Article
Plant Sciences
Liyao Su, Tian Zhang, Bin Yang, Tianyu Dong, Xiaoyu Liu, Yibo Bai, Hui Liu, Jingsong Xiong, Yan Zhong, Zong-Ming (Max) Cheng
Summary: This study analyzed the evolutionary history, expansion and contraction patterns, and changes in interaction relationships of TIR1/AFB and AUX/IAA genes. The results showed that gene duplication and whole-genome duplication contributed to the expansion of the AUX/IAA gene family, while TIR1/AFB gene duplicates were lost after whole-genome duplication. Additionally, specific interactions and gene expression patterns were found to contribute to the functional diversification of TIR1/AFB and AUX/IAA.
Article
Agronomy
Justyna Prusinska, Veselina Uzunova, Paul Schmitzer, Monte Weimer, Jared Bell, Richard M. Napier
Summary: This article studies the mechanisms and effects of auxin herbicides in plants, and compares the differences between different classes of auxin herbicides.
PEST MANAGEMENT SCIENCE
(2023)
Article
Plant Sciences
Zhiwen Qiao, Hongliang Li, Xiaofei Wang, Xinglong Ji, Chunxiang You
Summary: In this study, eight MdAFBs were identified in apple, and a comprehensive analysis was conducted to investigate their characteristics and functions. The results showed that MdAFB1 interacted with three auxin repressor proteins and responded to osmotic and salt stress. Overexpression of MdAFB1 increased osmotic and salt resistance in apple calli, and ectopic expression of MdAFB1 enhanced osmotic and salt tolerance in Arabidopsis. This study provided insights into the identification and functions of auxin receptor genes in apple in mediating osmotic and salt stress.
HORTICULTURAL PLANT JOURNAL
(2023)
Review
Plant Sciences
Lukas Fiedler, Jiri Friml
Summary: Plants have developed multiple receptor systems and signal transduction cascades to respond to the hormone auxin. However, there is still a lack of comprehensive understanding of the various mechanisms involved in auxin signaling. The rapid and slow effects of auxins on plant physiology and development have been a historical controversy in the field. Recent progress has shed light on these unknowns and may have broader implications beyond auxin signaling.
CURRENT OPINION IN PLANT BIOLOGY
(2023)
Review
Plant Sciences
Linlin Qi, Jiri Friml
Summary: cAMP is a versatile second messenger in mammalian signaling pathways, but its role in plants is not well understood. Recent research has discovered that TIR1/AFB auxin receptors in plants have adenylate cyclase activity and are important for auxin signaling. This article summarizes the established cAMP signaling pathways in mammalian cells, discusses the turbulent history of plant cAMP research, and highlights the major progress and unresolved questions. The current paradigm of auxin signaling is also briefly reviewed to provide background for the discussion on the AC activity of TIR1/AFB auxin receptors and its potential role in transcriptional auxin signaling, as well as the impact of these discoveries on plant cAMP research in general.
