Multi-stage resistance to Zymoseptoria tritici revealed by GWAS in an Australian bread wheat diversity panel

Septoria tritici blotch (STB) has been ranked the third most important wheat disease in the world, threatening a large area of wheat production. Although major genes play an important role in the protection against Zymoseptoria tritici infection, the lifespan of their resistance unfortunately is very short in modern wheat production systems. Combinations of quantitative resistance with minor effects, therefore, are believed to have prolonged and more durable resistance to Z. tritici. In this study, new quantitative trait loci (QTLs) were identified that are responsible for seedling-stage resistance and adult-plant stage resistance (APR). More importantly was the characterisation of a previously unidentified QTL that can provide resistance during different stages of plant growth or multi-stage resistance (MSR). At the seedling stage, we discovered a new isolate-specific QTL, QSt.wai.1A.1. At the adult-plant stage, the new QTL QStb.wai.6A.2 provided stable and consistent APR in multiple sites and years, while the QTL QStb.wai.7A.2 was highlighted to have MSR. The stacking of multiple favourable MSR alleles was found to improve resistance to Z. tritici by up to 40%.

Automated summary

Septoria tritici blotch (STB) is recognized as the third most important wheat disease worldwide, affecting a significant portion of wheat production. This study identified new quantitative trait loci (QTLs) responsible for seedling-stage resistance and adult-plant stage resistance (APR) against Zymoseptoria tritici. Additionally, a previously unidentified QTL providing multi-stage resistance (MSR) was characterized. Stacking multiple favorable MSR alleles was found to significantly improve resistance to Z. tritici.