Article
Multidisciplinary Sciences
Panjing Yin, Weiwei Su, Ting Li, Ling Wang, Jianying Pan, Xiaoqi Wu, Yan Shao, Huabin Chen, Lin Lin, Yang Yang, Xiulin Cheng, Yanbing Li, Yaobin Wu, Chun Zeng, Wenhua Huang
Summary: This study proposed a modular hydrogel-based bioink containing microsphere-embedded chondrocytes for 3D printing multi-scale scaffolds. The gelatin methacryloyl (GelMA)/alginate microspheres were prepared using a microfluidic approach and the chondrocytes remained viable after being frozen and resuscitated. The modular hydrogel bioink could be printed using the gel-in-gel 3D bioprinting strategy to fabricate the scaffolds, which showed good cell proliferation and differentiation in vitro and biocompatibility in vivo. These findings suggest that this modular hydrogel-based bioink has potential applications in articular cartilage tissue engineering.
Article
Nanoscience & Nanotechnology
Jian Wang, Liaoran Zhang, Jiaxue Zhu, Jianhua Gu, Xiang Wang, Hairong Tao
Summary: CUR-loaded chitosan nanoparticles and HA-CUR-loaded chitosan nanoparticles were synthesized for the treatment of osteoarthritis. The results showed that HA-CUR-loaded chitosan nanoparticles can restrict inflammation and chondrocyte apoptosis.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yi Wang, Qiuping Deng, Qinghua Zheng, Ruyan Li, Baoqing Liu, Xiaoli Mo, Jiahong Zhou, Jian Shen
Summary: This study prepared nanoparticles composed of polydopamine and quercetin for potential osteoarthritis treatment. The nanoparticles not only serve as drug carriers but also function as scavengers for reactive oxygen species. Modified hyaluronic acid was cross-linked onto the nanoparticles to provide joint protection and lubrication. The synthesized composite nanomaterials demonstrated good biocompatibility and the ability to regulate chondrocyte function.
Article
Biochemistry & Molecular Biology
Morgane Bourmaud, Mylene Zarka, Romain Le Cozannet, Pascale Fanca-Berthon, Eric Hay, Martine Cohen-Solal
Summary: The study found that Rubus idaeus extracts have a protective effect on chondrocyte metabolism and cartilage loss, reducing chondrocyte hypertrophy and cartilage breakdown. The extract also prevented the loss of proteoglycan and inhibited the expression of the NITEGE neoepitope. In an inflammatory environment, Rubus idaeus extracts can also reduce the expression of proinflammatory factors.
Article
Biochemistry & Molecular Biology
Annica Proehl, Milijana Batinic, Said Alkildani, Michael Hahn, Milena Radenkovic, Stevo Najman, Ole Jung, Mike Barbeck
Summary: The study showed that the new hyaluronate containing bone substitute material has comparable bone healing capacity to the control xenogeneic BSM, with no significant differences in bone formation or inflammatory tissue responses. Both materials were fully biocompatible and allowed for gradual osteoconductive bone regeneration without differences in tissue reactions compared to a sham operation group.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Rebecca Galla, Sara Ruga, Silvio Aprile, Sara Ferrari, Arianna Brovero, Giorgio Grosa, Claudio Molinari, Francesca Uberti
Summary: This study demonstrated the efficacy of a new high molecular weight plant-derived hyaluronic acid (HA) called GreenIuronic (R) in maintaining joint homeostasis and preventing harmful processes of osteoarthritis. It was found that GreenIuronic (R) can cross the intestinal barrier and has a chemical-biological profile that supports specific chondrocyte functions. Additionally, GreenIuronic (R) can modulate the molecular mechanism responsible for preventing and restoring cartilage degradation. These findings suggest that oral administration of GreenIuronic (R) could be a beneficial therapeutic strategy for osteoarthritis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Chih-Hao Chen, Hao-Hsi Kao, Yen-Chen Lee, Jyh-Ping Chen
Summary: In this study, a thermosensitive hyaluronic acid-g-poly(N-isopropylacrylamide) copolymer was synthesized and found to have sol-to-gel phase transition temperatures suitable for use as a scaffold for chondrocyte delivery. In vitro cell culture experiments showed that cells could proliferate in the polymer scaffold with full retention of cell viability and exhibit the phenotypic morphology of chondrocytes. In vivo studies confirmed the formation of ectopic cartilage when the polymer was used as a cell delivery vehicle. These findings suggest the promising potential of the thermosensitive hydrogel in cartilage tissue engineering applications.
