Article
Virology
Ye Chen, Qibin Liao, Tianyue Chen, Yuchao Zhang, Weien Yuan, Jianqing Xu, Xiaoyan Zhang
Summary: Successful vaccines induce specific immune responses and protect against various viral and bacterial infections. Noninactivated vaccines, especially viral vector vaccines such as adenovirus and poxvirus vaccines, play a dominant role in the vaccine market due to their ability to replicate and proliferate in vivo. Maintaining vaccine stability, including antigenicity and infectivity, is a key challenge in human and livestock vaccination. Freeze-drying and cold chain transportation are typical methods to ensure vaccine stability, although resource constraints can limit distribution in underdeveloped areas. In this study, new freeze-drying formulations were developed to enhance virus vaccine storage times and maintain immunogenicity at ambient temperatures.
Review
Pharmacology & Pharmacy
Ji Young Yu, Piyanan Chuesiang, Gye Hwa Shin, Hyun Jin Park
Summary: Liposomes have potential as drug delivery systems, but issues such as self-aggregation, coalescence, flocculation, and precipitation may occur during formulation or storage. Post-processing techniques like freeze drying, spray drying, and spray freeze drying have been used to increase the stability of liposomes. The use of protective agents to overcome limitations of each process is also crucial.
Article
Chemistry, Multidisciplinary
Sofie Meulewaeter, Gust Nuytten, Miffy H. Y. Cheng, Stefaan C. De Smedt, Pieter R. Cullis, Thomas De Beer, Ine Lentacker, Rein Verbeke
Summary: Research finds that freeze-drying can enable storage of mRNA lipid nanoparticles (LNPs) at room temperature and higher temperatures, without the need for freezing. The study also reveals that a sufficient lipid to mRNA weight ratio is necessary to prevent mRNA leakage during freeze-drying, and phosphate and Tris are suitable buffers for lyophilization of mRNA LNPs. Furthermore, the transfection properties of optimally lyophilized mRNA LNPs can be maintained for at least 12 weeks at different temperatures.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Food Science & Technology
Benjamin Schmid, Sofia Navalho, Peter S. C. Schulze, Simon Van de Walle, Geert Van Royen, Lisa M. Schuler, Ines B. Maia, Carolina R. Bastos, Marie-Christin Baune, Edwin Januschewski, Ana Coelho, Hugo Pereira, Joao Varela, Joao Navalho, Alexandre Miguel Cavaco Rodrigues
Summary: Microalgae are a promising resource for food and feed markets, and the development of sustainable drying technologies is crucial for cost-effective preservation of high-quality biomass. This study tested a solar dryer as an alternative to freeze drying and assessed its effects on biomolecular quality parameters. The results showed that solar drying is a cost-effective and sustainable technology for preserving microalgal biomass for food and feed markets.
Article
Pharmacology & Pharmacy
Yu-Sheng Yu, Khaled AboulFotouh, Haiyue Xu, Gerallt Williams, Julie Suman, Chris Cano, Zachary N. Warnken, Kevin C. -W Wu, Robert O. Williams III, Zhengrong Cui
Summary: In this study, a model vaccine containing liposomal monophosphoryl lipid A, QS-21 adjuvant, and ovalbumin (OVA) was successfully transformed from a liquid to a dry powder using thin-film freeze-drying technology. The dry powder was then administered to the desired regions of the nasal cavity using Aptar Pharma's Unidose Powder Nasal Spray System (UDSP), demonstrating the feasibility of intranasal vaccination.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Eloize Silva Alves, Cintia Stefhany Ripke Ferreira, Paulo Ricardo Souza, Andressa Rafaella Silva Bruni, Matheus Campos Castro, Bruno Henrique Figueiredo Saqueti, Oscar Oliveira Santos, Grasiele Scaramal Madrona, Jesui Vergilio Visentainer
Summary: This study investigated the effects of maltodextrin and gum arabic on freeze-dried human milk (HM), aiming to improve its quality and solubility. The results showed that both coatings led to higher solubility and better stabilization of HM, with gum arabic demonstrating superior performance. The combination of gum arabic and maltodextrin also showed promising results, indicating their potential synergistic effects.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Matthew S. Gatto, Wided Najahi-Missaoui
Summary: Nanoparticles have advantages and limitations as drug carriers, and lyophilization can increase their stability and the shelf life of drug products, but improvements are still needed in various aspects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Joaquin Gomez-Estaca, Alba Perez-Garcia, Ailen Aleman, M. Carmen Gomez-Guillen, Pilar Montero
Summary: The study evaluated the role of alginate in the drying and rehydration process of liposomal suspensions, showing that alginate can protect liposomes and provide protection at different pH levels.
