Article
Environmental Sciences
Jang Yeon Cho, Sol Lee Park, Hong-Ju Lee, Sang Hyun Kim, Min Ju Suh, Sion Ham, Shashi Kant Bhatia, Ranjit Gurav, See-Hyoung Park, Kyungmoon Park, Dongwon Yoo, Yung-Hun Yang
Summary: The study identified a thermotolerant and halotolerant bacteria, Bacillus sp. JY14, capable of degrading various PHBs, including other similar bioplastics, under different environmental conditions.
Article
Polymer Science
Sol Lee Park, Jang Yeon Cho, Su Hyun Kim, Shashi Kant Bhatia, Ranjit Gurav, See-Hyoung Park, Kyungmoon Park, Yung-Hun Yang
Summary: The study identified Microbulbifer sp. SOL66 as a highly efficient strain in degrading PHB, with the ability to completely degrade PHB within a short period under experimental conditions. Additionally, the addition of xylose and ammonium sulfate can enhance its degradation activity.
Article
Biotechnology & Applied Microbiology
Su Hyun Kim, Nara Shin, Suk Jin Oh, Jeong Hyeon Hwang, Hyun Jin Kim, Shashi Kant Bhatia, Jeonghee Yun, Jae-Seok Kim, Yung-Hun Yang
Summary: This study optimized the feasibility of the lyophilization method for microorganisms involved in bioplastic degradation and incorporated protective reagents to increase their viability and activity. Raffinose was selected as the most effective protective reagent, and bacterial activity was successfully maintained under different storage conditions. This method enhances the applicability of bioplastic-degrading bacterial strains and promotes the practical application of microorganisms in bioplastic degradation.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Sol Lee Park, Jang Yeon Cho, Su Hyun Kim, Hong-Ju Lee, Sang Hyun Kim, Min Ju Suh, Sion Ham, Shashi Kant Bhatia, Ranjit Gurav, See-Hyoung Park, Kyungmoon Park, Yun-Gon Kim, Yung-Hun Yang
Summary: This study identified a strain, Microbulbifer sp. SOL03, with poly-3-hydroxybutyrate (PHB) degradation activity and determined the optimal degradation conditions to be a temperature of 37℃, the addition of 3% NaCl, and no additional carbon sources. The strain achieved a PHB degradation yield of almost 97% after 10 days.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Fatimah S. Al-Khattaf, Mysoon M. Al-Ansari, Murali Kannan Maruthamuthu, L. Dyona, Paul Agastian
Summary: The study aimed to degrade poly-beta-hydroxybutyrate (P(3HB)) in the sequencing batch biofilm reactor (SBBR) using biocatalyst, with enrichment method successfully isolating P(3HB) degrading bacteria such as Pseudomonas aeruginosa KS10 and Nitrobacter vulgaris SW1. Results showed that these bacteria were effective in degrading P(3HB), reducing environmental pollution associated with petroleum-based polymers.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Mousumi Bhattacharyya, Rinita Dhar, Suman Basu, Avijit Das, Darren M. M. Reynolds, Tapan K. K. Dutta
Summary: This study investigates the catabolic potential of Mycolicibacterium sp. strain MBM in the degradation of DEHP. The strain is able to utilize both low- and high-molecular-weight phthalate diesters and can grow under moderately halotolerant conditions. This suggests that strain MBM may have potential use in the bioremediation of phthalate diesters.
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemistry & Molecular Biology
Maria Swiontek Brzezinka, Agnieszka Richert, Agnieszka Kalwasinska, Joanna Swiatczak, Edyta Deja-Sikora, Maciej Walczak, Marta Michalska-Sionkowska, Katarzyna Piekarska, Beata Kaczmarek-Szczepanska
Summary: The research aimed to isolate biofilm-forming bacteria capable of degrading PHB with PHMG derivatives. Different derivatives showed varying effects on biodegradation and biofilm formation in water and compost, with PHMG polyethylene wax granulate significantly inhibiting BOD at high concentrations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Yun-Han Fu, Zhi-Cheng Wu, Yan-Hui Kong, Xue-Wei Xu, Cong Sun, Yue-Hong Wu
Summary: Three strains, TT30(T), TT37(T) and L3(T), were isolated from tidal flat samples. These strains were Gram-stain-negative, non-motile and rod shaped. They were able to grow in medium containing varying concentrations of NaCl and at different pH and temperature ranges.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Rosa Turco, Gabriella Santagata, Iolanda Corrado, Cinzia Pezzella, Martino Di Serio
Summary: This review discusses the current research status and challenges of polyhydroxyalkanoates (PHA) as green alternative materials, focusing on the properties of polyhydroxybutyrate (PHB) and strategies for improving its performance and production process.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lionel F. Longe, Laurent Michely, Antoine Gallos, Agustin Rios De Anda, Henri Vahabi, Estelle Renard, Michel Latroche, Florent Allais, Valerie Langlois
Summary: This study used three novel additives based on ferulic acid esterified with different alcohols to improve the mechanical properties of poly(3-hydroxybutyrate) (PHB). The additive BDF significantly increased the processing window and elongation at break of PHB. The highly thermally stable additive also enhanced the fire-retardant property of the material and exhibited antioxidant properties.
