Article
Biotechnology & Applied Microbiology
Susanna Gevorgyan, Robin Schubert, Mkrtich Yeranosyan, Lilit Gabrielyan, Armen Trchounian, Kristina Lorenzen, Karen Trchounian
Summary: The research explores the potential of raw royal jelly in green synthesis of silver nanoparticles and its antibacterial activity, with a higher sensitivity observed on Gram-negative bacteria.
Article
Biotechnology & Applied Microbiology
Tabbisa Namulinda, Lei-Lei Bao, Justus Kwetegyeka, Ivan Gumula, Yi-Jia Yan, Zhi-Long Chen
Summary: This study synthesized silver nanoparticles (PG-Ag NPs) using Photinia glabra fruit extract and evaluated their antibacterial and anticancer activities. The results showed that PG-Ag NPs exhibited strong antibacterial activity against Escherichia coli and Staphylococcus aureus, and significant cytotoxicity towards esophageal cancer cells. These NPs have great potential as a biomaterial for various biomedical applications.
Article
Multidisciplinary Sciences
Aliya Yousaf, Muhammad Waseem, Aneela Javed, Sofia Baig, Bushra Ismail, Ayesha Baig, Irum Shahzadi, Shamyla Nawazish, Iftikhar Zaman
Summary: This study synthesized silver nanoparticles using Taxus wallichiana leaf extract and evaluated their antibacterial and anticancer properties. The nanoparticles showed significant antibacterial activity against Salmonella typhi and Staphylococcus aureus, as well as cytotoxic activity against U251 brain cancer cells.
Article
Chemistry, Physical
Yage Xing, Xingmei Liao, Xiaocui Liu, Wenxiu Li, Ruihan Huang, Jing Tang, Qinglian Xu, Xuanlin Li, Jinze Yu
Summary: The study synthesized silver nanoparticles (AgNPs) from biological waste and found that AgNPs exhibited antibacterial properties against Escherichia coli and Staphylococcus aureus when synthesized using different concentrations of mango peel extract, particularly showing significant effects at 0.20 g/mL concentration.
Article
Microbiology
Siti Nur Amalina Mohamad Sukri, Kamyar Shameli, Sin-Yeang Teow, Jactty Chew, Li-Ting Ooi, Michiele Lee-Kiun Soon, Nur Afini Ismail, Hassan Moeini
Summary: This study presents a green synthesis approach for the fabrication of zinc oxide-silver nanoparticles (ZnO-Ag-NPs) using Punica granatum fruit peels extract as a natural reducing and stabilizing agent. The synthesized ZnO-Ag-NPs showed potent antibacterial activity against a broad spectrum of bacterial strains, particularly Gram-positive bacteria, with superior inhibition activity compared to ZnO-NPs. Moreover, ZnO-Ag-NPs showed a dose-dependent anti-proliferative effect on colorectal-, lung-, and cervical cancer cells.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Sohail Simon, Nicole Remaliah Samantha Sibuyi, Adewale Oluwaseun Fadaka, Samantha Meyer, Jamie Josephs, Martin Opiyo Onani, Mervin Meyer, Abram Madimabe Madiehe
Summary: Silver nanoparticles with outstanding antimicrobial activities have attracted interest in biomedical applications. Using plant extracts to synthesize AgNPs provides a safe, cost-effective, and environmentally friendly approach, producing biocompatible AgNPs with enhanced properties for diverse applications.
Article
Chemistry, Inorganic & Nuclear
Ruby, Aryan, Mohan Singh Mehata
Summary: An environmentally friendly synthesis process was used to synthesize silver nanoparticles (AgNPs) using a remedial plant. The AgNPs showed high stability and demonstrated potential applications in antibacterial activity, dye degradation, and hydrogen peroxide detection.
