Article
Multidisciplinary Sciences
Yan-Xia Liu, Wen -Juan Li, Rui-Kai Zhang, Sheng-Nan Sun, Zhen-Chuan Fan
Summary: Certain ciliary signaling proteins are transported from the tip to the base of cilia via retrograde intraflagellar transport (IFT), facilitated by the BBSome and ARL13. The interaction between ARL13GTP and BBS3GTP at the ciliary tip plays a crucial role in properly coupling the BBSome with signaling cargo. This understanding provides mechanistic insights into the defects seen in hedgehog signaling in humans with ARL13b and BBS3 mutations.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Albert J. Bae, Raheel Ahmad, Eberhard Bodenschatz, Alain Pumir, Azam Gholami
Summary: This study investigates the propulsion mechanism of micro-particles and reveals that the asymmetric sideways attachment of the flagellum to the bead significantly affects the rotational velocity of the micro-swimmer. Surprisingly, an increase in the size of the cargo leads to an increase in some components of the velocity of the bead. These findings are important for the fabrication of synthetic, bio-actuated medical micro-robots for targeted drug delivery.
Article
Biology
Karl F. Lechtreck, Yi Liu, Jin Dai, Rama A. Alkhofash, Jack Butler, Lea Alford, Pinfen Yang
Summary: Intraflagellar transport (IFT) plays a crucial role in protein transportation into flagella, but the interaction between IFT trains and diverse proteins remains largely unknown. This study reveals that ARMC2/PF27 is necessary for IFT of radial spokes in Chlamydomonas, providing insights into the assembly process of flagella.
Review
Biology
Xiaoyu Tian, Huijie Zhao, Jun Zhou
Summary: The BBSome is an octameric protein complex that regulates ciliary transport and signaling. Mutations in BBSome subunits are closely associated with ciliary defects and lead to ciliopathies, notably Bardet-Biedl syndrome. An improved understanding of BBSome-mediated biological events and molecular mechanisms is expected to help advance the development of diagnostic and therapeutic approaches for BBSome-related diseases.
Article
Multidisciplinary Sciences
Rui-Kai Zhang, Wei-Yue Sun, Yan-Xia Liu, Emma Y. Zhang, Zhen-Chuan Fan
Summary: This study reveals the mechanism of signal protein export from cilia via the RABL2-ARL3 cascade-mediated outward BBSome transition zone diffusion pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Tooba Quidwai, Jiaolong Wang, Emma A. Hall, Narcis A. Petriman, Weihua Leng, Petra Kiesel, Jonathan N. Wells, Laura C. Murphy, Margaret A. Keighren, Joseph A. Marsh, Esben Lorentzen, Gaia Pigino, Pleasantine Mill
Summary: Intraflagellar transport (IFT) is a key mechanism for delivering cargo within cilia. The protein WDR35 plays a role in transporting ciliary membrane cargo necessary for cilia elongation, showing homology with COPI coatomer subunits and involvement in vesicle transport. Mutations in WDR35 disrupt this process and lead to defects in cilia formation and protein delivery.
Article
Physics, Fluids & Plasmas
Smitha Maretvadakethope, Yongyun Hwang, Eric E. Keaveny
Summary: Cilia and flagella play important roles in cell locomotion, embryonic development, and fluid transport. This study examines the coordination and phase transition mechanisms of cilia motion through simulations. The findings show that the motion pattern of cilia can be controlled by adjusting a single parameter.
PHYSICAL REVIEW FLUIDS
(2022)
Article
Biochemistry & Molecular Biology
Michael W. Stuck, Weng Man Chong, Jung-Chi Liao, Gregory J. Pazour
Summary: Primary cilia are essential for development and health, and dysfunction is associated with a range of human pathologies. The small GTPase Rab34 was identified as a key protein in ciliary formation, particularly important for internal assembly pathway. Additionally, it was found that IMCD3 cells can utilize the internal assembly pathway for ciliogenesis.
Article
Biology
Adria Razzauti, Patrick Laurent
Summary: Cilia are sensory organelles protruding from cell surfaces that can release extracellular vesicles (EVs) in some organisms. These EVs are formed by ciliated sensory neurons, with release from both the cilia tip and periciliary membrane compartment. The formation and release of EVs help maintain cilia composition.
