An Overview into Polyethylene Terephthalate (PET) Hydrolases and Efforts in Tailoring Enzymes for Improved Plastic Degradation

Plastic or microplastic pollution is a global threat affecting ecosystems, with the current generation reaching as much as 400 metric tons per/year. Soil ecosystems comprising agricultural lands act as microplastics sinks, though the impact could be unexpectedly more far-reaching. This is troubling as most plastic forms, such as polyethylene terephthalate (PET), formed from polymerized terephthalic acid (TPA) and ethylene glycol (EG) monomers, are non-biodegradable environmental pollutants. The current approach to use mechanical, thermal, and chemical-based treatments to reduce PET waste remains cost-prohibitive and could potentially produce toxic secondary pollutants. Thus, better remediation methods must be developed to deal with plastic pollutants in marine and terrestrial environments. Enzymatic treatments could be a plausible avenue to overcome plastic pollutants, given the near-ambient conditions under which enzymes function without the need for chemicals. The discovery of several PET hydrolases, along with further modification of the enzymes, has considerably aided efforts to improve their ability to degrade the ester bond of PET. Hence, this review emphasizes PET-degrading microbial hydrolases and their contribution to alleviating environmental microplastics. Information on the molecular and degradation mechanisms of PET is also highlighted in this review, which might be useful in the future rational engineering of PET-hydrolyzing enzymes.

Automated summary

Plastic and microplastic pollution is a global threat to ecosystems, with an annual production reaching up to 400 tons. Soil ecosystems, including agricultural lands, act as sinks for microplastics, but the impacts may be more far-reaching. Current methods to reduce PET waste are costly and potentially produce toxic secondary pollutants, highlighting the need for better remediation methods. Enzymatic treatments offer a promising approach, as enzymes can function under near-ambient conditions without the use of chemicals. This review focuses on PET-degrading microbial hydrolases and their contribution towards alleviating environmental microplastics, highlighting information on PET's molecular and degradation mechanisms.