BIOENGINEERING-BASEL
(2022)
Article
Multidisciplinary Sciences
Nguyen Thi Tam Thu, Le Huy Hoang, Pham Kien Cuong, Nguyen Viet-Linh, Tran Thi Huyen Nga, Dang Dinh Kim, Yoong Kit Leong, Le Thi Nhi-Cong
Summary: Scientists and the biodegradable polymer market are interested in polyhydroxyalkanoates (PHAs) due to their high biodegradability and processing flexibility. However, there is limited research on PHAs-producing marine yeast. In this study, a halophilic yeast strain isolated from Spratly Island in Vietnam was found to synthesize PHAs when cultured in Zobell marine agar medium containing Nile red dye. Under optimal growth conditions, the strain achieved a poly(3-hydroxybutyrate) content of 43.4% and a concentration of 1.8 gL(-1) after 7 days of cultivation. The produced poly(3-hydroxybutyrate) showed excellent biodegradability with a degradation rate of 28% after 28 days of incubation in sea water.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Zedong Jiang, Liufei Long, Meifang Liang, Hebin Li, Yanhong Chen, Mingjing Zheng, Hui Ni, Qingbiao Li, Yanbing Zhu
Summary: In this study, a new beta-glucosidase MaGlu1A was cloned from a marine bacterium, showing optimal activity at 40 degrees C and pH 4.5, substrate preference for aryl-beta-glycosidic bonds with glucose, fucose, and galactose moieties, and strong stimulation by supplemental glucose. The enzymatic characterization of MaGlu1A provides valuable information for its practical applications.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Zhipeng Li, Xiaoyi Huang, Yuxi Guo, Chenghao Zhang, Liang Yang, Xiping Du, Hui Ni, Xuchu Wang, Yanbing Zhu
Summary: In this study, a mass spectrometry-based quantitative analysis was used to study the degradation pathway of alginate polysaccharide in the bacterial strain Microbulbifer sp. ALW1. The results showed that ALW1 could effectively degrade alginate polysaccharide into disaccharides and trisaccharides within 12 hours. Proteome analysis identified numerous proteins involved in the digestion and metabolism of alginate polysaccharide. This study provides important insights into the genetic basis of alginate catabolism in ALW1 and may guide the utilization of this bacterial strain for efficient production of alginate oligosaccharides.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Polymer Science
Samiris Cocco Teixeira, Taila Veloso de Oliveira, Paulo Fortes-Da-Silva, Paulo A. A. Raymundo-Pereira, Alane Rafaela Costa Ribeiro, Lais Fernanda Batista, Nathalia O. O. Gomes, Paulo Cesar Stringheta, Nilda de Fatima Ferreira Soares
Summary: To evaluate the biodegradability of cellulose acetate (CA) films plasticized with different concentrations of glycerol (GLY) or triethyl citrate (TEC), scanning electron microscopy (SEM), Fourier transfer infrared spectroscopy (FTIR), and thermogravimetric analyses (TGA) were conducted over a period of 180 days. Plasticizer exudation was observed through changes in visual appearance and mass loss, with the percentages ranging from 39.141% to 0.524% for TEC and from 20.663% to 0.488% for GLY plasticizer based films. Infrared and TGA analyses also showed changes in polymer degradation peaks, but there was no evidence of film biodegradation during the experimental period. This systematic approach can be applied to study other polymers and biodegradation conditions.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Qian Zhang, Yuanyuan Gui, Luying Zhao, Ao Zhang, Liping Fu, Zhe Cao, Jiang Li
Summary: In this study, the iota-carrageenase gene Car1293 was obtained from Microbulbifer sp. YNDZ01, and its sequence, protein structure, enzymatic properties, enzymatic digestion products, and anti-inflammatory activity were investigated. The results showed that Car1293 encodes a novel enzyme that can hydrolyze carrageenan into CGOS-DP8 with significant anti-inflammatory activity. This study fills a gap in the research on the biological activity of oligosaccharides in iota-carrageenan and provides promising data for the development of natural anti-inflammatory agents.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Chemistry, Physical
