Article
Immunology
Lifei Hou, Koichi Yuki
Summary: This study reveals the importance of CD11c in maintaining T cell survival by showing that CD11c deficiency leads to defects in T cell development in mice.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Geriatrics & Gerontology
Yan Li, Pei Chen, Hao Huang, Huiyu Feng, Hao Ran, Weibin Liu
Summary: This study found that all common DC subsets are present in the human thymus of various ages, with the density of pDCs gradually increasing with age, suggesting an additional role of pDCs in the thymus beyond central tolerance. The ratio of medulla to cortex area in the human thymus increases with age, while the densities of different DC subsets also show changes.
Review
Chemistry, Multidisciplinary
Ying Kong, Jiazhi Duan, Feng Liu, Lin Han, Gang Li, Chunhui Sun, Yuanhua Sang, Shuhua Wang, Fan Yi, Hong Liu
Summary: The application of physical signals in tissue engineering has the potential to regulate stem cell fate through nanostructure mediation. This method provides a new perspective for research and is expected to aid in the development of remote-controlled and wireless platforms to guide stem cell differentiation.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Oncology
Sergej Tomic, Andelija Petrovic, Nevena Puac, Nikola Skoro, Marina Bekic, Zoran Lj. Petrovic, Miodrag Colic
Summary: This study found that plasma-activated medium (PAM) induced immunogenic cell death in tumor cells and increased the immunogenicity of tumor lysates, enhancing the maturation potential of dendritic cells and promoting cytotoxic T cell responses. Compared to standard methods, this novel approach could be suitable for improving DC-based cancer immunotherapy.
Article
Multidisciplinary Sciences
Narges Aghaallaei, Advaita M. Dick, Erika Tsingos, Daigo Inoue, Eva Hasel, Thomas Thumberger, Atsushi Toyoda, Maria Leptin, Joachim Wittbrodt, Baubak Bajoghli
Summary: The study in medaka fish reveals that progenitors first enter a thymic niche favoring the development of gamma delta T cells, and the gradients of interleukin-7 and Ccl25a in the niche influence the lineage outcome. This previously unknown mechanism determines the proportion of alpha beta/gamma delta lineages within species.
Article
Microbiology
Sabrina Nicolo, Giorgio Mattiuz, Alberto Antonelli, Fabio Arena, Vincenzo Di Pilato, Tommaso Giani, Ilaria Baccani, Ann Maria Clemente, Giuseppe Castronovo, Michele Tanturli, Federico Cozzolino, Gian Maria Rossolini, Maria Gabriella Torcia
Summary: The study suggests that hypervirulent Klebsiella pneumoniae (Hv-Kp) strains can interfere with the function of dendritic cells (DCs) and the differentiation of T cells, possibly evading immune response and contributing to pathogen dissemination in immunocompetent hosts.
Article
Cell Biology
Hideyuki Nakashima, Keita Tsujimura, Koichiro Irie, Takuya Imamura, Cleber A. Trujillo, Masataka Ishizu, Masahiro Uesaka, Miao Pan, Hirofumi Noguchi, Kanako Okada, Kei Aoyagi, Tomoko Andoh-Noda, Hideyuki Okano, Alysson R. Muotri, Kinichi Nakashima
Summary: This study reveals the mechanism of MeCP2/miR-199a axis in regulating neural stem cell differentiation, further confirming the importance of BMP signaling in RTT brain development, and proposing the MeCP2/miR-199a/Smad1 axis as a potential therapeutic target for RTT.
Article
Allergy
Rafael Gras-Pena, Nichole M. Danzl, Mohsen Khosravi-Maharlooei, Sean R. Campbell, Amanda E. Ruiz, Christopher A. Parks, William Meng Suen Savage, Markus A. Holzl, Debanjana Chatterjee, Megan Sykes
Summary: This study developed a novel differentiation protocol to generate human thymic epithelial progenitors from human embryonic stem cells (hES-TEPs) and demonstrated their thymopoietic function in vivo. By incorporating hES-TEPs into a supportive thymic structure, the researchers enhanced human thymocyte development and increased the reconstitution of peripheral CD4+ naive T cells.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Oncology
Ansam Sinjab, Guangchun Han, Warapen Treekitkarnmongkol, Kieko Hara, Patrick M. Brennan, Minghao Dang, Dapeng Hao, Ruiping Wang, Enyu Dai, Hitoshi Dejima, Jiexin Zhang, Elena Bogatenkova, Beatriz Sanchez-Espiridion, Kyle Chang, Danielle R. Little, Samer Bazzi, Linh M. Tran, Kostyantyn Krysan, Carmen Behrens, Dzifa Y. Duose, Edwin R. Parra, Maria Gabriela Raso, Luisa M. Solis, Junya Fukuoka, Jianjun Zhang, Boris Sepesi, Tina Cascone, Lauren Averett Byers, Don L. Gibbons, Jichao Chen, Seyed Javad Moghaddam, Edwin J. Ostrin, Daniel Rosen, John Heymach, Paul Scheet, Steven M. Dubinett, Junya Fujimoto, Ignacio I. Wistuba, Christopher S. Stevenson, Avrum Spira, Linghua Wang, Humam Kadara
Summary: The study reveals the geospatial evolution of cell populations, states, and transcriptomic features in lung adenocarcinoma (LUAD) from normal regions to tumors. LUAD shows significant intratumor cell heterogeneity and transcriptional lineage-plasticity programs. T regulatory cell phenotypes increase near LUAD, while cytotoxic CD8(+) T cells and other cell types decrease in normal tissues.
