Article
Cell Biology
Sara Miguel-Jimenez, Blanca Pina-Beltran, Silvia Gimeno-Martos, Melissa Carvajal-Serna, Adriana Casao, Rosaura Perez-Pe
Summary: This study found NOX5 in ram spermatozoa and demonstrated that melatonin can prevent changes related to sperm capacitation by modulating NOX5 activity. This suggests that melatonin plays a role in antioxidant defense in ram sperm by influencing NOX5 during capacitation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Christelle Damon-Soubeyrand, Antonino Bongiovanni, Areski Chorfa, Chantal Goubely, Nelly Pirot, Luc Pardanaud, Laurence Piboin-Fragner, Caroline Vachias, Stephanie Bravard, Rachel Guiton, Jean-Leon Thomas, Fabrice Saez, Ayhan Kocer, Meryem Tardivel, Joel R. Drevet, Joelle Henry-Berger
Summary: The epididymis plays a crucial role in male fertility and has both secretory and immune functions. However, the organization of blood and lymphatic networks in the epididymis is still largely unknown.
Article
Reproductive Biology
Sara Miguel-Jimenez, Sonia Borao, Virginia Portoles-Bayod, Adriana Casao, Rosaura Perez-Pe
Summary: The study investigated the effects of season and sperm capacitation state on the chemotactic response of ram spermatozoa to melatonin. The results showed that melatonin can enhance sperm recovery rate and capacitation, indicating a potential chemotactic effect of melatonin on ram spermatozoa.
Article
Reproductive Biology
Saulo Menegatti Zoca, Emmalee J. Northrop-Albrecht, Julie A. Walker, Robert A. Cushman, George A. Perry
Summary: Sperm stored in the epididymis have a longer lifespan, but their motility increases and lifespan decreases upon ejaculation. This study aimed to identify protein differences between epididymis and ejaculated samples that are associated with longevity. The analysis revealed that energy metabolism in the epididymis is more glycolytic and epididymal sperm have more antioxidants, which may contribute to maintaining sperm in a quiescent state.
Article
Biochemistry & Molecular Biology
Christopher Casciaro, Hirotaka Hamada, Alisa Kostaki, Stephen G. Matthews
Summary: Approximately 1%-3% of the adult population are treated with sGCs, and previous studies have shown that pre-conception adversities in males may affect offspring's neuroendocrine function and behavior through miRNA changes in sperm. This study aimed to investigate the region-specific effects of sGC exposure on sperm miRNA profile in the epididymis. Results showed that Dex-exposure led to decreased expression of one miRNA in the caput and 31 miRNAs in the cauda. This indicates that sGCs target the epididymis to modify sperm miRNA but do not affect miRNA content during spermiation in the testes.
Review
Agriculture, Dairy & Animal Science
Hiba Ali Hassan, Guillaume Domain, Gaia Cecilia Luvoni, Rana Chaaya, Ann Van Soom, Eline Wydooghe
Summary: Epididymal semen from dogs and cats provides an additional source of gametes for preserving valuable genetics, especially in cases where ejaculation is not possible, castration is necessary, or unexpected death occurs. Understanding the differences between epididymal semen and ejaculated semen is crucial for optimizing cryopreservation and enhancing the applications of assisted reproductive technologies. Factors affecting epididymal semen quality and freezability need to be identified and addressed to improve post-thaw semen quality and ART outcomes.
Article
Agriculture, Dairy & Animal Science
Anna Wysokinska, Ewa Wojcik, Angelika Chlopik
Summary: Sperm morphometry is an important indicator of the quality of spermatozoa. A study on dog epididymides found that sperm collected from different segments varied in size, with the cauda having the smallest dimensions. Staining methods affected sperm dimensions, with different fixatives and reagents leading to variations in head size.
Review
Biochemistry & Molecular Biology
Francisca Felix, Catarina C. V. Oliveira, Elsa Cabrita
Summary: The study focuses on the effects of novel antioxidants in protecting fish cells, highlighting the importance of antioxidants in fish reproduction and their potential in improving gamete quality. The review discusses various types of antioxidants and their mechanisms of action, with a particular emphasis on the protective role of melatonin in preventing oxidative stress damage on fish spermatozoa.
