Article
Chemistry, Analytical
Jirakrit Saetang, Phutthipong Sukkapat, Suriya Palamae, Prashant Singh, Deep Nithun Senathipathi, Jirayu Buatong, Soottawat Benjakul
Summary: A detection method combining multiplex PCR with lateral flow dipstick (LFD) assay was developed to detect pathogenic V. parahaemolyticus. The method showed high sensitivity and specificity and could be used in the food safety sector.
Article
Chemistry, Multidisciplinary
Yue Zhai, Xiangjun Meng, Li Li, Yushen Liu, Kun Xu, Chao Zhao, Juan Wang, Xiuling Song, Juan Li, Minghua Jin
Summary: This study presents a rapid and sensitive detection method for Vibrio parahaemolyticus by integrating magnetic nanobeads and quantum dots technology. The detection combines immunoseparation and immunofluorescence to achieve efficient detection of Vibrio parahaemolyticus, showing satisfactory results in real food samples.
Article
Food Science & Technology
Monica Campas, Sandra Leonardo, Ainoa Ferre-Gode, Asif A. Chowdhury, Anna Toldra, Karl B. Andree, Ana Roque
Summary: Vibrio parahaemolyticus is a dominant cause of foodborne infections and can accumulate in shellfish. A duplex electrochemical biosensor was developed to detect the tdh and trh genes of V. parahaemolyticus in oysters, providing a quick and specific method for monitoring this pathogen.
Article
Engineering, Environmental
Zaafrane Sami, Maatouk Kaouthar, Cherif Nadia, Ben Mansour Hedi
Summary: This study investigated the impact of sunlight and salinities on the survival of Vibrio parahaemolyticus strains. The results showed that sunlight exposure caused the strains to become non-culturable but viable. The tdh positive strains were more culturable than the non-virulent or trh positive strain. The survival time was longer at certain salinities and the non-pathogenic strain survived longer than the virulent ones. Resuscitation experiments did not revive the non-culturable bacteria. Microscope observations showed that the strains remained motile after losing their culturability.
WATER ENVIRONMENT RESEARCH
(2022)
Article
Microbiology
Abu Baker Siddique, M. Moniruzzaman, Sobur Ali, Md Nayem Dewan, Mohammad Rafiqul Islam, Md Shafiqul Islam, Mohammed Badrul Amin, Dinesh Mondal, Anowar Khasru Parvez, Zahid Hayat Mahmud
Summary: The study investigated the incidence and virulence potential of V. parahaemolyticus isolates in aquaculture farms in Bangladesh, revealing high antibiotic resistance and O8:KUT as the predominant serotype. Environmental factors such as temperature and salinity were significantly correlated with V. parahaemolyticus contamination. The genetic variation and relatedness among pathogenic isolates were determined using ERIC-PCR.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Mariam Almejhim, Mohammed Aljeldah, Nasreldin Elhadi
Summary: This study investigated the presence of toxigenic V. parahaemolyticus in coastal water in the Eastern Province of Saudi Arabia using IMS, chromogenic agar medium, and PCR. The results showed a high genomic diversity among the V. parahaemolyticus isolates, with some strains carrying toxin genes.
Article
Chemistry, Analytical
Ritong Sun, Jiao Chen, Yingeng Wang, Zheng Zhang, Yong Li, Fengmei Li, Cuiping Ma, Qingxia Han, Yanjing Shi
Summary: A highly sensitive and specific real-time narrow thermal-cycling amplification detection method based on accelerated strand exchange amplification (ASEA) was developed for the detection of Vibrio parahaemolyticus. The method can detect low concentrations of Vibrio parahaemolyticus in cultured samples and artificially spiked scallop meat within a short time. It is a useful tool for rapid detection of Vibrio parahaemolyticus in seafood samples.
ANALYTICAL METHODS
(2023)
Article
Fisheries
Tao Liu, Meijiao Wu, Yachen Tian, Xiaoting Liu, Cheng Liu, Qingqiang Xu, Qing Liu
Summary: A label-free and lac dye coloration-based LFIS method was developed for the rapid detection of V. parahaemolyticus in seafood. This method reduces restrictions on labelling materials and pairing of two antibodies, and shows high detection sensitivity.
