Journal
JOURNAL OF MICROENCAPSULATION
Volume 33, Issue 1, Pages 80-88Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/02652048.2015.1134686
Keywords
Cell microencapsulation; CHO-DP12; alginate; animal cell culture; mechanical resistance; perfusion culture
Funding
- Irish Research Council (IRC)
- National Institute of Biotechnology Research and Training (NIBRT)
- European Regional Development Fund (ERDF), part of the European Union Structural Funds Programme
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Microcapsules for high cell density culture of mammalian cells have found an increasing interest, however, the poor stability of the microcapsules and the lack of characterisation methods led to few quantitative results. Alginate-poly-L-lysine (PLL) microcapsules have been studied in detail in order to form a basis for comparison of capsules made from different polymers. Since the microcapsules can be easily retained in the bioreactor without the need for a cell separation device, high cell densities were achieved with a maximum of 4x10(7) cell/ml(microcapsules), corresponding to a colonisation of 5% of the internal capsule volume. Measurement of microcapsule integrity and mechanical resistance showed that alginate-PLL microcapsules are not suitable for perfusion cultures since they are very sensitive to media composition, mainly the presence of non-gelling ions that have a higher affinity for alginate than PLL and Ca2+, leading to the leakage of PLL and Ca2+, and to microcapsule rupture.
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