Article
Veterinary Sciences
Athina Basioura, Georgia D. Brellou, Ioannis Tsakmakidis, Ioannis Vlemmas, Constantin M. Boscos, Nektarios Giadinis
Summary: Varicocele is a common pathological condition related to male fertility problems. This case report indicates that varicocele is associated with testis dysfunction and degradation of ram semen quality, mainly affecting motility and kinematics.
VETERINARY SCIENCES
(2022)
Article
Reproductive Biology
Baoyu Jia, Sameeullah Memon, Jiachong Liang, Chunrong Lv, Qionghua Hong, Guoquan Wu, Guobo Quan
Summary: Trehalose has been shown to enhance post-thaw quality of ram spermatozoa by modifying their protein profile, potentially through antioxidation, involvement in glycolysis, and increasing spermatozoa tolerance to various stresses.
Article
Reproductive Biology
Chuang Li, Hyun Ju Oh, Haixing Liu, Min Kyu Kim
Summary: Oxidative stress caused by low temperatures during in vitro preservation decreases the quality of boar spermatozoa. Schisandrin B (Sch-B) has been found to counteract oxidative stress in cells. This study aimed to investigate whether treatment with Sch-B could enhance the quality of boar sperm stored at 17 degrees C. Semen samples were divided into four groups and treated with different concentrations of Sch-B. The results showed that treatment with 0.1 mg/L Sch-B improved sperm motility, membrane integrity, and acrosome integrity compared to the control group. It also reduced lipid peroxidation and increased the fertilization ability of the sperm. The findings suggest that Sch-B treatment protects boar sperm from oxidative stress and improves their fertilization ability during liquid preservation.
Review
Multidisciplinary Sciences
Mohsen Ghaleb Al-Mutary
Summary: Despite the widespread use of frozen or refrigerated mammalian spermatozoa, their quality remains low due to sensitivity to injuries during preservation and thawing. Antioxidants have been employed in livestock spermatozoa preservation to maintain sperm quality and counteract oxidative stress.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Article
Reproductive Biology
Naisheng Lu, Xueyuan Jiang, Cong Zhang, Bushe Li, Weilong Tu, Hulong Lei, Wen Yao, Dong Xia
Summary: Researches have shown that melatonin (MLT) can improve oxidative damage and increase sperm motility for conservation sperms, but the mechanism is still unclear. In this study, the energy metabolism of sperm during preservation at 17 degrees C and activation at 37 degrees C was investigated. The results revealed that 1.0 μM MLT treatment affected the energy metabolism of sperm, decreasing ATP content and the mRNA abundance of mitochondrial DNA encoding genes at 17 degrees C, but increasing ATP content and the mRNA expressions of specific genes at 37 degrees C. This study suggests that MLT might regulate sperm ATP generation and antioxidative enzyme activity via MT1 in a temperature-dependent manner.
Article
Biochemistry & Molecular Biology
Tricia Rowlison, Pierre Comizzoli
Summary: Key protein LGALS3BP is transferred via extracellular vesicles between the epididymis and maturing sperm cells, and this transfer affects sperm fertilizing ability and developmental potential. Inhibition of LGALS3BP leads to decreased fertilization success and slower first cell cycles, demonstrating the importance of LGALS3BP in sperm function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Abouzar Najafi, Hossein Mohammadi, Seyed Davood Sharifi
Summary: The effect of different rutin concentrations on rams epididymal sperm was examined in this study. Cryopreservation of ram sperm in tris extender containing rutin showed improved motility, velocity, membrane integrity, and mitochondrial activity at 0.75 and 1 mM rutin concentrations. ATP, total antioxidant capacity, and glutathione peroxidase levels were significantly higher in the rutin 0.75 and 1 mM groups. Rutin at 0.75 and 1 mM also resulted in a lower concentration of reactive oxygen species and reduced apoptosis and dead sperm percentage.
SCIENTIFIC REPORTS
(2023)