Article
Multidisciplinary Sciences
Raviv Dharan, Yuwei Huang, Sudheer Kumar Cheppali, Shahar Goren, Petr Shendrik, Weisi Wang, Jiamei Qiao, Michael M. Kozlov, Li Yu, Raya Sorkin
Summary: The formation of migrasomes is a two-stage process. In the first stage, local swellings devoid of tetraspanin 4 form on retraction fibers due to membrane tension. In the second stage, tetraspanin 4 migrates to and binds onto these swellings, which grow and transform into migrasomes. Tetraspanin 4 recruitment is essential for migrasome growth and stabilization.
NATURE COMMUNICATIONS
(2023)
News Item
Optics
Xiaohe Zhang, Bing Gu, Cheng-Wei Qiu
Summary: Researchers have proposed a new method that can measure the weak force of a single microscopic particle.
LIGHT-SCIENCE & APPLICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Petr Shendrik, Gonen Golani, Raviv Dharan, Ulrich S. Schwarz, Raya Sorkin
Summary: Fusion of biological membranes plays a fundamental role in various physiological events. The energy barriers of fusion stages are tightly dependent on the mechanical and physical properties of the system, including membrane tension. This study reveals that membrane tension inhibits lipid mixing and increases the energy barrier of hemifusion stalk formation, leading to a delay in lipid mixing time.
Article
Multidisciplinary Sciences
Guillermo J. Amador, Dennis van Dijk, Roland Kieffer, Marie-Eve Aubin-Tam, Daniel Tam
Summary: The study introduced an active microrheology technique to investigate the physical properties of lipid membranes, revealing the significant impact of lipid structure on viscosity and fluid transmission properties. Ordered-phase and liquid disordered-phase lipid bilayers exhibit distinct differences in viscosity and intermonolayer friction.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Jie Yang, Huaizhou Jin, Yihao Liu, Yaya Guo, Yongli Zhang
Summary: Munc18 chaperones play a key role in mediating membrane fusion between vesicles and plasma membranes. Recent studies have revealed that Munc18-1 and Munc18-3 form ternary complexes with their cognate SNAREs, which facilitate the binding of SNAP-25 or SNAP-23 to conclude SNARE assembly. This study provides further insights into the structure, dynamics, and function of these template complexes. Our findings demonstrate that a highly dynamic template complex mediates efficient and specific SNARE assembly.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Physical
Hongru Ding, Pavana Siddhartha Kollipara, Linhan Lin, Yuebing Zheng
Summary: Optothermal tweezers, with superior manipulation capability at low optical intensity, are limited by implicit understanding of the working mechanism. By revealing the atomistic view of opto-thermo-electro-mechanic coupling, we have developed high-performance tweezers using low-concentrated electrolytes and demonstrated their efficiency in separating colloidal particles based on surface charge differences.
Article
Cell Biology
Cuifang Zhang, Tianqi Li, Shuyao Yin, Mingyi Gao, Helen He, Ying Li, Dong Jiang, Minghui Shi, Jianbin Wang, Li Yu
Summary: This study finds that monocytes in chicken embryos foster a pro-angiogenic microenvironment by depositing migrasomes enriched in angiogenic factors before angiogenesis.
NATURE CELL BIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Sudheer K. Cheppali, Raviv Dharan, Roni Katzenelson, Raya Sorkin
Summary: In this study, a new tool for protein-membrane interaction studies was developed based on natural membranes. The method allows for the investigation of membrane proteins within a background of native membrane components while preserving their correct orientation.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Multidisciplinary Sciences
Qikun Yi, Xin Dai, Byung Min Park, Junhao Gu, Jiren Luo, Ri Wang, Cong Yu, Songzi Kou, Jinqing Huang, Richard Lakerveld, Fei Sun
Summary: Engineered living materials (ELMs) have gained attention for their unique properties and nonequilibrium thermodynamics. This study presents a strategy to assemble engineered Saccharomyces cerevisiae into self-propagating ELMs using ultrahigh-affinity protein/protein interactions. These materials have potential applications in chemical separation and biomedical fields.
Article
Multidisciplinary Sciences
Qiang Zheng, Mingzeng Peng, Zhuo Liu, Shuyu Li, Rongcheng Han, Han Ouyang, Yubo Fan, Caofeng Pan, Weiguo Hu, Junyi Zhai, Zhou Li, Zhong Lin Wang
Summary: This study developed a force mapping method based on a light nano-antenna array with the use of piezo-phototronic effect, achieving high-resolution dynamic mapping of cell-generated force. The methodology can be applied for fundamental study of cardiomyocyte behavior and provide valuable information for heart-related research.
Article
Biochemical Research Methods
Rob Ward, Sapna Ravindran, Mariela R. Otazo, Braden Cradock, Ebubekir Avci, Graeme Gillies, Christina Coker, Martin A. K. Williams
Summary: The advancement in technologies allowing probing of individual microscopic particles has opened up new experimental possibilities in colloidal systems. However, due to the probabilistic nature of the process, testing a large number of particle pairs is necessary under each set of experimental conditions to obtain meaningful data.
Article
Nanoscience & Nanotechnology
Wenqiang Li, Xia Wang, Jiaming Liu, Shuai Li, Nan Li, Huizhu Hu
Summary: Introducing rotational degree of control into conventional optical tweezers offers new possibilities in physics, optical manipulation, and life science. Previous rotational schemes relied on microsphere anisotropy, limiting their applications. This study demonstrates the first experimental realization of orbiting a homogeneous microsphere using angular momentum in a transversely rotating optical trap. This method enables the exploration of orbital-translational coupling and the creation of an ultra-stable micro-gyroscope.