Chayanika Putatunda, Manya Behl, Preeti Solanki, Samriti Sharma, Shashi Kant Bhatia, Abhishek Walia, Ravi Kant Bhatia
Summary: Biohydrogen is considered a versatile future fuel that can replace fossil fuels in various sectors, and photofermentation is an attractive approach due to its efficiency and reduced environmental impacts. However, there are still significant challenges in commercial biohydrogen production through photofermentation. This review provides updated information on new technologies and discusses the prospects for the future of green biohydrogen production.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Polymer Science
Beom-Jung Kang, Jong-Min Jeon, Shashi Kant Bhatia, Do-Hyung Kim, Yung-Hun Yang, Sangwon Jung, Jeong-Jun Yoon
Summary: Coffee waste can be used to extract oil and produce polyhydroxyalkanoate (PHA). The extracted oil can also be used for biohydrogen production. The oil extraction yield was 14.4% and accounted for 97% of the oil in the waste. The extracted waste had a higher sugar concentration and resulted in more hydrogen production. Additionally, using the extracted oil for culture resulted in higher cell dry weight, PHA production, and PHA content.
Article
Chemistry, Physical
J. Rajesh Banu, S. Kavitha, R. Yukesh Kannah, J. Jayakodi, Gopalakrishnan Kumar, Vinay Kumar Tyagi, Shashi Kant Bhatia
Summary: The study investigates the possibility of enhancing biomass solubilization and economic output through a combinatorial pretreatment, which combines the use of a surfactant (sodium dodecyl sulfate) with a mechanical pretreatment induced by a disperser. The results show that this combinatorial pretreatment reduces energy input and improves solubilization and methane production, leading to higher net profit compared to the conventional disperser-mediated disintegration process.
SUSTAINABLE ENERGY & FUELS
(2023)
Article
Biochemistry & Molecular Biology
Byungchan Kim, Suk Jin Oh, Jeong Hyeon Hwang, Hyun Jin Kim, Nara Shin, Shashi Kant Bhatia, Jong-Min Jeon, Jeong-Jun Yoon, Jaehung Yoo, Jungoh Ahn, Jung -Ho Park, Yung-Hun Yang
Summary: Petrochemical-based plastics are environmentally detrimental and difficult to degrade, making them a major cause of pollution. As an alternative, pol-yhydroxybutyrate (PHB) has similar properties, but its high production cost poses a significant challenge to industrialization. This study utilized crude glycerol as a carbon source to improve the efficiency of PHB production, selecting Halomonas taeanenisis YLGW01 as the strain of choice due to its salt tolerance and high glycerol consumption rate. Through medium optimization and activated carbon treatment, PHB production was maximized, resulting in 10.5 g/L of PHB with 60% PHB content in fed-batch fermentation. The physical properties of the produced PHB were analyzed, including weight average molecular weight, number average molecular weight, and polydispersity index. The extracted intracellular PHB demonstrated improved flexibility and decreased brittleness compared to authentic film. This study confirms the potential of YLGW01 for industrial PHB production using crude glycerol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemical Research Methods
Young Su Kim, Hye-Jeong Lee, Gabriella Aphrodita Handoko, Jaehui Kim, Minho Won, Jung-Ho Park, Jungoh Ahn
Summary: In this study, high-level production of bioactive recombinant human keratinocyte growth factor 2 (KGF-2) was achieved for large-scale production. 6HFh8-KGF-2 was purified using nickel affinity chromatography. The results showed that the high-level expression of 6HFh8-KGF-2 in Escherichia coli could effectively induce MCF-7 cell proliferation.
