 700, 700 and > 700 mu mol mol(-1) concentrations. Results Total biomass increased similarly across the three elevated CO2 concentrations, with leguminous trees (LTs) investing more biomass to shoot, whereas non-leguminous trees (NLTs) invested to root production. Leaf area index, shoot height, and light-saturated photosynthesis (A(max)) were unresponsive at < 700 mu mol mol(-1), but increased significantly at 700 and > 700 mu mol mol(-1). However, shoot biomass and A(max) acclimatized as the duration of woody plants exposure to elevated CO2 increased. Maximum rate of photosynthetic Rubisco carboxylation (V-cmax) and apparent maximum rate of photosynthetic electron transport (J(max)) were downregulated. Elevated CO2 reduced stomatal conductance (g(s)) by 32% on average and increased water use efficiency by 34, 43 and 63% for < 700, 700 and > 700 mu mol mol(-1), respectively. Leaf N content decreased two times more in NLTs than LTs growing at elevated CO2 than ambient CO2. Conclusions Our results suggest that woody plants will benefit from elevated CO2 through increased photosynthetic rate, productivity and improved water status, but the responses will vary by woody plant traits and length of exposure to elevated CO2.

Automated summary

Elevated CO2 concentration affects the growth, photosynthetic characteristics, nitrogen content, and water relations of woody plants. Woody plants show increased photosynthetic rate, productivity, and improved water status under elevated CO2 conditions, but these responses vary depending on woody plant traits and the duration of exposure to elevated CO2.