Article
Multidisciplinary Sciences
Amy L. Bogue, Warunya Panmanee, Cameron T. McDaniel, Joel E. Mortensen, Edwin Kamau, Luis A. Actis, Jay A. Johannigman, Michael J. Schurr, Latha Satish, Nalinikanth Kotagiri, Daniel J. Hassett
Summary: The novel biocide AB569, composed of acidified nitrite and ethylenediaminetetraacetic acid, demonstrated bactericidal activity against multi-drug resistant Acinetobacter baumannii and Acinetobacter spp. AB569 treatment can prevent the bacteria from gaining an early growth advantage during infection and lead to extensive bactericidal activity upon subsequent exposures.
Article
Chemistry, Multidisciplinary
Ruohan Wei, Xuemei Yang, Han Liu, Tongyao Wei, Sheng Chen, Xuechen Li
Summary: The study demonstrates that chemically synthesized Pse-CRM197 conjugates can stimulate high immune responses and provide a new alternative for controlling infections caused by multidrug-resistant A. baumannii.
ACS CENTRAL SCIENCE
(2021)
Article
Biology
Jaeeun Park, Misung Kim, Bora Shin, Mingyeong Kang, Jihye Yang, Tae Kwon Lee, Woojun Park
Summary: The modification of outer membrane charge induced by polymyxin B (PMB) in Acinetobacter baumannii leads to PMB-resistant variants and clinical isolates producing outer membrane vesicles (OMVs). Upregulation of the pmr operon and decreased membrane-linkage proteins result in enhanced biofilm formation and protection of susceptible bacteria from PMB, suggesting OMVs function as decoys against PMB. OMVs also protect the microbial community against PMB treatment in anaerobic conditions, indicating their potential clinical significance in antibiotic resistance.
Article
Multidisciplinary Sciences
Sachio Suzuki, Phawinee Subsomwong, Kouji Narita, Noriaki Kawai, Takahito Ishiai, Wei Teng, Rojana Sukchawalit, Akio Nakane, Sadatomo Tasaka, Krisana Asano
Summary: By comparing AbOMVs isolated under hypoxic and normoxic conditions, it was found that they have different protein profiles. While both AbOMVs had similar cytotoxic effects on bacteria, AbOMVs from normoxia induced higher levels of the cytokine TNF-alpha production and invasion of Staphylococcus aureus and Pseudomonas aeruginosa. On the other hand, AbOMVs from hypoxia significantly enhanced A. baumannii invasion into lung epithelial cells. This finding sheds light on the complex interactions between A. baumannii, coinfecting pathogens, and host cells mediated by OMVs, particularly the different pathogenic effects of AbOMVs under normoxic and hypoxic conditions.
Article
Biochemistry & Molecular Biology
Viviana Teresa Orlandi, Eleonora Martegani, Fabrizio Bolognese, Nicola Trivellin, Francesco Garzotto, Enrico Caruso
Summary: Antimicrobial photodynamic therapy (aPDT) is gaining attention as a promising tool for treating microbial infections and sanitizing environments. A study on a panel of photosensitizers found that dicationic diaryl-porphyrins show potential in efficiently photoinactivating Pseudomonas aeruginosa, including inhibiting biofilm formation. These findings suggest the potential applicability of dicationic diaryl-porphyrins in different fields for combating difficult-to-treat microbes like P. aeruginosa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Irfan Ahmad, Aftab Nadeem, Fizza Mushtaq, Nikola Zlatkov, Muhammad Shahzad, Anton V. Zavialov, Sun Nyunt Wai, Bernt Eric Uhlin
Summary: Acinetobacter baumannii is a common drug-resistant bacterial pathogen, and its biofilms and pili play important roles in antibiotic resistance and adherence to epithelial cells.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Multidisciplinary Sciences
Lucie Semenec, Amy K. K. Cain, Catherine J. J. Dawson, Qi Liu, Hue Dinh, Hannah Lott, Anahit Penesyan, Ram Maharjan, Francesca L. L. Short, Karl A. A. Hassan, Ian T. T. Paulsen
Summary: This study characterizes the genomes of A. baumannii and K. pneumoniae strains co-isolated from a single human lung infection, and investigates their interactions through various assays. The findings shed light on their effects on antimicrobial resistance and virulence during co-infection.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Ulrike Blaschke, Evelyn Skiebe, Gottfried Wilharm
Summary: Acinetobacter baumannii is a critical priority pathogen for new antibiotic development, and it displays surface motility through twitching and surface-associated mechanisms. Mutated genes involved in biosynthesis, stress metabolism, RNA modification, and competence impact different phenotypes in strain-specific and species-specific ways, highlighting the importance of comparative analysis in studying gene functions and motility mechanisms in A. baumannii.