DALTON TRANSACTIONS
(2022)
Article
Biotechnology & Applied Microbiology
Irum Shahzadi, Syed Munawar Aziz Shah, Mohammad Maroof Shah, Tariq Ismail, Nighat Fatima, Maria Siddique, Ummara Waheed, Ayesha Baig, Aisha Ayaz
Summary: The study examines the antioxidant, anticancer, antibacterial, and antifungal potential of biosynthesized silver nanoparticles (TpAgNPs) using plant extract. The findings reveal the critical role of phenolics, flavonoids, and anthocyanins in the production of TpAgNPs. TpAgNP6 exhibits low cytotoxicity and high antibacterial and antifungal activity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yujia Liang, Bin Tang, Aarushi Sharma, Dinidu Perera, Benjamin James Allardyce, Sourabh Ghosh, Hannes C. Schniepp, Rangam Rajkhowa
Summary: Silver nanoparticles were successfully synthesized on microfibrillated silk exfoliated from domesticated silkworm silk fibers for the first time, resulting in robust and flexible protein papers with excellent handling properties. The AgNPs coated MFS showed strong wash fastness and mechanical properties comparable to MFS papers without nanoparticles, while exhibiting improved tensile properties and antibacterial activity. This study lays the foundation for the development of smart protein papers based on silk fiber and functional nanomaterials.
MACROMOLECULAR BIOSCIENCE
(2021)
Review
Pharmacology & Pharmacy
Omnia Ahmed, Nicole Remaliah Samantha Sibuyi, Adewale Oluwaseun Fadaka, Madimabe Abram Madiehe, Ernest Maboza, Mervin Meyer, Greta Geerts
Summary: Oral diseases are common and can lead to serious health problems. This study explores the use of nanotechnology to improve traditional preventive measures, focusing on silver nanoparticles (AgNPs) for dental therapy. Biogenic AgNPs synthesized from natural products are considered more biocompatible and environmentally friendly.
Article
Chemistry, Physical
Mohammad Ali Ebrahimzadeh, Zahra Hashemi, Mahsa Mohammadyan, Mahdi Fakhar, Sobhan Mortazavi-Derazkola
Summary: A simple and low-cost method was developed for the synthesis of silver nanoparticles using Scrophularia striata leaf extract. The synthesized nanoparticles showed excellent antibacterial, anticancer, and antiparasitic activities, making them a promising candidate for various biomedical applications.
SURFACES AND INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Omaish Alqahtani, Kiran K. Mirajkar, K. R. Anil Kumar, Mater H. Mahnashi, Ibrahim Ahmed Shaikh, Susweta Mitra, Harshitha Nagaraj, Sunil S. More, Uday M. Muddapur, Aejaz Abdullatif Khan, P. Renuka Sudarshan
Summary: This study evaluated the effect of green synthesized silver nanoparticles on the biochemical constituents of rice plants infected with blast. The results showed that the application of nanoparticles increased the activity of antioxidative enzymes and biochemical constituents in rice plants, especially at concentrations of 30ppm and 50ppm, which exhibited the highest resistance towards the pathogen.
Article
Polymer Science
Ngoc-Thang Nguyen, Tien-Hieu Vu, Van-Huan Bui
Summary: To address the transmission of pathogenic microorganisms in leather shoes, researchers modified pig leather with bio-synthesized silver nanoparticles from Piper betle L. leaf extract. The modified leather exhibited excellent antibacterial activity and met the requirements for making hygienic shoes.
Article
Infectious Diseases
Yahya S. Alqahtani, Amal Bahafi, Kiran K. Mirajkar, Rakshith Rudrapura Basavaraju, Susweta Mitra, S. Shailaja, Sunil S. More, Uday M. Muddapur, Aejaz Abdullatif Khan, P. Renuka Sudarshan, Ibrahim Ahmed Shaikh
Summary: Around 35% of the world's population relies on wheat as their main crop, with China and India being the biggest consumers. In this study, green synthesized silver nanoparticles were found to enhance the antioxidative enzymes and biochemical constituents in wheat crops infected with spot blotch disease. Additionally, these nanoparticles exhibited antibacterial activity against multidrug-resistant bacteria.
Article
Engineering, Environmental
Veronica Rocha, Pedro Ferreira-Santos, Zlatina Genisheva, Eduardo Coelho, Isabel C. Neves, Teresa Tavares
Summary: Stable silver nanoparticles with spherical morphology and variable size were synthesized using aqueous Eucalyptus globulus leaves extract. These nanoparticles exhibit dual-functional properties of antibacterial activity and dye photodegradation.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Biophysics
Liana Vanyan, Karen Trchounian
Summary: This study investigates the proton/potassium exchange mechanism of Escherichia coli under different glucose concentrations and finds that the HyfBDF subunits play a crucial role in sensing glucose concentration and regulating proton motive force generation.