Review
Biochemistry & Molecular Biology
Zipeng Yu, Feng Zhang, Jiri Friml, Zhaojun Ding
Summary: Auxin, one of the most widely studied phytohormones, has been a hot topic in plant biology. The advances in understanding auxin signaling, including perception, rapid responses, transcriptional and non-transcriptional branches, and epigenetic regulation, have been highlighted. Feedback inhibition mechanisms and non-canonical signaling have also been explored. Further identification of auxin signaling components and their regulation will deepen our understanding of how auxin signals regulate plant growth and development at the cellular level.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lorena Rodriguez-Paez, Charmina Aguirre-Alvarado, Norma Oviedo, Veronica Alcantara-Farfan, Edgar E. Lara-Ramirez, Guadalupe Elizabeth Jimenez-Gutierrez, Joaquin Cordero-Martinez
Summary: This study investigated the influence of polyamines on sperm channels and found that polyamines interacted with sodium and potassium channels, potentially affecting the membrane potential of sperm. Additionally, alkalization of pHi during capacitation suggested that increased levels of cAMP may activate NHEs, which is supported by in silico analysis of interactions with polyamines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Marcelo R. A. de Figueiredo, Anita Kuepper, Jenna M. Malone, Tijana Petrovic, Ana Beatriz T. B. de Figueiredo, Grace Campagnola, Olve B. Peersen, Kasavajhala V. S. K. Prasad, Eric L. Patterson, Anireddy S. N. Reddy, Martin F. Kubes, Richard Napier, Franck E. Dayan, Christopher Preston, Todd A. Gaines
Summary: A 27-nucleotide deletion removing nine amino acids in the degron tail of the auxin coreceptor Aux/IAA2 (SoIAA2) is associated with 2,4-D resistance in a Sisymbrium orientale weed population. The deletion reduces binding to TIR1 and increases dissociation rates, resulting in resistance to synthetic auxin herbicides.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Environmental Sciences
Wei Guo, Wei Wang, Weiwei Zhang, Wei Li, Yichen Wang, Sufen Zhang, Jianghai Chang, Qingfu Ye, Jay Gan
Summary: This study elucidated the mechanistic understanding of PAA enantioselectivity using model PAA compounds, showing that the R enantiomer exhibited preferential binding to TIR1-IAA7, enhanced plant absorption, and greater translocation capability compared to the S enantiomer, ultimately explaining the mechanisms driving PAA enantioselectivity.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biophysics
Tongle Liu, Yuan Rao, Wenfeng Zhou, Wei Zhuang, Lei Ge, Rijia Lin, Wenxia Zhang, Jinglan Wu, Chenjie Zhu, Hanjie Ying
Summary: By modifying graphene oxide with PDA/PEI and utilizing the affinity between metal ions and AC's His-tags, directional immobilization of AC was successfully achieved. The modified GO increases amino density for affinity group decoration and adjusts the interaction between AC and the support. This method can improve enzyme activity and immobilization efficiency.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Plant Sciences
Wenchao Du, Yang Lu, Qiang Li, Shuangxia Luo, Shuxing Shen, Na Li, Xueping Chen
Summary: This article focuses on the important role of TIR1/AFB proteins in plant responses to diverse abiotic and biotic stress. It highlights the studies that have shed light on the mechanisms by which TIR1/AFB proteins are regulated at the transcriptional and post-transcriptional levels, as well as the downstream pathways regulated by the TIR1/AFB family in abiotic or biotic stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Nutrition & Dietetics
Kota Shiozawa, Misato Maeda, Hsin-Jung Ho, Tomoko Katsurai, Md Zakir Hossain Howlader, Kimiko Horiuchi, Yumi Sugita, Yusuke Ohsaki, Afifah Zahra Agista, Tomoko Goto, Michio Komai, Hitoshi Shirakawa
Summary: The study found that supplementation of biotin increased testosterone levels in mice and testis-derived cells. This effect was mediated by the activation of adenylate cyclase and protein kinase A. These findings suggest that biotin may have the potential to improve age-related male syndromes associated with declining testosterone production.
Article
Biochemistry & Molecular Biology
Jun Ma, Qiang Li, Lei Zhang, Sen Cai, Yuanyuan Liu, Juncheng Lin, Rongfeng Huang, Yongqiang Yu, Mingzhang Wen, Tongda Xu
Summary: High auxin induces callus by promoting H3K36 histone methylation, which requires the involvement of the methyltransferase SDG8. During callus induction, auxin stimulates the expression of SDG8 through transcriptional regulation. SDG8 then deposits H3K36me3 modifications on the loci of callus-related genes, leading to their transcriptional activation. This study reveals the coordination between auxin signaling and epigenetic regulation during callus formation.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Agronomy
Long Jiang, Zhongnan Li, Xiaoming Yu, Chunbo Liu
Summary: This study analyzed the structural characteristics and gene expression patterns of Aux/IAA transcription factors in maize, revealing the important regulatory role of the Aux/IAA gene family in the process of plant growth hormone regulation. The evolutionary tree suggested that this gene family is both ancient and extensive, mainly functioning in the nucleus, with some genes highly expressed under high osmotic pressure conditions, potentially involved in maize's resistance to osmotic stress.