Article
Cell Biology
Honglin Jia, Ru Zhang, Xiaoying Liang, Xiaofang Jiang, Qian Bu
Summary: This study suggests that miR-126 may play an important regulatory role in allergic rhinitis by positively regulating the expression of Treg cytokines and negatively regulating the expression of Th1 and Th2 cytokines, thus contributing to the imbalance of immune response in AR.
CELLULAR SIGNALLING
(2022)
Review
Engineering, Biomedical
Calvin Chang, Jerry Yan, Zhicheng Yao, Chi Zhang, Xiaowei Li, Hai-Quan Mao
Summary: This review focuses on the bioactivity and secretome of mesenchymal stem cells, as well as the engineering approaches to tune the MSC secretome. It also discusses the recent advances in biomaterials-based therapeutic strategies for delivering MSCs and MSC-derived secretome in extracellular vesicles, along with their advantages and challenges.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Immunology
Yesenia Brito-Perez, Rodrigo T. Camacho-Pacheco, Noemi Plazola-Camacho, Diana Soriano-Becerril, Irma A. Coronado-Zarco, Gabriela Arreola-Ramirez, Gabriela Gonzalez-Perez, Alma Herrera-Salazar, Julio Flores-Gonzalez, Mextli Y. Bermejo-Haro, Brenda G. Casorla-Cervantes, Ismael A. Soto-Lopez, Jessica Hernandez-Pineda, Claudia Sandoval-Montes, Sandra Rodriguez-Martinez, Ricardo Figueroa-Damian, Ismael Mancilla-Herrera
Summary: HEU newborns have lower percentages of differentiated Th-1, Th-2, Th-17, and CD4(+)CD25(++) T cells in their blood, but the intrinsic abilities of CD4(+) T cells to acquire a Th-1 profile are not affected by the adverse maternal milieu during development. Additionally, under Th-1 differentiation conditions, HEU newborns show higher percentages of CD4(+)IFN-gamma(+) T cells and soluble IFN-gamma compared to HUU newborns.
IMMUNITY INFLAMMATION AND DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Daiki Karigane, Miho Haraguchi, Noriko Toyama-Sorimachi, Emi K. Nishimura, Keiyo Takubo
Summary: Thymic dendritic cells (DCs) play a pivotal role in immune tolerance by regulating negative selection of autoreactive T cells in the thymus. This study reveals that the transcription factor Mitf is involved in the regulation of DC homing to the thymus and maintenance of thymic DC population.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Panayota Kolypetri, Howard L. Weiner
Summary: This review discusses the regulation of monocyte homeostasis and function by gut microbial signals and highlights the therapeutic potential of microbial signal-based approaches in various diseases.