Article
Chemistry, Multidisciplinary
Elham Babaei, Demelza Wright, Reuven Gordon
Summary: By combining fringe-field dielectrophoresis with nano aperture optical tweezers, single molecule analysis of proteins in an aqueous environment without modification is achieved. The trapping time for proteins is significantly faster when the counter electrode is positioned outside of the solution compared to the more common configuration inside the solution. These findings are a major advancement for the nanoaperture optical trapping technique for high-throughput protein analysis.
Article
Multidisciplinary Sciences
Serapion Pyrpassopoulos, Allison M. Gicking, Taylor M. Zaniewski, William O. Hancock, E. Michael Ostap
Summary: KIF1A is a highly efficient vesicle transport motor that plays a role in neurodegenerative diseases. Optical tweezers were used to study its ability to generate force and sustain it against hindering loads. The results showed that KIF1A exhibits higher force and attachment duration in a three-bead assay compared to a single-bead assay. It was also found that KIF1A has a faster reengagement rate with microtubules after detachment compared to KIF5B. These findings provide insights into the adaptations of KIF1A in transport under load.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Optics
Keren Zhalenchuck, Barak Hadad, Alon Bahabad
Summary: This article demonstrates the operation of an optical conveyor with the geometry of an Archimedes screw, allowing particles to be conveyed along any desired paraxial trajectory.
LASER & PHOTONICS REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Sk Alim, Sudheer K. Cheppali, Mikko Laitaoja, Thirumala Rao Talluri, Janne Janis, Musti J. Swamy
Summary: A major FnII family protein, DSP-1, was identified and isolated from donkey seminal plasma, showing homology to other mammalian seminal plasma proteins. The protein is heterogeneously glycosylated and contains multiple acetylations, with binding to choline phospholipids increasing its thermal stability. Further studies suggest that DSP-1 binding to sperm plasma membrane could be physiologically significant.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biochemistry & Molecular Biology
Sudheer K. Cheppali, Raviv Dharan, Raya Sorkin
Summary: Optical tweezers have been used to precisely measure forces and distances in biological processes. This review discusses the use of optical tweezers to study membranes, including the characterization of protein-membrane interactions, membrane curvature generation, and membrane fusion and fission.
JOURNAL OF MEMBRANE BIOLOGY
(2022)
Article
Chemistry, Physical
Asaf Shemesh, Avi Ginsburg, Raviv Dharan, Yael Levi-Kalisman, Israel Ringel, Uri Raviv
Summary: This study used state-of-the-art solution synchrotron time-resolved small-angle X-ray scattering to reveal the disassembly catastrophe of tubulin rings and oligomers and the effects of assembly conditions on the structures.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Nanoscience & Nanotechnology
Sudheer K. Cheppali, Raviv Dharan, Roni Katzenelson, Raya Sorkin
Summary: In this study, a new tool for protein-membrane interaction studies was developed based on natural membranes. The method allows for the investigation of membrane proteins within a background of native membrane components while preserving their correct orientation.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Physical
Asaf Shemesh, Nadiv Dharan, Avi Ginsburg, Raviv Dharan, Yael Levi-Kalisman, Israel Ringel, Uri Raviv
Summary: This study characterized the structural changes during the initial nucleation phase of dynamic tubulin and revealed an isodesmic assembly mechanism. They found that a significant amount of tubulin octamers accumulated before the appearance of two-dimensional nanostructures.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Multidisciplinary
Shahar Goren, Maayan Levin, Guy Brand, Ayelet Lesman, Raya Sorkin
Summary: Fibrous hydrogels are important components of soft animal tissues, supporting cellular functions and facilitating mechanical communication between cells. This study investigates force propagation in tensed fibrin hydrogels and finds that it becomes anisotropic, with a stronger response to perturbations perpendicular to the axis of tension. Furthermore, external tension can increase the range of force transmission.
Article
Multidisciplinary Sciences
Raviv Dharan, Yuwei Huang, Sudheer Kumar Cheppali, Shahar Goren, Petr Shendrik, Weisi Wang, Jiamei Qiao, Michael M. Kozlov, Li Yu, Raya Sorkin
Summary: The formation of migrasomes is a two-stage process. In the first stage, local swellings devoid of tetraspanin 4 form on retraction fibers due to membrane tension. In the second stage, tetraspanin 4 migrates to and binds onto these swellings, which grow and transform into migrasomes. Tetraspanin 4 recruitment is essential for migrasome growth and stabilization.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Dijin Xu, Weiqian Jiang, Lizhen Wu, Ryan G. Gaudet, Eui-Soon Park, Maohan Su, Sudheer Kumar Cheppali, Nagarjuna R. Cheemarla, Pradeep Kumar, Pradeep D. Uchil, Jonathan R. Grover, Ellen F. Foxman, Chelsea M. Brown, Phillip J. Stansfeld, Joerg Bewersdorf, Walther Mothes, Erdem Karatekin, Craig B. Wilen, John D. MacMicking
Summary: Understanding protective immunity to COVID-19 facilitates preparedness for future pandemics and combats new SARS-CoV-2 variants. PLSCR1 is identified as a potent cell-autonomous restriction factor against SARS-CoV-2 infection and other highly pathogenic coronaviruses. It interferes with viral entry and fusion, providing a potential target for antiviral strategies.