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
(2022)
Article
Multidisciplinary Sciences
Arayik Martirosyan, Sven Falke, Deborah McCombs, Martin Cox, Christopher D. Radka, Jan Knop, Christian Betzel, Lawrence J. DeLucas
Summary: Microgravity conditions have been found to improve protein crystallization by reducing impurity incorporation and increasing crystal quality. Experiments conducted on the International Space Station provide valuable insights into macromolecular transport phenomena.
Article
Biochemistry & Molecular Biology
Janine Hochmair, Christian Exner, Maximilian Franck, Alvaro Dominguez-Baquero, Lisa Diez, Hevila Brognaro, Matthew L. Kraushar, Thorsten Mielke, Helena Radbruch, Senthil Kaniyappan, Sven Falke, Eckhard Mandelkow, Christian Betzel, Susanne Wegmann
Summary: This study reveals that molecular crowding and coacervation with RNA synergize to induce Tau condensation in the cytosol, enhancing microtubule bundling and spatial arrangement. Different Tau condensates efficiently induce pathological Tau aggregates, including accumulations at the nuclear envelope. These findings provide insights into the regulation of Tau condensate formation and function.
Article
Multidisciplinary Sciences
Aline Melro Murad, Hevila Brognaro, Sven Falke, Jasmin Lindner, Markus Perbandt, Celestin Mudogo, Robin Schubert, Carsten Wrenger, Christian Betzel
Summary: The study solved and refined the crystal structure of the key enzyme SaDHNA in the vitamin K-2 pathway of Staphylococcus aureus, revealing a functional tetramer and conducting complementary activity assays and site-directed mutagenesis studies. These findings will support future drug discovery and design investigations to inhibit the bacterial vitamin K-2 synthesis pathway.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Amr Moustafa, Markus Perbandt, Eva Liebau, Christian Betzel, Sven Falke
Summary: In this study, an extracellular Cu/Zn-SOD from O. volvulus (OvEC-SOD) was cloned, purified and crystallized to gain insight into a potential drug target for combating onchocerciasis.
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Wiebke Ewert, Sebastian Guenther, Francesca Miglioli, Sven Falke, Patrick Y. A. Reinke, Stephan Niebling, Christian Guenther, Huijong Han, Vasundara Srinivasan, Hevila Brognaro, Julia Lieske, Kristina Lorenzen, Maria M. Garcia-Alai, Christian Betzel, Mauro Carcelli, Winfried Hinrichs, Dominga Rogolino, Alke Meents
Summary: Researchers studied the papain-like protease (PLpro) of SARS-CoV-2 and identified six compounds that bind to PLpro. These compounds interact with the substrate binding sites of PLpro and have the potential to be lead structures for antiviral drugs.
FRONTIERS IN CHEMISTRY
(2022)
Article
Energy & Fuels
Kairat Bekbayev, Satenik Mirzoyan, Akerke Toleugazykyzy, Dinara Tlevlessova, Anait Vassilian, Anna Poladyan, Karen Trchounian
Summary: Escherichia coli can anaerobically utilize various carbon sources for hydrogen production. Mixed carbon sources can enhance H-2 production compared to single waste. The H-2 production and growth rate differ between wild type cells and multiple mutants in mixed waste conditions.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Chemistry, Physical
Mengying Wang, Roland Thuenauer, Robin Schubert, Susanna Gevorgyan, Kristina Lorenzen, Hevila Brognaro, Christian Betzel
Summary: This study investigates the assembling kinetics, structures, and physicochemical properties of Alpha-Synuclein (ASN) assemblies under the influence of ionic strength and a pulsed electric field. The results demonstrate that ASN monomers can form liquid dense clusters via liquid-liquid phase separation (LLPS) and mesoscopic assemblies with ordered internal structures and high thermostabilities at a specific ionic strength of 50 mM NaCl. Furthermore, pulsed electric fields and ionic strength can effectively modulate the thermostability and autofluorescence of these mesoscopic ASN assemblies.
