Journal
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 26, Issue 12, Pages 2066-2075Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1605.05057
Keywords
Chlamydomonas reinhardtii; lipid content; mutant; proteome
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Funding
- Golden Seed Project, Ministry of Oceans and Fisheries [213004-04-4-SBA30]
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [NRF-2015R1A2A2A01004733]
- Nuclear R&D program of the Ministry of Science, ICT & Future Planning (MSIP)
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In this study, an enhanced lipid-producing mutant strain of the microalga Chlamydomonas reinhardtii was developed by gamma irradiation. To induce the mutation, C. reinhardtii was gamma irradiated at a dose of 400 Gy. After irradiation, the surviving cells were stained with Nile red. The mutant (Cr-4013) accumulating 20% more lipid than the wild type was selected. Thin-layer chromatography revealed the triglyceride and free fatty acid contents to be markedly increased in Cr-4013. The major fatty acids identified were palmitic acid, oleic acid, linoleic acid, and linolenic acid. Random amplified polymeric DNA analysis showed partial genetic modifications in Cr-4013. To ascertain the changes of protein expression in the mutant strain, two-dimensional electrophoresis was conducted. These results showed that gamma radiation could be used for the development of efficient microalgal strains for lipid production.
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