FOOD HYDROCOLLOIDS
(2021)
Article
Chemistry, Multidisciplinary
Jasper W. Dittmar, Michelle H. Teplensky, Michael Evangelopoulos, Lei Qin, Bin Zhang, Chad A. Mirkin
Summary: The stability of the liposome core is crucial for the effectiveness of liposome-based drugs. This study explores the impact of anchor chemistry on immunotherapeutic function and demonstrates that a stable anchor can enhance immune stimulation and improve therapeutic efficacy.
Review
Multidisciplinary Sciences
Yingshu Guo, Xiuping Cao, Xiaofei Zheng, S. K. Jahir Abbas, Juan Li, Weihong Tan
Summary: This review summarizes the recent advances and biological applications of nanocarriers based on nucleic acids and provides perspectives on future directions for growth in this field. Nanocarriers based on nucleic acids have controllable size and modifiability, making them highly promising for cancer-cell-specific targeting.
NATIONAL SCIENCE REVIEW
(2022)
Article
Entomology
Letlhogonolo Selaledi, Monnye Mabelebele
Summary: The study investigated the impact of different drying procedures on the chemical composition and body color of yellow mealworm larvae. Sun-dried mealworms showed changes in essential amino acids and color, while freeze and oven-dried mealworms had similar crude protein content. Despite color changes, sun-drying resulted in the same nutritional composition as freeze and oven drying, making them suitable for food and feed products.
Article
Engineering, Chemical
Banu Koc, Hilal Isleroglu, Izzet Turker
Summary: Adsorption isotherms of microencapsulated microbial transglutaminase (mTG) powder produced by various drying methods were studied. The results showed that mTG powder produced by ultrasonic spray-freeze-drying (USFD) method exhibited the best quality parameters and stability.
Article
Agronomy
Guo-Liang Jiang, William Townsend, Shuxin Ren
Summary: This study aimed to find a drying method suitable for the fast evaluation of edamame nutritional profiles. Fresh seed samples of 20 soybean genotypes were dried using freeze, low- and high-heat drying methods, and their amino acid contents were analyzed by near-infrared reflectance (NIR) technology. The results showed significant differences in amino acid contents between years and genotypes, as well as between drying methods for most amino acids.
Article
Food Science & Technology
Huangliang Zheng
Summary: Bacteriophages are widely used in food processing and storage as natural antibacterial additives. However, there is limited data on phage freeze-drying and the factors that inactivate phages during freeze-drying and storage are unknown. This study compared different lyoprotectants and found that only completely vitrified formulations (glucose, sucrose, and trehalose) could preserve phages during freeze-drying.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Pharmacology & Pharmacy
Silvia Voci, Agnese Gagliardi, Maria Cristina Salvatici, Massimo Fresta, Donato Cosco
Summary: The study investigated the effects of different media on the physico-chemical features of zein and gliadin nanoparticles, and characterized the best formulations using freeze-drying. The results showed that a 5% glucose solution was a feasible medium for their development. Cryoprotectants were used to prevent aggregation, with mannose playing a crucial role in the cryopreservation of gliadin nanosystems.
Article
Infectious Diseases
Safiullah Najem, Dorothee Eick, Johannes Boettcher, Annette Aigner, Mona Aboutara, Ines Fenner, Konrad Reinshagen, Ingo Koenigs
Summary: This study aimed to determine the prevalence of multidrug resistant organisms (MDRO) carriage in children at admission to a pediatric hospital in Hamburg and to identify associated risk factors. Results showed that MRGN carriage was higher than MRSA and VRE, and a risk-factor-based admission screening system was recommended.