PROTEIN EXPRESSION AND PURIFICATION
(2023)
Editorial Material
Polymer Science
Shashi Kant Bhatia
Review
Biotechnology & Applied Microbiology
Ankesh Ahirwar, Swati Das, Sovik Das, Yung-Hun Yang, Shashi Kant Bhatia, Vandana Vinayak, Makarand Madhao Ghangrekar
Summary: Photosynthetic microbial fuel cells (PMFCs) are environmentally friendly fuel cells that use light to produce renewable bioelectricity. Algae assisted PMFCs have advantages such as rapid growth, smaller space requirement, production of free oxygen, and value-added products. This review highlights the application and advantages of algae assisted PMFCs for bioelectricity generation and recovery of value-added products. The low cost and potential for resource recovery using PMFCs are also emphasized.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Editorial Material
Microbiology
Debarati Paul, Justyna Bohacz, Shashi Kant Bhatia
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Green & Sustainable Science & Technology
Vishal Ahuja, Arvind Kumar Bhatt, Balasubramani Ravindran, Yung-Hun Yang, Shashi Kant Bhatia
Summary: Biomass gasification produces syngas containing CO, H2, H2S, CO2, N2, N, and tar compounds. The carbon in syngas can be utilized for the production of valuable chemicals, but chemical sequestration requires high temperature and pressure with heavy metal catalysts. Microorganisms like algae and bacteria have the potential for carbon sequestration. However, scale-up and commercialization face challenges such as mass transfer inefficiency, microbial contamination, inconsistent syngas composition, and the need for a clean-up process. This review summarizes recent advances and challenges in syngas production and utilization, with a focus on alcohol and energy-related products.
Article
Agricultural Engineering
Tirath Raj, Sandhya Sompura, K. Chandrasekhar, Sushil Kumar Singh, Srinath Pandey, Lalit Kumar Singh, Manish Singh Rajput, Deepak Kumar, Shashi Kant Bhatia, Anil Kumar Patel, Reeta Rani Singhania
Summary: This paper examines the issues in municipal solid waste production and management, and discusses the generation of diverse bioproducts from organic waste. By converting organic waste into fuel and chemicals, it is possible to reduce waste, increase energy output, and provide additional healthcare commodities. This contributes to achieving high levels of energy security, environmental protection, and a more robust bioeconomy.
BIOMASS & BIOENERGY
(2023)
Review
Environmental Sciences
R. Anu Alias Meena, J. Merrylin, J. Rajesh Banu, Shashi Kant Bhatia, Vinod Kumar, Grzegorz Piechota, Gopalakrishnan Kumar
Summary: The voluminous POME is a threat to the environment, but it can be converted into biorefinery products. The review discusses the risk assessment of POME and proposes using fish species, including invasive ones, for toxicants identification. Various treatments and technologies have been investigated, such as adsorption, anaerobic digestion, and microalgae cultivation, with emphasis on advanced bioreactors. The production of biodiesel from POME shows promise, despite economic challenges.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biotechnology & Applied Microbiology
Sang Hyun Kim, Jeong Hyeon Hwang, Hyun Joong Kim, Suk Jin Oh, Hyun Jin Kim, Nara Shin, Sang-Hyoun Kim, Jeong-Hoon Park, Shashi Kant Bhatia, Yung-Hun Yang
Summary: The study developed Clostridium acetobutylicum as a superior biohydrogen producer by introducing glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to enhance hydrogen production. The strain CAC824-G containing gapC showed a 66.3% higher hydrogen production than the wild-type strain, with increased NADH and NADPH pools. Overexpression of gapC resulted in increased hydrogen production even in the presence of inhibitors.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Sion Ham, Hyun Jin Kim, Nara Shin, Jeong Hyeon Hwang, Suk Jin Oh, Jun Young Park, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: This study demonstrates the efficient and economical production of GABA by immobilization and continuous production in a small-scale reactor. By optimizing the conditions, over 95% conversion of 600 mM monosodium glutamate to GABA can be achieved within 3 hours, and the immobilized cells can be reused for up to 15 times. Furthermore, a continuous production system operating in a 14 mL scale reactor produced 165 g of GABA after 96 hours of continuous operation, after optimizing the buffer concentration, substrate concentration, and flow rate.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Agricultural Engineering
Neha Chandel, Kinnri Jain, Ankit Jain, Tirath Raj, Anil Kumar Patel, Yung-Hun Yang, Shashi Kant Bhatia
Summary: Cellulose is a versatile natural biopolymer that can be obtained from plants or produced through microbial fermentation. It possesses desirable properties, including biodegradability, biocompatibility, noncytotoxicity, nongenotoxicity, and easy modification, making it highly suitable for biomedical and pharmaceutical applications. Surface modification, blending with other natural polymers, or incorporation of metal-based nanoparticles can enhance its thermal, surface, and mechanical properties. Cellulose-based materials, such as nanocomposites, graft copolymers, and nanocrystals, have been extensively investigated for use in various industries, including food, textiles, wastewater treatment, biomedical engineering, and drug delivery systems. This review provides insights into cellulose production and modification for the development of high-value-added materials in biomedical applications.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Editorial Material
Green & Sustainable Science & Technology
Shashi Kant Bhatia