Article
Biology
Junpei Yamashita, Yuji Nishiike, Thomas Fleming, Daichi Kayo, Kataaki Okubo
Summary: In a study using the medaka model, it was found that two neuropeptides, Pacap and Vip, exhibit sexually dimorphic expression in the preoptic area, with estrogen playing a role in maintaining their sex-dependent activity. This study highlights the importance of alternative preoptic neuropeptides in regulating pituitary hormones in males and females.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Lanxin Li, Huihuang Chen, Saqer S. Alotaibi, Ales Pencik, Maciek Adamowski, Ondrej Novak, Jiri Friml
Summary: This study investigates the mechanism of rapid alkalinization factors (RALFs) in regulating plant growth. It is found that RALF1 inhibits the growth of Arabidopsis thaliana roots by inducing apoplast alkalinization, which is mediated by the receptor FERONIA (FER) through net H+ influx. Additionally, the study reveals cross-talk between RALF1 and auxin signaling pathways, where RALF1 up-regulates auxin biosynthesis to regulate root growth. The biphasic action of RALF1 on growth allows plants to respond rapidly to environmental stimuli and reprogram growth and development in the long term.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Kosuke Fukui, Kazushi Arai, Yuka Tanaka, Yuki Aoi, Vandna Kukshal, Joseph M. Jez, Martin F. Kubes, Richard Napier, Yunde Zhao, Hiroyuki Kasahara, Ken-ichiro Hayashi
Summary: The phytohormone auxin, indole-3-acetic acid (IAA), plays a significant role in plant development. The research characterizes a potent GH3 inhibitor called kakeimide that selectively targets IAA-conjugating GH3 proteins. Chemical knockdown experiments demonstrate that auxin turnover is rapid, suggesting that both auxin biosynthesis and inactivation dynamically regulate auxin homeostasis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Agronomy
Jiaqi Xu, Xudong Liu, Richard Napier, Liyao Dong, Jun Li
Summary: Halauxifen-methyl disrupts IAA homeostasis and stimulates overproduction of ethylene and ABA by inducing the overexpression of genes involved in their biosynthesis, ultimately leading to plant death.
Article
Agronomy
Justyna Prusinska, Veselina Uzunova, Paul Schmitzer, Monte Weimer, Jared Bell, Richard M. Napier
Summary: This article studies the mechanisms and effects of auxin herbicides in plants, and compares the differences between different classes of auxin herbicides.
PEST MANAGEMENT SCIENCE
(2023)
Review
Plant Sciences
Sireethorn Tungsirisurp, Rachel O'Reilly, Richard Napier
Summary: Our understanding of the quantities and distributions of phytohormones in different cells and tissues heavily relies on labor-intensive mass spectrometry techniques. However, the use of genetically modified biosensors has enabled the spatial and temporal quantification of phytohormones within cells, but information about their distribution between cells is still limited. In this review, we explore the potential of nucleic acid aptamers as a promising biosensing platform for detecting and quantifying molecules with high affinity and specificity. We discuss the selection, sequencing analysis, and evaluation of affinity and specificity in DNA aptamer technology, focusing on previously developed DNA aptamers targeting various plant molecules. We also propose potential applications of these tools for intracellular and intercellular quantification of molecules of interest in planta.
TRENDS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jonas Becker, Roberto Terracciano, Gokhan Yilmaz, Richard Napier, C. Remzi Becer
Summary: Glycopolymers have potential for biomedical applications by exploiting interactions between carbohydrates and lectins. They can be used for targeted drug delivery to specific cells through their specific recognition capabilities. However, a challenge in glycopolymers research is the specificity of recognition to receptors binding to the same sugar unit. In this study, a method for producing glycopolymers with a defined tacticity based on click chemistry was presented.