Article
Multidisciplinary Sciences
Markus Masek, Christelle Etard, Claudia Hofmann, Andreas J. Huelsmeier, Jingjing Zang, Masanari Takamiya, Matthias Gesemann, Stephan C. F. Neuhauss, Thorsten Hornemann, Uwe Straehle, Ruxandra Bachmann-Gagescu
Summary: The study identifies the role of Bbs1 in lipid homeostasis of photoreceptor outer segments, which may contribute to vision loss in patients with Bardet-Biedl syndrome.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Komal Sharma, Irina Sizova, Sibaji K. Sanyal, Girdhar K. Pandey, Peter Hegemann, Suneel Kateriya
Summary: In this study, two important motifs, VXPX and LKNE, were identified at the C-terminus of ChR1. VXPX is a ciliary targeting sequence in animals, while LKNE is a well-known SUMOylation motif. The study showed that VMPS of ChR1 is essential for interaction with GTPase CrARL11 and revealed the presence of SUMO motifs in the C-terminus of putative ChR1s from green algae. Additionally, the study demonstrated the potential role of SUMOylation in modulating ChR1 protein in Chlamydomonas.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Xiumin Yan, Yidong Shen
Summary: Primary cilia are important sensory organelles in cells, with intraflagellar transport essential for their assembly, maintenance, and function. Recent studies have highlighted the critical regulatory roles of three ciliary Rab-like small GTPases in ciliary BBSome transport.
Article
Biology
Yan-Xia Liu, Bin Xue, Wei-Yue Sun, Jenna L. Wingfield, Jun Sun, Mingfu Wu, Karl F. Lechtreck, Zhenlong Wu, Zhen-Chuan Fan
Summary: Research has shown that BBS3 promotes the exit of PLD from cilia via BBSome, providing a regulatory mechanism for the removal of ciliary signaling proteins from cilia.
Article
Chemistry, Multidisciplinary
Xiau Jeong Teng, Wei Ming Ng, Wai Hong Chong, Derek Juinn Chieh Chan, Rohimah Mohamud, Boon Seng Ooi, Chen Guo, Chunzhao Liu, JitKang Lim
Summary: The transport behavior of a microswimmer changes significantly before and after cargo loading, leading to decreased swimming velocity and the emergence of helical motion. The location of cargo loading has a great influence on the swimming behavior of the microswimmer, with the microswimmer showing different swimming characteristics when cargo is loaded at different positions.
Article
Biochemistry & Molecular Biology
Inna A. Nikonorova, Juan Wang, Alexander L. Cope, Peter E. Tilton, Kaiden M. Power, Jonathon D. Walsh, Jyothi S. Akella, Amber R. Krauchunas, Premal Shah, Maureen M. Barr
Summary: Extracellular vesicles (EVs) can mediate intercellular communication by carrying protein and RNA cargo. C. elegans is a tractable animal system for studying and identifying EV cargo. The study found that EVs in C. elegans also carry RNA, suggesting a potential role for EVs in extracellular RNA-based communication.
Article
Food Science & Technology
De-Mei Meng, Xiao-Min Yang, Xue-Qing Sun, Lei Cheng, Zhen-Chuan Fan
Summary: Mytichitin-A, expressed in Pichia pastoris, shows great potential as a preservative for fresh pork by effectively inhibiting bacterial growth and improving meat quality. It exhibits good pH and thermal stability, resistance to protease digestion and amino acid interference.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2021)
Article
Biology
Yan-Xia Liu, Bin Xue, Wei-Yue Sun, Jenna L. Wingfield, Jun Sun, Mingfu Wu, Karl F. Lechtreck, Zhenlong Wu, Zhen-Chuan Fan
Summary: Research has shown that BBS3 promotes the exit of PLD from cilia via BBSome, providing a regulatory mechanism for the removal of ciliary signaling proteins from cilia.
Article
Cell Biology
Jenna L. Wingfield, Betlehem Mekonnen, Ilaria Mengoni, Peiwei Liu, Mareike Jordan, Dennis Diener, Gaia Pigino, Karl Lechtreck
Summary: The study utilized two-color imaging of fluorescent protein-tagged IFT components to show that IFT-A and IFT-B proteins from a given anterograde train usually return in the same set of retrograde trains. Additionally, concurrent turnaround was typical for IFT-B proteins and the IFT dynein subunit D1 bLIC-GFP, but severance was also observed.