TRENDS IN MICROBIOLOGY
(2023)
Article
Immunology
Seyed Mohammad Lellahi, Waqas Azeem, Yaping Hua, Benjamin Gabriel, Kristin Paulsen Rye, Hakon Reikvam, Karl-Henning Kalland
Summary: Conventional type 1 dendritic cells (cDC1) and conventional type 2 dendritic cells (cDC2) are being considered as alternative options to monocyte-derived dendritic cells (moDCs) in cancer immunotherapy. However, the low numbers of cDCs in peripheral blood and their extensive spontaneous apoptosis and death in culture within 24hrs pose challenges. The study found that GM-CSF improved the viability of both cDC1 and cDC2, while Flt3-L and IL-4 specifically increased the viability of cDC1 and cDC2, respectively. Combinations of these three cytokines further improved the viability of both cDCs at different time points.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Ganesh E. Phad, Pradeepa Pushparaj, Karen Tran, Viktoriya Dubrovskaya, Monika Adori, Paola Martinez-Murillo, Nestor Vazquez Bernat, Suruchi Singh, Gilman Dionne, Sijy O'Dell, Komal Bhullar, Sanjana Narang, Chiara Sorini, Eduardo J. Villablanca, Christopher Sundling, Benjamin Murrell, John R. Mascola, Lawrence Shapiro, Marie Pancera, Marcel Martin, Martin Corcoran, Richard T. Wyatt, Gunilla B. Karlsson Hedestam
JOURNAL OF EXPERIMENTAL MEDICINE
(2020)
Article
Clinical Neurology
Annamaria Finardi, Martina Diceglie, Luca Carbone, Caterina Arno, Alessandra Mandelli, Giuseppe De Santis, Maya Fedeli, Paolo Dellabona, Giulia Casorati, Roberto Furlan
FRONTIERS IN NEUROLOGY
(2020)
Editorial Material
Immunology
Georgia Fousteri, Ely Montee Rodrigues, Gian Maria Giamporcaro, Marika Falcone
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Immunology
Elza Evren, Emma Ringqvist, Kumar Parijat Tripathi, Natalie Sleiers, Ines Co Rives, Arlisa Alisjahbana, Yu Gao, Dhifaf Sarhan, Tor Halle, Chiara Sorini, Rico Lepzien, Nicole Marquardt, Jakob Michaelsson, Anna Smed-Sorensen, Johan Botling, Mikael C. Karlsson, Eduardo J. Villablanca, Tim Willinger
Summary: This study investigates the development of lung macrophages from human hematopoiesis using a humanized mouse model. The results show that different types of blood monocytes generate macrophages in distinct anatomical niches in the lung. Additionally, single-cell RNA sequencing reveals intermediate differentiation stages in human lung macrophage development.
Article
Multidisciplinary Sciences
Gloria Delfanti, Alessandra Perini, Elisa Zappa, Maya Fedeli
Summary: iNKT cells, a rare but important cell type in disease research, can be purified and expanded from mouse spleens using a specific method. The expanded iNKT cells can be easily used for in vitro and in vivo experiments, providing a valuable tool for studying activation and functional mechanisms.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Editorial Material
Immunology
Chiara Sorini, Eduardo J. Villablanca
Summary: In this issue of Immunity, Wang et al. demonstrate that the recognition of lysophosphatidyl serine via the receptor GPR43 allows type 3 innate lymphoid cells to sense damage-induced cell death, leading to interleukin-22-dependent tissue repair.
Article
Cell Biology
Oscar E. Diaz, Chiara Sorini, Rodrigo A. Morales, Xinxin Luo, Annika Frede, Annette M. Krais, Myra N. Chavez, Emma Wincent, Srustidhar Das, Eduardo J. Villablanca
Summary: The study revealed that PFOS exacerbates inflammation-induced intestinal damage, disrupts T-cell homeostasis, and provides a novel in vivo toolbox for screening pollutants affecting intestinal homeostasis.
DISEASE MODELS & MECHANISMS
(2021)
Article
Immunology
Claudia A. Rivera, Violaine Randrian, Wilfrid Richer, Yohan Gerber-Ferder, Maria-Graciela Delgado, Aleksandra S. Chikina, Annika Frede, Chiara Sorini, Mathieu Maurin, Hana Kammoun-Chaari, Sara M. Parigi, Christel Goudot, Mar Cabeza-Cabrerizo, Sylvain Baulande, Sonia Lameiras, Pierre Guermonprez, Caetano Reis e Sousa, Marc Lecuit, Helene D. Moreau, Julie Helft, Danijela Matic Vignjevic, Eduardo J. Villablanca, Ana-Maria Lennon-Dumenil
Summary: There are two pools of cDC2s in the small intestine, originating from common pre-DC precursors, and their phenotypes are influenced by food-derived retinoic acid.
Review
Medicine, General & Internal
Gloria Delfanti, Paolo Dellabona, Giulia Casorati, Maya Fedeli
Summary: Invariant Natural Killer T (iNKT) cells are a type of lymphocytes that can combat solid and hematological malignancies. They have unique functional features and are attractive for cell immunotherapy. iNKT cells can directly kill cancer cells and suppress immunosuppressive cells in the tumor microenvironment. Moreover, they can be transplanted across MHC barriers without causing alloreaction because of the identical CD1d molecules in all individuals.
FRONTIERS IN MEDICINE
(2022)
Article
Hematology
Chenyan Wu, Daryl Boey, Oscar Bril, Jennine Grootens, M. S. Vijayabaskar, Chiara Sorini, Maria Ekoff, Nicola K. Wilson, Johanna S. Ungerstedt, Gunnar Nilsson, Joakim S. Dahlin
Summary: Mast cell accumulation is a characteristic of various diseases, and this study utilized single-cell transcriptomics analysis to determine the timing of FcεRI appearance during mast cell progenitor stage. External stimuli were also shown to regulate FcεRI expression in mast cell progenitors.