JOURNAL OF FISH DISEASES
(2022)
Article
Microbiology
Zhuosheng Liu, Chao Liao, Luxin Wang
Summary: In this study, the population dynamics of Vibrio parahaemolyticus (Vp) strains with different virulence genes were characterized by inoculating them into seawater at temperatures mimicking different seasons. The transcriptomic responses of the strains containing the thermostable direct hemolysin (tdh+) gene and the tdh-related hemolysin (trh+) gene were investigated. The results showed that the tdh+ strain had a higher growth rate than the trh+ strain at 30 degrees C, while there was no difference between the two strains at 10 degrees C. The RNA sequencing results revealed specific metabolic changes in the trh+ strain at 30 degrees C and downregulation of histidine metabolism and biofilm formation pathways in both strains at 10 degrees C. The expression of virulence-associated genes did not differ significantly between the two temperatures.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Haibo Zhou, Xinmei Liu, Zhaoxin Lu, Antuo Hu, Wenjie Ma, Changzheng Shi, Xiaomei Bie, Yiyu Cheng, Haijing Wu, Jun Yang
Summary: A new method called PMA-duplex ddPCR was developed for the quantitative determination of viable Vibrio parahaemolyticus in aquatic products, with lower limit of detection and high accuracy. This method allows for the detection of V. parahaemolyticus without pre-enrichment.
Article
Immunology
Di Wang, Enliang Liu, Haiyang Liu, Xi Jin, Chunyan Niu, Yunhua Gao, Xiaofeng Su
Summary: In this study, a ddPCR assay was developed to detect and quantify V. nonalfalfae and V. albo-atrum, two pathogenic fungi causing Verticillium wilt. The performance of ddPCR was compared with qPCR, and ddPCR showed similar detection sensitivity, enhanced positive rate for low-abundance fungi, and superior diagnostic performance on field tissues. The quantification results of ddPCR were generally higher than qPCR, potentially due to differing amplification efficiencies. Overall, ddPCR method enables sensitive detection, accurate quantification, and improved precision for evaluating disease progression and enacting effective disease control.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Xueyun Chen, Liu Wang, Fang He, Ganghui Chen, Linlin Bai, Kaiyu He, Fang Zhang, Xiahong Xu
Summary: A novel label-free, colorimetric method for visual detection of V. parahaemolyticus was proposed, utilizing CRISPR/Cas12a to trim G-quadruplex DNAzyme and prevent color development of ABTS(2-). The method showed sensitivity comparable to real-time LAMP, detecting 6.1 x 10^2 CFU/mL V. parahaemolyticus in shrimp samples, making it a promising universal biosensing strategy for field pathogenic bacterial testing.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su
Summary: This study developed a droplet digital PCR (ddPCR) assay for accurate and sensitive detection and quantification of Verticillium dahliae and V. longisporum, showing that ddPCR is more sensitive and accurate than qPCR for quantifying trace amounts of these pathogens in field samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Ruojun Mu, Chengchu Liu, Salina Parveen, Donald Webster, Jie Pang
Summary: This study demonstrated that salinity and temperature have remarkable effects on purging Vibrio parahaemolyticus from oysters. Under specific conditions, such as 20 ppt salinity and 10 degrees Celsius temperature combined with a pre-chilled system, a significant reduction in Vibrio parahaemolyticus levels can be achieved by Day 7.
Article
Microbiology
Cristina Bacian, Cristobal Verdugo, Katherine Garcia, Josu Perez-Larruscain, Ignacio de Blas, Viviana Cachicas, Carmen Lopez-Joven
Summary: Vibrio parahaemolyticus is the leading cause of seafood-associated bacterial gastroenteritis worldwide. This study determined the prevalence of V. parahaemolyticus in mussels from two natural growing areas in Chile, finding higher detection rates in the Valdivia River Estuary compared to the Reloncavi Fjord, with significant influence from seasonal water temperature and area factors.
FRONTIERS IN MICROBIOLOGY
(2021)