Article
Chemistry, Multidisciplinary
Jay Min Lim, Rahul Tevatia, Ravi F. Saraf
Summary: This study found that small nucleic acids have a significant impact on the limit of quantification in qPCR analysis, primarily due to the length of the PCR product. Therefore, it is crucial to consider the size of the nucleic acids and the length of the PCR product during primer design for qPCR.
Article
Entomology
Yue Cao, Baishu Li, Naizhong Chen, Ding Yang, Li Li, Tao Liu
Summary: In this study, seven internal reference genes of Bactrocera dorsalis under various quarantine treatments were screened, with G6PDH and RPL-32 identified as the most stable genes for analyzing phytosanitary treatment in B. dorsalis. This research provides a foundation for further understanding the resistance mechanisms of B. dorsalis to abiotic stresses.
Article
Chemistry, Analytical
Ian T. Hull, Enos C. Kline, Gaurav K. Gulati, Jack Henry Kotnik, Nuttada Panpradist, Kamal G. Shah, Qin Wang, Lisa Frenkel, James Lai, Joanne Stekler, Barry R. Lutz
Summary: The study presents a novel quantitative detection method for HIV DNA or RNA using a combination of recombinase polymerase amplification and lateral flow detection technologies, allowing for rapid and accurate testing in low-resource healthcare settings, with the potential to improve the quality of HIV treatment.
ANALYTICAL CHEMISTRY
(2022)
Article
Virology
Yiting Wang, Xuewen Li, Yifei Wang, Zheyu Tu, Jiancheng Xu, Junqi Pan, Qi Zhou
Summary: Performance evaluation of two COVID-19 detection kits (Maccura kit and Sansure kit) was conducted in real clinical work. The study found differences in the cycle threshold (Ct) values generated by the two kits, but they presented a linear positive correlation. Recalculating the cut-off values for the Maccura kit reduced false positives. It is recommended to use two kits and optimize their performance to improve testing accuracy.
Article
Agriculture, Dairy & Animal Science
Yaping Liu, Wanting Zhong, Shuo Feng, Ziteng Tang, Yingzhi Zhang, Lianzhong Ai, Zhiqiang Xiong
Summary: In this study, gene expression variance in B. animalis was analyzed using RNA sequencing, and stable reference genes were identified. Through integration of multiple analysis algorithms, rplD and atpA were determined as stable reference genes. Using these two genes for colony counting, accurate results were obtained in fermented skim milk. This study provides a foundation for accurate analysis of colony counting of B. animalis in dairy foods.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Virology
Yuka Horio, Mototada Shichiri, Yuji Isegawa
Summary: A novel method was developed to evaluate mRNA transcription activity of influenza RdRp without the use of ultracentrifugation and radioisotopes. The method showed specificity and sensitivity in quantifying mRNA synthesis, even at low levels, and was able to demonstrate the inhibition of mRNA synthesis by an RdRp inhibitor. This new approach will facilitate further research on identifying compounds that inhibit viral mRNA transcription activity of RdRp.
Article
Parasitology
Gereon Schares, Majda Globokar Vrhovec, Mareen Tuschy, Maike Joeres, Andrea Baerwald, Bretislav Koudela, Jitender P. Dubey, Pavlo Maksimov, Franz J. Conraths
Summary: The 529-bp repeat of H. hammondi can be used to establish a quantitative real-time PCR assay, with excellent specificity and sensitivity. This assay is a valuable diagnostic tool for epidemiological and cell biological studies on H. hammondi and related parasites.
PARASITES & VECTORS
(2021)
Article
Biology
Darkhan Kanayev, Diana Abilmazhenova, Ilyas Akhmetollayev, Aliya Sekenova, Vyacheslav Ogay, Arman Kulyyassov
Summary: In vivo biotinylation using wild-type and mutant biotin ligases is widely used in the study of cellular proteomes. The commercial availability of high-efficiency biotinylated protein purification kits and their compatibility with LC-MS/MS protocols are the main factors for the choice of biotin ligases. However, quantifying the expression of these enzymes in various samples can be difficult. This study used real-time qRT-PCR to quantify the expression of BirA fusions and found high expression levels in models of pluripotency transcription factor interactions.
Article
Public, Environmental & Occupational Health
E. Murillo-Zamora, O. Mendoza-Cano, I Delgado-Enciso, C. M. Hernandez-Suarez
Summary: The study identified factors predicting severe symptomatic laboratory-confirmed SARS-CoV-2 reinfection, including increasing age, comorbidities (such as obesity, asthma, type 2 diabetes mellitus), and previous severe laboratory-confirmed coronavirus disease 2019 history.
Article
Biology
Seda Arslan Tuncer, Hakan Ayyildiz, Mehmet Kalayci, Taner Tuncer
Summary: The study introduces a 25-depth CNN model called Scat-NET using scattergram images to detect COVID-19, achieving an overall accuracy of 92.4%. It is proposed as an alternative to CT scans and a secondary test for patients with negative RT-PCR results to improve detection accuracy.
COMPUTERS IN BIOLOGY AND MEDICINE
(2021)
Article
Multidisciplinary Sciences
Waleed M. M. El-Sayed, Alli L. Gombolay, Penghao Xu, Taehwan Yang, Youngkyu Jeon, Sathya Balachander, Gary Newnam, Sijia Tao, Nicole E. Bowen, Tomas Bruna, Mark Borodovsky, Raymond F. Schinazi, Baek Kim, Yongsheng Chen, Francesca Storici
Summary: The distribution of rNMPs in different organelle genomes of Chlamydomonas reinhardtii reveals specific biases and patterns, reflecting the varying ATP levels present in the algal cells. Researchers identified the orthologous genes for all three subunits of the RNase H2 enzyme in the algal organelles using different tools and protocols.
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Pharmacology & Pharmacy
A. Yuwono Soeroto, R. Antartika, N. Nauli Asriputri, H. Suryadinata, B. Andriyoko
Summary: This study aimed to determine the association between RT-PCR Ct value and COVID-19 clinical severity in the second week of illness. The results showed no significant difference in Ct values among different severity groups, suggesting that Ct value is clinically insignificant in terms of association with the severity of COVID-19 in the second week of illness.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2022)
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Chemistry, Analytical
Padideh Mohammadyousef, Miltiadis Paliouras, Mark A. Trifiro, Andrew G. Kirk
Summary: In response to the need for rapid infectious pathogen detection, researchers have been working on adapting PCR for POC applications. This paper introduces a compact plasmonic PCR thermocycler for fast DNA amplification using photothermal heating with gold nanorods and a VCSEL. An ultrasensitive real-time amplicon detection strategy is also reported, based on monitoring UV absorption of the PCR sample cycle-by-cycle. This system allows for high detection sensitivity and does not require specific probes or fluorophores.
