4.3 Article

Prevalence of Escherichia coli O157:H7 From House Flies (Diptera: Muscidae) and Dairy Samples in North Central Florida

Journal

JOURNAL OF MEDICAL ENTOMOLOGY
Volume 54, Issue 3, Pages 733-741

Publisher

OXFORD UNIV PRESS INC
DOI: 10.1093/jme/tjw205

Keywords

PCR; cattle manure; enteric bacteria; pathogen; molecular isolation

Funding

  1. University of Florida Agricultural Experiment Station federal formula funds from Cooperative State Research, Education, and Extension Service, U.S. Department of Agriculture [FLA-04598]

Ask authors/readers for more resources

Efficient detection of enterohemorrhagic Escherichia coli O157:H7 is important to monitor the safety of food products obtained from cattle, and it has been primarily accomplished by analyzing manure samples by selective cultivation techniques, PCR, and ELISA. As each technique suffers from different biases, there may be value in using multiple methods and samples to increase detection efficiency. Difficulties associated with cattle manure sampling can be circumvented by isolation of E. coli O157:H7 from house flies, Musca domestica (L.), which present as an important vector for spreading diseases. Thus, isolation of pathogens directly from house flies provides information about the potential human health impact that house fly dispersal can have because of pathogen distribution. House flies can disperse from dairy farms, where E. coli O157:H7 endemically thrive in cattle, to restaurants where food is prepared and served. Here, we report that detecting E. coli O157:H7 in house flies was 2.7 times more frequent than in manure from nearby dairy farms. Flies appear to offer a promising alternative in efforts to detect E. coli O157:H7 in dairy farms, restaurants, processing plants, and other establishments.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.3
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Entomology

Exposure Timing and Method Affect Beauveria bassiana (Hypocreales: Cordycipitaceae) Efficacy Against House Fly (Diptera: Muscidae) Larvae

Roxie L. White, Christopher J. Geden, Phillip E. Kaufman

Summary: The study showed that high concentrations of Beauveria bassiana had a significant inhibitory effect on first- and second-instar house fly larvae, but not on third-instar larvae. However, environmental factors such as temperature and medium composition did not significantly affect the efficacy of B. bassiana against house fly larvae.

JOURNAL OF MEDICAL ENTOMOLOGY (2021)

Article Agriculture, Multidisciplinary

A brief hot-water treatment alleviates chilling injury symptoms in fresh tomatoes

Francisco E. Loayza, Jeffrey K. Brecht, Amarat H. Simonne, Anne Plotto, Elizabeth A. Baldwin, Jinhe Bai, Elena Lon-Kan

Summary: HW treatment at 52 degrees C for 5 min significantly reduced chilling-associated decay and increased antioxidants in 'BHN-602' tomatoes stored at different temperatures, although it did not enhance sensory quality.

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2021)

Article Entomology

Prevalence and distribution of pathogen infection and permethrin resistance in tropical and temperate populations ofRhipicephalus sanguineuss.l. collected worldwide

N. S. G. Tucker, E. N. I. Weeks, L. Beati, P. E. Kaufman

Summary: This study tested populations of the brown dog tick from various regions globally to identify the distribution of resistance mechanisms, pathogen-vector interactions, and phylogeny in relation to latitude. The results shed light on the geographical distribution of known resistance mechanisms and provide insights into the interactions between pathogens and vectors.

MEDICAL AND VETERINARY ENTOMOLOGY (2021)

Article Entomology

Prevalence of Field-Collected House Flies and Stable Flies With Bacteria Displaying Cefotaxime and Multidrug Resistance

Matthew T. Pileggi, John R. Chase, Runhang Shu, Lin Teng, Kwangcheol C. Jeong, Phillip E. Kaufman, Adam C. N. Wong

Summary: Antibiotic use in livestock is a major driver of resistance evolution and spread, with a missing link between agricultural antibiotic use and its impact on human health. This study found that house flies and stable flies from a livestock operation carried multidrug-resistant bacteria, including those resistant to cefotaxime. The fly gut may serve as a reservoir for the acquisition and dissemination of resistance genes.