CURRENT MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Deepa Dehari, Aiswarya Chaudhuri, Dulla Naveen Kumar, Rohit Patil, Mayank Gangwar, Sonam Rastogi, Dinesh Kumar, Gopal Nath, Ashish Kumar Agrawal
Summary: In this study, Acinetobacter baumannii (A. baumannii)-specific bacteriophages (BPAB & phi;1) were isolated and loaded into chitosan microparticles dispersed in a gel, showing therapeutic efficacy against MDR A. baumannii clinical strains. The isolated BPAB & phi;1 belonged to the Corticoviridae family with a burst size of 102.12 ± 2.65 PFUs per infected host cell. The developed formulation exhibited excellent antibiofilm eradication potential in vitro and effective wound healing after topical application.
Article
Genetics & Heredity
Bardya Djahanschiri, Gisela Di Venanzio, Jesus Distel, Jennifer F. Breisch, Marius Alfred Dieckmann, Alexander Goesmann, Beate Averhoff, Stephan Goettig, Gottfried Wilharm, Mario Feldman, Ingo Ebersberger
Summary: This study investigates the genetic basis of human-pathogen interaction in Acinetobacter calcoaceticus-baumannii (ACB) complex, a group of multi-drug resistant bacteria. By analyzing over 3,000 Acinetobacter genomes, the researchers identify evolutionarily stable gene clusters (ESGCs) that are associated with the pathogenic phenotype. These clusters reveal the metabolic pathways, transcriptional regulators, and carbon source utilization that allow the pathogens to adapt to their human host. The study also demonstrates that the ACB clade has the unique ability to utilize kynurenine, a substance produced by humans as part of the immune response. This research provides valuable insights for the development of novel therapeutic strategies.
Article
Multidisciplinary Sciences
Woo Cheol Lee, Sungjae Choi, Ahjin Jang, Kkabi Son, Yangmee Kim
Summary: The study focused on the crystal structures of the beta-ketoacyl-ACP reductase ApeQ produced by an APE gene cluster in Gram-negative bacteria. It was found that ApeQ plays a crucial role in APE biosynthesis and cannot be substituted by a similar enzyme from the FAS gene cluster. Structural and functional analysis revealed a positively charged arginine patch in ApeQ as the cognate ACP-binding site.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Kirti Upmanyu, Qazi Mohd. Haq, Ruchi Singh
Summary: The number of antibiotic-resistant Acinetobacter baumannii infections in hospital settings is increasing rapidly. The World Health Organization has identified carbapenem-resistant A. baumannii as a priority pathogen for drug discovery. In this study, we screened 400 compounds provided as a Pandemic Response Box to identify potential compounds with antibacterial and antibiofilm activity against A. baumannii. We found that compound MMV396785 showed promising in vitro efficacy against A. baumannii, inhibiting biofilm formation by 93% and eradicating pre-formed biofilms by 60-77.4%.