Article
Biology
Cristina Faccani, Gianluca Rotta, Francesca Clemente, Maya Fedeli, Danilo Abbati, Francesco Manfredi, Alessia Potenza, Achille Anselmo, Federica Pedica, Guido Fiorentini, Chiara Villa, Maria P. Protti, Claudio Doglioni, Luca Aldrighetti, Chiara Bonini, Giulia Casorati, Paolo Dellabona, Claudia de Lalla
Summary: This study describes a workflow for high-dimensional flow cytometry analysis of T cells infiltrating liver metastatic tumor lesions. By optimizing the flow cytometer settings, establishing a multi-color antibody panel, and accurately tuning the dissociation of samples, the phenotypic complexity of T cells infiltrating the tumor lesions was effectively captured. This study provides a robust tool for analyzing complex T cell populations and can be adapted to characterize other relevant pathological tissues.
LIFE SCIENCE ALLIANCE
(2022)
Article
Immunology
Gloria Delfanti, Filippo Cortesi, Alessandra Perini, Gaia Antonini, Laura Azzimonti, Claudia de Lalla, Claudio Garavaglia, Mario L. Squadrito, Maya Fedeli, Michela Consonni, Silvia Sesana, Francesca Re, Haifa Shen, Paolo Dellabona, Giulia Casorati
Summary: Adoptive immunotherapy using T cells engineered with tumor-specific TCRs holds promise for cancer treatment. In this study, researchers harnessed iNKT cells with a second TCR specific for a tumor-associated peptide to generate bispecific effectors for CD1d- and MHC-restricted antigens. The TCR-engineered iNKT cells demonstrated high efficacy in restraining tumor progression and achieved robust cancer control by simultaneously modulating intratumoral suppressive myeloid populations and killing malignant cells. These findings support the potential use of tumor-redirected TCR-iNKT cells and local alpha-GalCer boosting as a therapy for cancer patients.
SCIENCE IMMUNOLOGY
(2022)
Article
Immunology
Annika Frede, Paulo Czarnewski, Gustavo Monasterio, Kumar P. Tripathi, David A. Bejarano, Ricardo O. Ramirez Flores, Chiara Sorini, Ludvig Larsson, Xinxin Luo, Laura Geerlings, Claudio Novella-Rausell, Chiara Zagami, Raoul Kuiper, Rodrigo A. Morales, Francisca Castillo, Matthew Hunt, Livia Lacerda Mariano, Yue O. O. Hu, Camilla Engblom, Ana-Maria Lennon-Dumenil, Romy Mittenzwei, Astrid M. Westendorf, Nadine Hoevelmeyer, Joakim Lundeberg, Julio Saez-Rodriguez, Andreas Schlitzer, Srustidhar Das, Eduardo J. Villablanca
Summary: In the process of intestinal damage and regeneration, B cells play a dominant role and show expansion of an IFN-induced B cell subset during mucosal healing. Depletion of B cells accelerates recovery, reduces epithelial ulceration, and enhances gene expression programs associated with tissue remodeling. The expansion of B cells impairs interactions between epithelial and stromal cells, hindering mucosal healing.
Article
Medicine, Research & Experimental
Chiara Sorini, Kumar P. Tripathi, Shengru Wu, Shawn M. Higdon, Jing Wang, Liqin Cheng, Sanghita Banerjee, Annika Reinhardt, Taras Kreslavsky, Anders Thorell, Lars Engstrand, Juan Du, Eduardo J. Villablanca
Summary: Helicobacter pylori colonization in asymptomatic individuals leads to significant changes in the composition of the gastric microbiome and immune cells. Metabolic and immune response pathways are altered. B cells are activated and progress into a highly proliferating stage, correlating with the presence of tertiary lymphoid structures. Our study provides a comprehensive atlas of the gastric mucosa-associated microbiome and immune cell landscape in asymptomatic H. pylori-infected individuals.
Article
Immunology
Maya Fedeli, Mirela Kuka, Annamaria Finardi, Francesca Albano, Valentina Vigano, Matteo Iannacone, Roberto Furlan, Paolo Dellabona, Giulia Casorati
Summary: miR-21 plays a crucial role in CD28-dependent costimulation pathways in T cells, decreasing the activation threshold and enhancing sensitivity to antigenic stimulation and survival, thereby broadening immune surveillance range. However, miR-21 may also lead to the unleashing of autoimmunity.
CLINICAL & TRANSLATIONAL IMMUNOLOGY
(2021)