Review
Pharmacology & Pharmacy
Priyanga Ranasinghe, Melisande L. Addison, James W. Dear, David J. Webb
Summary: Post-transcriptional gene silencing can be achieved through siRNA therapy, which uses synthetic short double-stranded RNA molecules to specifically target and degrade mRNA transcripts. This technology offers advantages such as broad targeting capabilities and long-lasting effects. However, challenges in terms of pharmacokinetics and pharmacodynamics have been addressed through chemical modification and delivery systems. Several siRNA therapies have been approved for clinical use, but further advancements are needed to target organs beyond the liver and reach special sites.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Engineering, Biomedical
Yijing Zhang, Jingchao Li, Kanyi Pu
Summary: Nanomedicines with stimuli-responsive capabilities have shown potential in cancer treatment. However, traditional nanomedicines often cause severe side effects due to unintended drug retention in non-diseased tissues. Dual-and multi-responsive nanomedicines, which integrate different responsive components, provide a safer and more effective drug release mechanism, leading to improved therapeutic efficacy and reduced toxicity. This article summarizes recent advances in precision cancer therapy using dual-and multi-responsive nanomedicines and discusses their design strategies, working mechanisms, and applications in chemotherapy, phototherapy, and immunotherapy.
Article
Biochemical Research Methods
Michael Karimov, Marlene Scherer, Heike Franke, Alexander Ewe, Achim Aigner
Summary: A three-dimensional tissue slice air-liquid interface (ALI) culture model was developed for nanoparticle testing, which showed clearer differences in transfection efficacy and cytotoxicity compared to 2D cell culture. This model allows for the evaluation of biological nanoparticle efficacy and biocompatibility in an intact tissue environment, providing a cost-effective and time-saving alternative.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Chemistry, Medicinal
Lukas Heyder, Phil M. M. Hochban, Corey Taylor, Florent Chevillard, Christof Siefker, Christian Iking, Hannes Borchardt, Achim Aigner, Gerhard Klebe, Andreas Heine, Peter Kolb, Wibke E. Diederich
Summary: In this study, fragment-sized hits binding to Pim-1 kinase were optimized using a combination of computational, synthetic, and crystallographic expertise. The resulting potent ligands target rarely-targeted regions of Pim-1 kinase with affinities in the nanomolar range. The combination of computational and experimental approaches successfully developed a novel molecular scaffold for inhibition of Pim-1 kinase, targeting specific surface regions that have been less frequently investigated.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Entomology
Nagalapura Ramakrishnappa Prasannakumar, Vala Keshava Rao, Nagendraiah Jyothi, Sarikonda Saroja, Ankanahalli Naranayanashetty Lokesha, Govindaraju Ramkumar
Summary: Aphids, thrips, whiteflies, mealy bugs, and mites can cause significant crop losses. This study found that extracts from the seeds of Annona squamosa and Sapindus mukorossi have insecticidal properties against these pests, making them potential botanicals for safe pest management in organic farming.
JOURNAL OF APPLIED ENTOMOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Shinan Geng, Mengqin Guo, Guiting Zhan, Dingwen Shi, Liyun Shi, Lu Gan, Yanbing Zhao, Xiangliang Yang
Summary: A NIR light-triggered dePEGylation/ligand-presenting strategy based on thermal decomposition of azo bonds is developed in this study. It overcomes the PEG dilemma and improves tumor targeting and drug delivery by enhancing cellular uptake and tumor accumulation.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Analytical
Maria Sokol, Ivan Gulyaev, Mariia Mollaeva, Sergey Kuznetsov, Vladimir Zenin, Maksim Klimenko, Nikita Yabbarov, Margarita Chirkina, Elena Nikolskaya
Summary: The combinatorial administration of vorinostat and genotoxic agents enhances their antitumoral action against tumors. To support this, a high-performance liquid chromatography method was developed for the simultaneous determination of doxorubicin and vorinostat in polymeric nanoparticles. The method was precise and accurate, and could be adopted for routine analysis of the drugs in nanoparticles.