Article
Microbiology
Manuel Romero, Celia Mayer, Stephan Heeb, Krittanont Wattanavaekin, Miguel Camara, Ana Otero, Paul Williams
Summary: This study introduces a novel biofilm culture system, the rolling biofilm bioreactor (RBB), which allows for the investigation of bacterial biofilm architecture and biology. Using this system, the researchers discovered mushroom-shaped structures in mature Acinetobacter baumannii biofilms that had not been observed in other models. They also showed the impact of quorum sensing on biofilm differentiation, composition, and antibiotic tolerance. The study highlights the importance of understanding the mechanisms of mature biofilm development for developing new strategies to eradicate and remove biofilms.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Josephine Joy Hubloher, Lisa van der Sande, Volker Mueller
Summary: This study focused on the importance of Na+ homeostasis in Acinetobacter baumannii. Inactivation of the Mrp antiporter resulted in increased sensitivity to pH, Na+, and Li+, as well as energetic defects and impaired motility and survival under anoxic and dry conditions.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yasir Adil Jabbar Alabdali
Summary: The goal of this study was to characterize and detect class 1, 2, and 3 integrons in antibiotic-resistant A. baumannii strains collected from a clinical environment in Iraq. The presence of class 1 integrons was found to significantly contribute to the acquisition of multi-drug resistant phenotypes in A. baumannii isolates, and it can be used as a predictive biomarker for multi-drug resistance.
JOURNAL OF ANTIBIOTICS
(2022)
Article
Spectroscopy
Faezeh Fallahi, Atarodalsadat Mostafavinia, Zahranadia Sharifi, Leila Mohaghegh Shalmani, Abdollah Amini, Houssein Ahmadi, Hamidreza Omidi, Masoumeh Hajihosseintehrani, Sahar Bayat, Michael R. Hamblin, Sufan Chien, Mohammad Bayat
Summary: This study reports the effects of photobiomodulation (PBM) on diabetic adipose-derived stem cells (ADSCs) in vitro. The results showed that PBM significantly increased ATP levels and mitochondrial membrane potential (MMP), while decreasing reactive oxygen species (ROS) levels and the expression of PINK1 and PARKIN genes in diabetic-ADSCs. These findings suggest that PBM has the potential to improve the function and quality of mitochondrial diabetic-ADSCs, making it a promising approach for treating diabetic foot ulcers in diabetic patients.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Review
Biochemistry & Molecular Biology
Nasim Ebrahimi, Elmira Fardi, Hajarossadat Ghaderi, Sahar Palizdar, Roya Khorram, Reza Vafadar, Masoud Ghanaatian, Fatemeh Rezaei-Tazangi, Payam Baziyar, Amirhossein Ahmadi, Michael R. Hamblin, Amir Reza Aref
Summary: Targeted therapy is a new approach in cancer treatment, using drugs that specifically target proteins in cancer cells. Inhibiting these proteins can impede cancer progression. Understanding the structures, activation mechanisms, and functions of receptor tyrosine kinases (RTKs) has led to the development of targeted drug therapies, including RTK inhibitors (RTKIs), some of which have been used in clinical settings. This article discusses RTKs and their role in cancer treatment, as well as the potential effects of combination drug therapy for drug resistant cancers.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Irina Pereira, Carina Monteiro, Miguel Pereira-Silva, Diana Peixoto, Claudia Nunes, Salette Reis, Francisco Veiga, Michael R. Hamblin, Ana Claudia Paiva-Santos
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Review
Infectious Diseases
Amir Hossein Miri, Mojtaba Kamankesh, Mazda Rad-Malekshahi, Abbas Yadegar, Maryam Banar, Michael R. Hamblin, Ismaeil Haririan, Hamid Asadzadeh Aghdaei, Mohammad Reza Zali
Summary: In this article, the conventional antibiotic treatment regimens for Helicobacter pylori are described, including empirical, tailored, and salvage therapies. The reasons for treatment failure against H. pylori are discussed, followed by the latest advances in the use of probiotic bacteria against H. pylori infection. Finally, a polymeric bio-platform for efficient delivery of probiotics for H. pylori infection is proposed.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2023)