JOURNAL OF MEDICAL ENTOMOLOGY (2021)

Article Entomology

Adulticidal Efficacy and Sublethal Effects of Metofluthrin in Residual Insecticide Blends Against Wild Aedes albopictus (Diptera: Culicidae)

Christopher S. Bibbs, Phillip E. Kaufman, Rui-De Xue

Summary: Field development for optimizing the use patterns of spatial repellents is an ongoing research need. Results from investigating volatile pyrethroids in blends formulated into vegetation spray show that combination treatments have consistently higher adult mosquito reductions compared to single applications of metofluthrin or other existing products. Additionally, metofluthrin-containing treatments demonstrate significant decrease in nonviable mosquito eggs, total eggs, and adult mosquitoes, suggesting potential complex interactions for mosquito control in the field.

JOURNAL OF ECONOMIC ENTOMOLOGY (2021)

Article Entomology

Comparative Virulence of Metarhizium anisopliae and Four Strains of Beauveria bassiana Against House Fly (Diptera: Muscidae) Adults With Attempted Selection for Faster Mortality

Roxie L. White, Christopher J. Geden, Phillip E. Kaufman, Dana Johnson

Summary: This study compared the virulence of Metarhizium anisopliae and four Beauveria bassiana strains for managing house flies, finding that some strains killed flies faster than M. anisopliae. However, attempts to induce faster fly mortality through selection did not result in shorter time to fly death.

JOURNAL OF MEDICAL ENTOMOLOGY (2021)

Article Engineering, Manufacturing

Investigation of phenolic compounds and antioxidant activity in red and yellow onions and a synergistic utilization of skin extract in modified atmosphere packaging of salmon (Salmo salar)

Senem Guner, Ziynet Boz, Yavuz Yagiz, Zeynal Topalcengiz, Bruce A. Welt, Paul Sarnoski, Amarat Simonne, Hordur G. Kristinsson, Maurice R. Marshall

Summary: This study found that the red onion skin extract and modified atmosphere packaging have synergistic effects on lipid oxidation of salmon, increasing the antioxidant capacity and extending the shelf life of salmon.

PACKAGING TECHNOLOGY AND SCIENCE (2021)

Article Multidisciplinary Sciences

Color biogenesis data of tomatoes treated with hot-water and high temperature ethylene treatments

Francisco E. Loayza, Jeffrey K. Brecht, Amarat H. Simonne, Anne Plotto, Elizabeth A. Baldwin, Jinhe Bai, Elena Lon-Kan

Summary: Controlled postharvest stresses were used to induce the synthesis of carotenoids in tomato fruit and track the color changes from green to red. The study found a correlation between chloroplast disorganization and chromoplast biogenesis during the early developmental stages of tomato ripening, marked by a sigmoidal curve in color changes.

DATA IN BRIEF (2021)

Article Entomology

Evidence of Permethrin Resistance and Fipronil Tolerance in Rhipicephalus sanguineus s.l. (Acari: Ixodidae) Populations From Florida and California

Yuexun Tian, Caitlin E. Taylor, Cynthia C. Lord, Phillip E. Kaufman

Summary: In this study, the resistance expression of Rhipicephalus sanguineus s.l. strains to permethrin and fipronil was evaluated. The results showed high resistance to permethrin and tolerance to fipronil in both Florida and California populations. The Florida population utilized both metabolic resistance and target site insensitivity against permethrin, while the California population had the target-site insensitivity resistance allele.