JOURNAL OF SEPARATION SCIENCE
(2023)
Review
Urology & Nephrology
Verena Sailer, Gunhild von Amsberg, Stefan Duensing, Jutta Kirfel, Verena Lieb, Eric Metzger, Anne Offermann, Klaus Pantel, Roland Schuele, Helge Taubert, Sven Wach, Sven Perner, Stefan Werner, Achim Aigner
Summary: Androgen deprivation therapy is important in treating advanced prostate cancer, but there is a need for novel treatment approaches. It is crucial to critically assess current preclinical models in order to understand tumor progression and resistance better.
NATURE REVIEWS UROLOGY
(2023)
Review
Chemistry, Multidisciplinary
Anisha D'Souza, Saeideh Nozohouri, Benjamin S. Bleier, Mansoor M. Amiji
Summary: Nucleic acid-based therapeutic molecules have great potential for treating diseases in the CNS, but delivering them to the brain's target cells and sub-cellular compartments is a challenge. This review discusses the challenges and recent advances in cellular and sub-cellular delivery using various mechanisms and routes of administration.
PHARMACEUTICAL RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Thomas Foster, Michael Lewkowicz, Christina Quintas, Corina Mihaela Ionescu, Melissa Jones, Susbin Raj Wagle, Bozica Kovacevic, Elaine Y. M. Wong, Armin Mooranian, Hani Al-Salami
Summary: Hearing loss is a significant issue worldwide, and gene delivery and nanoencapsulation technology may provide effective means for treatment.
Article
Oncology
Luis Alberto Bravo-Vazquez, Natalia Frias-Reid, Ana Gabriela Ramos-Delgado, Sofia Madeline Osorio-Perez, Hania Ruth Zlotnik-Chavez, Surajit Pathak, Antara Banerjee, Anindya Bandyopadhyay, Asim K. Duttaroy, Sujay Paul
Summary: MicroRNAs and long non-coding RNAs play a crucial role in the development and progression of pancreatic cancer. Their dysregulation is associated with chemoresistance and tumor growth, and they have the potential to be used as biomarkers for accurate diagnosis and as therapeutic targets.
TRANSLATIONAL ONCOLOGY
(2023)
Review
Cell Biology
Abdul Fatah A. Samad, Mohd Farizal Kamaroddin
Summary: MicroRNA has the potential to regulate genetic pathways and has been explored as a therapeutic tool. However, there are challenges such as off-target effects, immunogenicity, and delivery platforms. Recently, innovative approaches have been developed to address these issues. Nevertheless, dosage remains an uncertainty for miRNA administration.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
Review
Materials Science, Biomaterials
Longyi Zhu, Jun Luo, Kewei Ren
Summary: Nucleic acid nanotechnology, with its high editability, easy synthesis, and modification, has emerged as a powerful tool in biosensing and nanomedicine. This review summarizes recent progress in the design and functional mechanisms of nucleic acid-based artificial nano-vectors, particularly for the delivery of exogenous siRNA and antisense oligonucleotides. Various types of DNA and RNA-based nanocarriers are introduced, along with enhanced targeting strategies to improve delivery efficacy. The potential challenges and outlook for nucleic acid-based artificial nanocarriers are also highlighted.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Biochemistry & Molecular Biology
Sewon Lim, Jinhyuk Park, Seung-Eun Chong, Sungwhan Kim, Yoonhwa Choi, So Hee Nam, Yan Lee
Summary: In this study, we substituted hydrophobic amino acids in the SAP(E) sequence with cysteine to enhance the cell penetrating activity and cargo conjugation. The cysteine substitution significantly improved the cell penetrating activity and showed lower co-localization with acidic endosomes or lysosomes compared to the positively-charged TAT. The cysteine handle of SAP(E)-7C allowed the conjugation and release of cargo drugs, demonstrating its potential as a useful module for CPP-based drug delivery.
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