Article
Biochemistry & Molecular Biology
Stefania Cannito, Valeria Bincoletto, Cristian Turato, Patrizia Pontisso, Maria Teresa Scupoli, Giorgia Ailuno, Ilaria Andreana, Barbara Stella, Silvia Arpicco, Claudia Bocca
Summary: This study proposes the use of HA- and PEGylated-liposomes as potential treatments for hepatocellular carcinoma (HCC). The up-regulation of CD44 in HCC patients and a murine model suggests the involvement of this receptor in HCC progression. The experimental results show that HA-liposomes are more easily internalized by cells that over-express CD44, while PEG-modified liposomes are rapidly taken up by macrophages and induce M1 polarization in THP1 cells.
Review
Biochemistry & Molecular Biology
Antony Hoarau, Myriam Polette, Christelle Coraux
Summary: Hyaluronic acid (HA) is a major component of the extracellular matrix with anti-inflammatory and immunomodulatory functions in the lungs. The size of HA determines its pro-inflammatory or anti-inflammatory effects. The interactions between HA and hyaladherins play a role in HA activities. LMW-HA is involved in pulmonary innate immunity by activating various cells, making it a potential therapeutic target for inflammatory respiratory diseases.
Article
Polymer Science
Tsuneo Tatara
Summary: The study investigated the effects of albumin and hydroxyethyl starch (HES) on solute permeation through sodium hyaluronic acid (NaHA) solutions, showing that albumin had longer relative permeation time compared to HES.
Article
Chemistry, Applied
Vignesh K. Rangasami, Sumanta Samanta, Vijay Singh Parihar, Kenta Asawa, Keying Zhu, Oommen P. Varghese, Yuji Teramura, Bo Nilsson, Jons Hilborn, Robert A. Harris, Oommen P. Oommen
Summary: This study focused on utilizing self-assembled nanoparticles to combine the anti-inflammatory drug dexamethasone with the chemotherapeutic agent doxorubicin and deliver them to cancer cells, showing different effects of dexamethasone on different cancer cell lines and demonstrating the reduction of deleterious side effects by encapsulating doxorubicin within the nanoparticles.
CARBOHYDRATE POLYMERS
(2021)
Article
Hematology
James C. Garmey, Jack M. Hensien, Daniel B. Harmon, Victoria Osinski, Chantel McSkimming, Melissa A. Marshall, Jens W. Fischer, Maria Grandoch, Coleen A. McNamara, Angelina Misiou
Summary: The deficiency of transcription factor Id3 leads to metabolic dysfunction in visceral obesity by promoting the accumulation of hyaluronic acid (HA) in adipose tissue and increasing the number of proinflammatory B2 cells, contributing to adipose tissue inflammation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Veterinary Sciences
Elena De Angelis, Roberta Saleri, Paolo Martelli, Lisa Elviri, Annalisa Bianchera, Carlo Bergonzi, Marta Pirola, Roberta Romeo, Melania Andrani, Valeria Cavalli, Virna Conti, Ruggero Bettini, Benedetta Passeri, Francesca Ravanetti, Paolo Borghetti
Summary: The study demonstrates that 3D printed CH scaffolds have good porosity for colonization and synthetic activity of chondrocytes. Enrichment with hyaluronic acid provides a more stable chondrocyte phenotype. The use of 3D CH scaffolds with PL instead of FBS in the medium results in increased gene expression of relevant ECM components.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Medicine, Research & Experimental
Tingting Xiao, Wei Hu, Yu Fan, Mingwu Shen, Xiangyang Shi
Summary: The study developed a novel nanosystem integrated with macrophages for tumor therapy. By encapsulating hyaluronic acid nanogels with polypyrrole photothermal agent and anticancer drug doxorubicin, tumor homing and combination photothermo-chemotherapy were achieved. The results showed that the hybrid nanosystem has good targeting capability and enhanced antitumor therapy effect.
Article
Biochemistry & Molecular Biology
Christoph Bauer, Lukas B. Moser, Vivek Jeyakumar, Eugenia Niculescu-Morzsa, Daniela Kern, Stefan Nehrer
Summary: This study investigated the effect of GC, HA, and a mixture of both on cytokine-treated chondrocytes in vitro. Results showed that adding HA or GC/HA increased metabolic activity of chondrocytes, reduced the side effects of GC supplementation, and decreased the release of pro-inflammatory cytokines.