Review
Physics, Multidisciplinary
S. Boccaletti, P. De Lellis, C. I. del Genio, K. Alfaro-Bittner, R. Criado, S. Jalan, M. Romance
Summary: The beauty, richness, and harmony of a complex system depend on how its elementary components interact. Network Science has emerged as a multidisciplinary field to model distributed systems and uncover the underlying mechanisms. However, networks only capture pairwise interactions, while real-world systems often involve collective actions at the group level. Hypergraphs and simplicial complexes provide a more comprehensive representation to capture multi-component interactions. This report summarizes the literature on the structure and dynamics of hypergraphs and simplicial complexes and highlights their importance in real-world distributed systems.
PHYSICS REPORTS-REVIEW SECTION OF PHYSICS LETTERS
(2023)
Article
Plant Sciences
Ilias El Houari, Petr Klima, Alexandra Baekelandt, Paul E. E. Staswick, Veselina Uzunova, Charo I. I. Del Genio, Ward Steenackers, Petre I. I. Dobrev, Roberta Filepova, Ondrej Novak, Richard Napier, Jan Petrasek, Dirk Inze, Wout Boerjan, Bartel Vanholme
Summary: Chemical inhibitors are commonly used for functional gene characterization, but the non-specific effects in complex biological settings are often overlooked. In this study, we found that piperonylic acid (PA), an inhibitor of C4H, competes with GH3.6 and increases IAA levels in plants, affecting other GH3-conjugated phytohormones. Our results highlight the importance of considering the interference of chemical inhibitors with endogenous processes in studies.
Article
Agronomy
Paula Sinigaglia Angonese, Andrew Rerison Silva de Queiroz, Liana Sinigaglia Angonese, Filipi Mesquita Machado, Richard Napier, Catarine Markus, Carla A. Delatorre, Aldo Merotto Jr
Summary: Previous studies have characterized the resistant plants of Sumatran fleabane with rapid necrosis symptoms after applying 2,4-D. This study aimed to investigate the causes of variability in the occurrence of the rapid necrosis and found that environmental conditions, plant growth stage, and herbicide interactions can interfere with the symptoms. The herbicides dicamba and triclopyr effectively controlled resistant plants, especially when sprayed at the initial growth stages, providing insights into the mechanisms and causes of herbicide symptomology and performance variability.
Review
Plant Sciences
Linlin Qi, Jiri Friml
Summary: cAMP is a versatile second messenger in mammalian signaling pathways, but its role in plants is not well understood. Recent research has discovered that TIR1/AFB auxin receptors in plants have adenylate cyclase activity and are important for auxin signaling. This article summarizes the established cAMP signaling pathways in mammalian cells, discusses the turbulent history of plant cAMP research, and highlights the major progress and unresolved questions. The current paradigm of auxin signaling is also briefly reviewed to provide background for the discussion on the AC activity of TIR1/AFB auxin receptors and its potential role in transcriptional auxin signaling, as well as the impact of these discoveries on plant cAMP research in general.
Article
Plant Sciences
Yinwei Zeng, Inge Verstraeten, Hoang Khai Trinh, Robin Lardon, Sebastien Schotte, Damilola Olatunji, Thomas Heugebaert, Christian Stevens, Mussa Quareshy, Richard Napier, Sara Paola Nastasi, Alex Costa, Bert De Rybel, Catherine Bellini, Tom Beeckman, Steffen Vanneste, Danny Geelen
Summary: This study reveals the molecular processes involved in the initiation of adventitious root (AR) formation along the hypocotyl of Arabidopsis seedlings exposed to light. The results show that AR induction by HYSPARIN involves nuclear TIR1/AFB and plasma membrane TMK auxin signaling, as well as multiple downstream LR development genes. Additionally, SAUR19, OFP4, and AGC2 are identified as novel regulators of AR formation.
Article
Plant Sciences
Vladimir Skalicky, Ioanna Antoniadi, Ales Pencik, Ivo Chamrad, Rene Lenobel, Martin F. Kubes, Marek Zatloukal, Asta Zukauskaite, Miroslav Strnad, Karin Ljung, Ondrej Novak
Summary: Auxin and cytokinin show subcellular distribution in Arabidopsis cells, forming hormone gradients. This finding provides important insights into the mechanisms of plant growth and development.