Journal
CARBOHYDRATE RESEARCH
Volume 411, Issue -, Pages 6-14Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2014.12.014
Keywords
Lactobacillus helveticus MB2-1; Exopolysaccharides; Anti-colon cancer activity; Structural characterization; Methylated analysis; Nuclear magnetic resonance spectroscopy
Funding
- National Natural Science Foundation of China [31371807, 31201422]
- Fundamental Research Funds for the Central Universities [KYZ201419]
- Key Projects in the National Science & Technology Pillar Program [2013BAD18B01-4]
- High-Tech Research and Development Program of China [2011AA100903]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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The present study aimed at investigating the potential anti-colon cancer activity of three purified exopolysaccharides fractions (LHEPS-1, LHEPS-2 and LHEPS-3) from the Lactobacillus helveticus MB2-1. The experimental evidence showed that LHEPS-1 significantly inhibited cell proliferation of human colon cancer Caco-2 cells in both time-and concentration-dependent manners. In contrast, no significant improvements of the inhibitory effects of LHEPS-2 and LHEPS-3 on Caco-2 cells were observed with increasing sample concentrations or prolonged incubation time. Furthermore, the structure of LHEPS-1 was elucidated using methylated analysis, gas chromatography-mass spectroscopy (GC-MS) and nuclear magnetic resonance spectroscopy (NMR), including one-and two-dimensional nuclear magnetic resonance (1D and 2D NMR). Results indicated that the LHEPS-1 consisted of a decasaccharide repeating unit with the following structure (n approximate to 122): Our results suggested that the LHEPS-1 produced by L. helveticus MB2-1 might be suitable for using as natural anti-colon cancer drugs and functional foods ingredients. (C) 2015 Elsevier Ltd. All rights reserved.
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