Article
Orthopedics
Vivek Jeyakumar, Florian Halbwirth, Eugenia Niculescu-Morzsa, Christoph Bauer, Hannes Zwickl, Daniela Kern, Stefan Nehrer
Article
Biochemistry & Molecular Biology
Vivek Jeyakumar, Eugenia Niculescu-Morzsa, Christoph Bauer, Zsombor Lacza, Stefan Nehrer
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Orthopedics
Christoph Bauer, Hakan Goecerler, Eugenia Niculescu-Morzsa, Vivek Jeyakumar, Christoph Stotter, Ivana Toth, Thomas Klestil, Friedrich Franek, Stefan Nehrer
JOURNAL OF ORTHOPAEDIC RESEARCH
(2019)
Article
Engineering, Biomedical
B. Stojanovic, C. Bauer, C. Stotter, T. Klestil, S. Nehrer, F. Franek, M. Rodriguez Ripoll
ACTA BIOMATERIALIA
(2019)
Article
Engineering, Mechanical
Hakan Goecerler, Bernhard Pfeil, Friedrich Franek, Christoph Bauer, Eugenia Niculescu-Morzsa, Stefan Nehrer
INDUSTRIAL LUBRICATION AND TRIBOLOGY
(2020)
Article
Orthopedics
Christoph Stotter, Bojana Stojanovic, Christoph Bauer, Manel Rodriguez Ripoll, Friedrich Franek, Thomas Klestil, Stefan Nehrer
JOURNAL OF ORTHOPAEDIC RESEARCH
(2019)
Article
Orthopedics
Christoph Bauer, Christoph Stotter, Vivek Jeyakumar, Eugenia Niculescu-Morzsa, Bojana Simlinger, Manel Rodriguez Ripoll, Thomas Klestil, Friedrich Franek, Stefan Nehrer
Summary: CoCr ions concentration-dependently reduced metabolic activity and induced early and late apoptosis in articular chondrocytes. Expression of chondrocyte-specific genes was affected by CoCr ions, with an increase in SOX9 expression and a decrease in COL2A1 expression. Exposure to CoCr ions also caused a release of pro-inflammatory cytokines.
Article
Multidisciplinary Sciences
Christoph Stotter, Christoph Bauer, Bojana Simlinger, Manel Rodriguez Ripoll, Friedrich Franek, Thomas Klestil, Stefan Nehrer
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Article
Orthopedics
Christoph Bauer, Hakan Goecerler, Eugenia Niculescu-Morzsa, Vivek Jeyakumar, Christoph Stotter, Thomas Klestil, Friedrich Franek, Stefan Nehrer
Summary: Inflammation affects the biomechanical properties of cartilage under stress by increasing the coefficient of friction, decreasing proteoglycan content, and upregulating the expression of degradative enzyme genes in chondrocytes. However, there were no significant differences in cartilage-specific gene expression and metabolic activity between treated and untreated groups. In conclusion, treatment with cytokines results in structural changes in cartilage proteoglycan content and increased coefficient of friction during biotribological tests.
Article
Biochemistry & Molecular Biology
Vivek Jeyakumar, Nedaa Amraish, Eugenia Niculescu-Morsza, Christoph Bauer, Dieter Pahr, Stefan Nehrer
Summary: Tissue engineering strategies can promote bone regeneration by stimulating osteogenesis route, but the insufficiency of vasculature integration from host tissue remains a challenge. This study demonstrates that a hybrid biomaterial incorporating CD-ECM/SF scaffolds can enhance bone regeneration by priming hBMSC's into the late hypertrophic stage in vitro for ECO-mediated bone tissue regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lukas B. Moser, Christoph Bauer, Vivek Jeyakumar, Eugenia-Paulina Niculescu-Morzsa, Stefan Nehrer
Summary: The study found that in vitro, hyaluronic acid can enhance the attachment and branched appearance of chondrocytes, reducing the chondrotoxic effects of local anesthetics and providing an alternative approach for treating patients with knee osteoarthritis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Letter
Infectious Diseases
Matthias Pilecky, Stephan Harm, Christoph Bauer, Jennifer Zottl, Robert Emprechtinger, Tanja Eichhorn, Claudia Schildboeck, Michael Ecker, Martin Willheim, Viktoria Weber, Jens Hartmann
JOURNAL OF INFECTION
(2022)
Article
Biochemistry & Molecular Biology
Christoph Bauer, Lukas B. Moser, Vivek Jeyakumar, Eugenia Niculescu-Morzsa, Daniela Kern, Stefan Nehrer
Summary: This study investigated the effect of GC, HA, and a mixture of both on cytokine-treated chondrocytes in vitro. Results showed that adding HA or GC/HA increased metabolic activity of chondrocytes, reduced the side effects of GC supplementation, and decreased the release of pro-inflammatory cytokines.
Article
Multidisciplinary Sciences
Christoph Bauer, Eugenia Niculescu-Morzsa, Stefan Nehrer
Article
Biotechnology & Applied Microbiology
Vivek Jeyakumar, Eugenia Niculescu-Morzsa, Christoph Bauer, Zsombor Lacza, Stefan Nehrer
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2017)