4.7 Article

Lateral Flow Immunoassay With Upconverting Nanoparticle-Based Detection for Indirect Measurement of Interferon Response by the Level of MxA

Journal

JOURNAL OF MEDICAL VIROLOGY
Volume 89, Issue 4, Pages 598-605

Publisher

WILEY-BLACKWELL
DOI: 10.1002/jmv.24689

Keywords

lateral flow; immunoassay; MxA; upconversion; whole-blood; interferon

Categories

Ask authors/readers for more resources

Myxovirus resistance protein A (MxA) is a biomarker of interferon-induced gene expression state involved in many viral infections and some autoimmune disorders. It has a variety of potential utilities in clinical diagnostics, including distinguishing between bacterial and viral infections. Currently, MxA-assays are used for monitoring of IFN-b therapy in multiple sclerosis (MS) patients. As a proof-of-concept for rapid quantitative measurement of interferon response, a lateral flow immunoassay (LFIA) with upconverting nanoparticle (UCNP) reporters was developed and evaluated with clinical whole blood samples to assess the potential for a rapid and user-friendly quantitative assay for MxA, since the currently available rapid test for MxA (FebriDX) produces only qualitative result. The high detection sensitivity enabled by the UCNP reporter technology allowed the sample pre-treatment with dilution of whole blood into lysis buffer at a detectable analyte concentration. The assay can be done within 2 hr and the results correlate with the reference MxA-ELISA, which requires an overnight incubation. With 36 samples, R-2 for linear regression was 0.86. The assay detected 96% of the IFN-b responders with 89% specificity using a cut-off level of 100 mu g/L for an elevated MxA-concentration. (C) 2016 Wiley Periodicals, Inc.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available