Article
Biochemical Research Methods
Clara Maria Cobra Branco Scontri, Flavio de Castro Magalhaes, Ana Paula Moraes Damiani, Michael R. Hamblin, Antonio Roberto Zamuner, Cleber Ferraresi
Summary: PBMt has a time- and dose-response effect to reduce glycemia in T2DM patients. The best dose is 100 J, which can lower blood glucose levels and persist until 12 hours. Combination with oral hypoglycemic medication can further improve glycemic control, but also increase sympathetic activation.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Biochemistry & Molecular Biology
Alexia Marques Silva, Laisa Bonafim Negri, Juliana Cristina Biazzotto, Sergio de Paula Machado, Jeimison Duarte Santos, Jorge Fernandes Nasser Batista, Pedro Ivo S. Maia, Victor Marcelo Deflon, Lusiane M. Bendhack, Michael R. Hamblin, Roberto S. da Silva
Summary: We synthesized cis-[Ru(bpy)2(NO2-kappa N)Ln-](n-1) and cis-[Ru(bpy)2(NO2-kappa O)L n -](n-1) by reacting cis-[Ru(bpy)2(H2O) Ln-](n-2) with sodium nitrite or conducting basic cis-[Ru(bpy)2NO(Ln-)](n-3) hydrolysis. Photolysis at the metal-ligand charge transfer band (MLCT) of the isomers released nitric oxide (NO) at different rates. We investigated endothelium-dependent vasodilation induced by cis-[Ru(bpy)2(4-pic)(NO2-kappa N)]+ and cis-[Ru (bpy)2(4-pic)(NO2-kappa O)]+ in aortic rings and found that the release of NO was three times faster for cis-[Ru(bpy)2(NO2-kappa O)Ln-](n-1) compared to cis-[Ru(bpy)2(NO2-kappa N)Ln-](n-1).
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Review
Microbiology
Weitao Zhu, Michael R. Hamblin, Xiang Wen
Summary: Rosacea is a chronic inflammatory skin disorder affecting the centrofacial region. Its etiology and treatment methods are still unclear. This review examines common bacterial species found in the skin and gut microbiota of rosacea patients and their role in pathogenesis. Additionally, it summarizes the impact of factors such as temperature and age on rosacea and reviews commonly used clinical treatment methods, including antibiotics and probiotics, along with their mechanisms and precautions.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Mohammad Varzandeh, Leila Sabouri, Vahid Mansouri, Maliheh Gharibshahian, Nima Beheshtizadeh, Michael R. Hamblin, Nima Rezaei
Summary: Radiosensitizers are compounds or nanostructures that enhance the effectiveness of ionizing radiation in killing cells. They increase the susceptibility of cancer cells to radiation-induced killing while minimizing damage to healthy tissues. This review discusses various nano-radiosensitizers and their combinations with other cancer therapies, highlighting the benefits, drawbacks, challenges, and future prospects.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Review
Neurosciences
James C. Lech, Matthew T. J. Halma, Adejoke O. Obajuluwa, Malcolm Baker, Michael R. Hamblin
Summary: The impact of lighting environment in educational institutions on student biology has not received much attention. The current lighting environment in schools is not conducive to student health and educational performance. Cost-effective interventions can significantly improve student health and educational attainment. We strongly recommend that educational institutions consider the lighting environment when designing educational programs.
ANNALS OF NEUROSCIENCES
(2023)
Article
Immunology
Mohammad Hasan Jafari Najaf Abadi, Fahimeh Abdi Abyaneh, Noushid Zare, Javad Zamani, Amirhossein Abdoli, Fatemeh Aslanbeigi, Michael R. Hamblin, Hossein Tarrahimofrad, Mohammadreza Rahimi, Seyed Mohammadreza Hashemian, Hamed Mirzaei
Summary: Currently, there is a lack of recombinant vaccine for preventing or treating Salmonella infection. In this study, a multi-epitope vaccine against Salmonella was designed using in silico approach. The vaccine was predicted to be non-allergenic and antigenic, and it could interact with human TLR5, potentially inducing an immune response for the prevention and treatment of Salmonella infection.
