Journal
JOURNAL OF LEUKOCYTE BIOLOGY
Volume 100, Issue 5, Pages 855-864Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1189/jlb.2HI1115-525R
Keywords
disintegrin; adhesion; antigen presentation; cytoskeleton; costimulation
Categories
Funding
- U.S. National Institutes of Health [1SC2GM103741]
- Department of Defense [W911NF-14-1-0123]
- National Science Foundation [1428768]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1428768] Funding Source: National Science Foundation
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ADAM23 is a member of the brain macrophage-derived chemokine family. Structural homology of ADAM proteins suggests their function as integrin receptors. Previous studies have linked ADAM23 as a dominant contributor to brain development and cancer metastasis. The present studies now show that ADAM23 expression on DCs partially governs antigen-presentation capacities to responder CD4(+) T cells. With the use of RNAi approaches, knockdown of ADAM23 in murine BMDCs resulted in impaired T cell activation, proliferation, and cytokine production. Knockdown did not alter the maturation profile of DCs (i.e., costimulatory molecule expression or production of proinflammatory cytokines) but markedly impaired cognate T cell responses. There was a significant decrease in antigen-specific clonal expansion coupled with a global decrease in Th cytokine production. Impaired early activation and proliferation did not alter/skew the balance of Th polarization but significantly depressed total levels of IL-2, IFN-gamma, IL-4, and IL-17 cytokine production in CD4(+) T cells primed by ADAM23 knockdown versus control DCs. Finally, neutralizing antibodies targeting the alpha(v)beta(3) integrin receptors resulted in similar phenotypes of impaired CD4(+) T cell responses. Taken together, these studies show a novel role of ADAM23 in governing DC antigen presentation to cognate CD4(+) T cells.
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