JOURNAL OF CELL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Wei-Yue Sun, Bin Xue, Yan-Xia Liu, Rui-Kai Zhang, Rong-Chao Li, Wen Xin, Mingfu Wu, Zhen-Chuan Fan
Summary: LZTFL1 maintains BBSome dynamics in cilia by controlling BBSome recruitment to the basal body and its reassembly at the ciliary tip, thus regulating ciliary signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Karl F. Lechtreck, Yi Liu, Jin Dai, Rama A. Alkhofash, Jack Butler, Lea Alford, Pinfen Yang
Summary: Intraflagellar transport (IFT) plays a crucial role in protein transportation into flagella, but the interaction between IFT trains and diverse proteins remains largely unknown. This study reveals that ARMC2/PF27 is necessary for IFT of radial spokes in Chlamydomonas, providing insights into the assembly process of flagella.
Review
Cell Biology
Peiwei Liu, Ying Liu, Jun Zhou
Summary: Cilia are surface-exposed organelles that provide motility and sensory functions for cells, and it is widely believed that mechanosensation can be mediated through cilia. Polycystin-1 and -2 (PC-1 and PC-2, respectively) are transmembrane proteins that can localize to cilia; however, the molecular mechanisms by which polycystins contribute to mechanosensation are still controversial.
JOURNAL OF CELL SCIENCE
(2023)
Article
Cell Biology
Yan-Xia Liu, Rui-Kai Zhang, Zhen-Chuan Fan
Summary: BBSome is a key component for the export of signaling proteins in cilia. IFT27 binds its partner IFT25 to form the IFT25/27 complex, which is necessary for BBSome reassembly. The interaction between IFT25/27 and the IFT-B subcomplex core IFT-B1 is enhanced by GTP-loading onto IFT27. The presence of IFT25/27 at the ciliary tip facilitates BBSome-dependent PLD export, regardless of the nucleotide state of IFT27.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yan-Xia Liu, Wen -Juan Li, Rui-Kai Zhang, Sheng-Nan Sun, Zhen-Chuan Fan
Summary: Certain ciliary signaling proteins are transported from the tip to the base of cilia via retrograde intraflagellar transport (IFT), facilitated by the BBSome and ARL13. The interaction between ARL13GTP and BBS3GTP at the ciliary tip plays a crucial role in properly coupling the BBSome with signaling cargo. This understanding provides mechanistic insights into the defects seen in hedgehog signaling in humans with ARL13b and BBS3 mutations.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Shuang Sun, Zhaoyang Xu, Haijie Hu, Manxi Zheng, Liang Zhang, Wei Xie, Lei Sun, Peiwei Liu, Tianliang Li, Liangran Zhang, Min Chen, Xueliang Zhu, Min Liu, Yunfan Yang, Jun Zhou
Summary: This study identified a toxin called alveolysin produced by Bacillus cereus that can disrupt cell junctions in intestinal epithelium by increasing CD59 and CFAP100 proteins. These findings reveal the mechanism by which B. cereus causes intestinal diseases and systemic infections, and propose new strategies for prevention and treatment.
Article
Multidisciplinary Sciences
Rui-Kai Zhang, Wei-Yue Sun, Yan-Xia Liu, Emma Y. Zhang, Zhen-Chuan Fan
Summary: This study reveals the mechanism of signal protein export from cilia via the RABL2-ARL3 cascade-mediated outward BBSome transition zone diffusion pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Chan Zhang, Rui-Kai Zhang, Ying Feng, Sheng-Nan Sun, Zhen-Chuan Fan
Summary: In this study, a fish-derived antimicrobial peptide (AMP), CATH_BRALE, was expressed in Chlamydomonas reinhardtii as a recombinant peptide with stable expression and broad-spectrum antibacterial activity. It showed strong thermostability and pH stability, and did not exhibit toxicity on different cell lines. The results demonstrate the potential of using C. reinhardtii as a host for producing biologically active CATH_BRALE.
PROCESS BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Hui Wang, Ying Feng, Hadiatullah Hadiatullah, Zhen-Chuan Fan
Summary: Researchers expressed antimicrobial peptides Mytichitin-CB and Hispidalin in Chlamydomonas reinhardtii and created a chimeric peptide called MCH. MCH demonstrated high efficiency in inhibiting bacterial growth and displayed strong resistance to temperature, pH, and proteases, while showing low toxicity towards erythrocytes and human kidney cells.
PROCESS BIOCHEMISTRY
(2023)