MICROBIAL PATHOGENESIS
(2023)
Article
Engineering, Biomedical
Fahimeh Ghasemi Moravej, Abdollah Amini, Reza Masteri Farahani, Samira Mohammadi-Yeganeh, Atarodalsadat Mostafavinia, Houssein Ahmadi, Hamidreza Omidi, Fatemehalsadat Rezaei, Latif Gachkar, Michael R. Hamblin, Sufan Chien, Mohammad Bayat
Summary: Diabetic wounds lead to chronic inflammation and the development of diabetic foot ulcers, which can cause amputation and death. This study examined the effect of photobiomodulation (PBM) plus allogeneic diabetic adipose tissue-derived stem cells (ad-ADS) on wound healing in type I diabetic rats. The results showed that the ad-ADS plus PBM treatment group had significantly better histological results compared to the other experimental groups.
LASERS IN MEDICAL SCIENCE
(2023)
Article
Engineering, Biomedical
Atousa Janzadeh, Fatemeh Ramezani, Sogol Yousefi, Michael R. Hamblin, Negin Mojarad, Farinaz Nasirinezhad
Summary: Neuropathic pain following spinal cord injury is a long-lasting condition that reduces the quality of life. Photobiomodulation therapy has shown some progress in treating this type of pain. In this study, two PBMT protocols were compared, and the 4-week protocol was found to be more effective in improving pain and disability. PBMT therapy modulates immune system function to reduce neuropathic pain and motor dysfunction.
LASERS IN MEDICAL SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Thomas Willmott, Gavin Humphreys, Krista Chappell-Jones, Mark Dickinson, Jian R. Lu, Michael R. Hamblin, Paul Dark, Andrew J. McBain
Summary: The antibacterial effects of a polychromatic light device designed for intravenous application were assessed in vitro. Exposure to a 60-min sequential light cycle comprising 365, 530, and 630 nm wavelengths resulted in small but statistically significant reductions in viable counts for Staphylococcus aureus, Klebsiella pneumoniae, and Escherichia coli. Bacterial inactivation occurred only with red (630 nm) light, possibly mediated by reactive oxygen species generated by excitation of haem groups.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Neurosciences
Margaret A. Naeser, Paula Martin, Michael D. Ho, Maxine H. Krengel, Yelena Bogdanova, Jeffrey A. Knight, Michael R. Hamblin, Andrea E. Fedoruk, Luke G. Poole, ChiaHsin Cheng, BangBon Koo
Summary: Chronic traumatic encephalopathy is preceded by traumatic encephalopathy syndrome with worsening cognition and behavior/mood disturbances. Transcranial photobiomodulation (tPBM) may promote improvements by increasing ATP and vasodilation. The study found that tPBM improved cognition and behavior/mood in ex-football players.
JOURNAL OF ALZHEIMERS DISEASE REPORTS
(2023)
Article
Dermatology
Sebastiaan J. W. van Kraaij, Michael R. Hamblin, Gisele Pickering, Bill Giannokopoulos, Hayet Kechemir, Moritz Heinz, Iva Igracki-Turudic, Yalcin Yavuz, Robert Rissmann, Pim Gal
Summary: This study evaluated the local effects of the FIRTECH patch on skin perfusion, microcirculation, and oxygenation. The results showed that the patch had a slight increase in skin perfusion, but not significant; the treated areas had significantly higher oxygen consumption and skin temperature compared to the untreated areas. The study also found that the FIRTECH patch was well tolerated and had a non-thermal vasodilatory effect.
EXPERIMENTAL DERMATOLOGY
(2023)