JOURNAL OF MEDICAL ENTOMOLOGY (2023)

Article Biotechnology & Applied Microbiology

Potential key genes involved in metabolic resistance to malathion in the southern house mosquito, Culex quinquefasciatus, and functional validation of CYP325BC1 and CYP9M12 as candidate genes using RNA interference

Xinyue Huang, Phillip E. Kaufman, Giridhar N. Athrey, Chris Fredregill, Christina Alvarez, Vinaya Shetty, Michel A. Slotman

Summary: In this study, we found that detoxification enzyme genes, especially cytochrome P450 genes, play an important role in metabolic resistance to the insecticide malathion in Culex quinquefasciatus mosquitoes. Through RNA interference, we validated the functional roles of the CYP325BC1 and CYP9M12 genes in malathion resistance and demonstrated that knockdown of these genes significantly increased susceptibility to malathion in Culex quinquefasciatus.

BMC GENOMICS (2023)

Article Horticulture

Comparative Analysis of Qualitative Attributes for Selection of Calabaza Genotypes in the Southeast United States

Skylar R. Moreno, Masoud Yazdanpanah, Tianyi Huang, Charles A. Sims, Carlene A. Chase, Geoffrey Meru, Amarat Simonne, Andrew J. MacIntosh

Summary: This study aimed to determine and compare the quality parameters of novel calabaza germplasm lines with commercially available cultivars of calabaza and butternut squash. Promising germplasms, such as UFTP 8 and UFTP 24, showed high quality attributes including color saturation, degrees Brix, yeast fermentable extract, and firmness/hardness. These findings demonstrate the potential to enhance consumer preferences for calabaza through breeding and provide a basis for commercial release of superior germplasm.

HORTICULTURAE (2023)

Article Horticulture

Tocopherols, Phylloquinone, Ascorbic Acid, and Sugar Contents in Hydroponically Grown Lettuce

Jesse J. Murray, Scott R. Latimer, Amarat H. Simonne, Gilles J. Basset, Robert C. Hochmuth, German Sandoya

Summary: Growing vegetables in controlled environments is a growing industry in Florida and the US. Lettuce is a popular vegetable in the US and growing it in controlled environments near urban areas can reduce transportation time and degradation. Genetic variation for key vitamins in lettuce was found among different lettuce types, and selecting lettuce in a representative environment could result in cultivars with higher vitamin content.

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE (2023)

Article Entomology

House Fly (Diptera: Muscidae): Biology, Pest Status, Current Management Prospects, and Research Needs

C. J. Geden, D. Nayduch, J. G. Scott, E. R. Burgess, A. C. Gerry, P. E. Kaufman, J. Thomson, V Pickens, E. T. Machtinger

Summary: The house fly is a global pest that carries pathogens and causes significant economic losses. Effective management requires a combination of cultural, mechanical, biological control, and insecticide use. Research is needed to improve monitoring systems, develop faster-killing strains, and explore new attractants for traps. Understanding the fly microbiome and developing system-specific IPM strategies are also important for controlling house flies in different animal systems.

JOURNAL OF INTEGRATED PEST MANAGEMENT (2021)

Article Entomology

Stable Fly (Diptera: Muscidae)-Biology, Management, and Research Needs

K. Rochon, J. A. Hogsette, P. E. Kaufman, P. U. Olafson, S. L. Swiger, D. B. Taylor

Summary: Stable flies are global pests of livestock, companion animals, and humans, causing economic losses and transmitting diseases. The challenges in managing these flies lie in their developmental habitats, high mobility, and ability to exploit cultural practices. An integrated pest management approach incorporating cultural, mechanical, biological, and chemical control options is essential for effective stable fly management.

JOURNAL OF INTEGRATED PEST MANAGEMENT (2021)

Article Horticulture

Evaluation of the Microbiological Quality of Fresh Cilantro, Green Onions, and Hot Peppers from Different Types of Markets in Three US States

Yi Su, Wei-Yea Hsu, Tung-Shi Huang, Amarat Simonne

Summary: Consumer demand for a healthier lifestyle has led to an increase in the consumption of fresh produce and herbs as flavoring agents, with a preference for locally produced items. The study found that cilantro had the highest APC among tested produce, while national chains had lower APC levels compared to farmers' markets and locally owned grocery stores. No human pathogens were detected in any of the tested produce samples.

HORTICULTURAE (2021)

No Data Available