Journal
PLANT SCIENCE
Volume 322, Issue -, Pages -Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2022.111329
Keywords
Sea-island cotton; TCP transcription factor; Drought stress; Salinity; Abscisic acid
Categories
Funding
- Tianshan Innovation Team Plan [2020D14002]
- Xinjiang Uygur Autonomous Region Major Science and Technology Project [2021A02001-3]
- National Natural Science Foundation of China [U1903203]
- Xinjiang Uygur Autonomous Region Postgraduate Innovation Project [XJ2021G157]
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The GbTCP4 gene in sea-island cotton positively regulates drought and salt stress responses, making it a candidate gene for improving plant drought and salt tolerance.
TCP transcription factors play important regulatory roles in plant growth and development; however, their function in response to salt and drought stress in sea-island cotton (Gossypium barbadense) is unknown. Here, GbTCP4 expression was induced by abscisic acid (ABA), drought, and NaCl treatments. Under drought stress, compared to wild-type (WT) Arabidopsis, transgenic GbTCP4-overexpressing Arabidopsis showed increased seed germination rate, root length and survival rate; additionally, it was ABA-insensitive at the germination stage but ABA-sensitive at the seedling stage, showing reduced stomatal opening and ABA enrichment. Under salt stress, compared to WT Arabidopsis, transgenic GbTCP4-overexpressing Arabidopsis showed greater root length, survival rate, and SPAD value and lower malondialdehyde (MDA) content. Conversely, under drought or salt stress, virusinduced gene-silenced GbTCP4 cotton showed decreased root length, area and volume and increased MDA content and sensitivity to drought and salt stress compared with control cotton. RNA-seq and quantitative realtime PCR analyses showed that GbTCP4 affected the transcription levels of genes across multiple abiotic stressrelated metabolic pathways. Furthermore, GbTCP4 activated the transcription of GbUVR8 and GbbHLH130 by binding to their promoters. These results suggest that GbTCP4 positively regulates drought and salt stress responses and is a suitable candidate gene for improving plant drought and salt tolerance.
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