Article
Multidisciplinary Sciences
Prabhu Rajaiah Prince, Janine Hochmair, Hevila Brognaro, Susanna Gevorgyan, Maximilian Franck, Robin Schubert, Kristina Lorenzen, Selin Yazici, Eckhard Mandelkow, Susanne Wegmann, Christian Betzel
Summary: Tau is a neuronal protein in the central nervous system that can form neurofibrillary tangles in Alzheimer's disease. In vitro experiments show that aggregated Tau can be induced by polyanionic co-factors, such as RNA or heparin. These co-factors can also trigger Tau condensates, which over time develop pathological aggregation potential. However, electrostatic interactions with the drug suramin can induce Tau condensation without pathological aggregation, reducing the potential for cellular Tau aggregation. This discovery suggests a new therapeutic approach for aberrant Tau phase separation using small anionic compounds.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Meri Iskandaryan, Syuzanna Blbulyan, Mayramik Sahakyan, Anait Vassilian, Karen Trchounian, Anna Poladyan
Summary: The addition of L-amino acids and vitamins from yeast extract enhances the synthesis and activity of O-2-tolerant Hyds enzymes in Ralstonia eutropha H16. The presence of L-amino acids leads to a decrease in pH and reduction in ORP, promoting bacterial growth and Hyds enzyme activity. These findings are crucial for optimizing the production of O-2-tolerant Hyds enzymes as anode biocatalysts.
Article
Biophysics
Heghine Gevorgyan, Tamara Abaghyan, Margarita Mirumyan, Konstantin Yenkoyan, Karen Trchounian
Summary: Short-chain fatty acids like PPA and VP can disrupt the growth, viability, bioenergetic, and biochemical properties of Escherichia coli and Enterococcus hirae bacteria by altering their FOF1-ATPase activity and H+ flux. PPA affects membrane permeability in E. hirae, while VP has a selective effect on bacteria's specific growth rate. These findings highlight the importance of gut microbiota in the development of autism spectrum disorders.
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
(2023)
Article
Biology
Patrick Y. A. Reinke, Edmarcia Elisa de Souza, Sebastian Guenther, Sven Falke, Julia Lieske, Wiebke Ewert, Jure Loboda, Alexander Herrmann, Aida Rahmani Mashhour, Katarina Karnicar, Aleksandra Usenik, Natasa Lindic, Andreja Sekirnik, Viviane Fongaro Botosso, Glaucia Maria Machado Santelli, Josana Kapronezai, Marcelo Valdemir de Araujo, Taiana Taina Silva-Pereira, Antonio Francisco de Souza Filho, Mariana Silva Tavares, Lizdany Florez-Alvarez, Danielle Bruna Leal de Oliveira, Edison Luiz Durigon, Paula Roberta Giaretta, Marcos Bryan Heinemann, Maurice Hauser, Brandon Seychell, Hendrik Boehler, Wioletta Rut, Marcin Drag, Tobias Beck, Russell Cox, Henry N. Chapman, Christian Betzel, Wolfgang Brehm, Winfried Hinrichs, Gregor Ebert, Sharissa L. Latham, Ana Marcia de Sa Guimaraes, Dusan Turk, Carsten Wrenger, Alke Meents
Summary: The drug candidate Calpeptin has been identified as effective against SARS-CoV-2 by targeting the cysteine cathepsin enzyme, reducing viral load. Targeting host proteins provides an advantage due to their mutational stability compared to highly mutable viral targets.
COMMUNICATIONS BIOLOGY
(2023)
Article
Microbiology
Anush Babayan, Anait Vassilian, Karen Trchounian
Summary: Escherichia coli can produce molecular hydrogen (H-2) through mixed-acid fermentation, and this production can be influenced by the osmotic stress and pH conditions. Hyperosmotic stress and the addition of formate tended to stimulate H-2 production, while hypostress conditions and the addition of glycerol increased H-2 production in hybC and hyfG mutants. pH levels also played a role in modulating H-2 production, with hyperosmotic stress stimulating production at pH 6.5 and inhibiting production at pH 5.5 in different strains.
Article
Energy & Fuels
Satenik Mirzoyan, Hayarpi Aghekyan, Liana Vanyan, Anait Vassilian, Karen Trchounian
Summary: Coffee consumption generates a significant amount of waste, including coffee silverskin and spent coffee grounds. This study explores the use of coffee silverskin as a carbon source for bacterial growth and hydrogen production, demonstrating its potential as a cost-effective biomass and bio-H2 production technology.
INTERNATIONAL JOURNAL OF ENERGY RESEARCH
(2022)
