Journal
CARBOHYDRATE POLYMERS
Volume 134, Issue -, Pages 808-816Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.06.088
Keywords
Sea cucumber; Fucoidan; Structure; ES-MS-MS; NMR
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Funding
- National Science Foundation of China [31301417, 31201304]
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Sea cucumber fucoidan is a major bioactive component of sea cucumber. The structures of fucoidans have significant influences on their biological activities. The present study clarified the delicate structure of a fucoidan from Pearsonothuria graeffei. Fucoidan was obtained after papain digestion and purified by ion chromatography. The carbohydrate sequence of fucoidan was firstly determined by negative-ion electrospray tandem mass spectrometry (ES-MS) with collision-induced dissociation of the oligosaccharide fragments, which were obtained by mild acid hydrolysis, and completed by NMR for assignment of the anomeric conformation. It was unambiguously identified as a tetrasaccharide repeating unit with a backbone of [-> 3Fuc (2S, 4S) alpha 1 -> 3Fuc alpha 1 -> 3Fuc (4S) alpha 1 -> 3 Fuc#7x10#]n. The glycosidic bonds between the non-sulfated and 2,4-O-disulfated fucose residues were selectively cleaved, and highly ordered oligosaccharide fragments with a tetrasaccharide repeating unit were obtained. The highly 4-O- and 2, 4-di-O-sulfated polysaccharide deserves further developments for Pharmacia use. (C) 2015 Elsevier Ltd. All rights reserved.
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