Article
Microbiology
Shiwen Peng, Deyin Xing, Louise Ferrall, Ya-Chea Tsai, Richard B. S. Roden, Chien-Fu Hung, T. -C. Wu
Summary: Our research focused on eliminating the confounding effects of dominant HPV antigen presentation through murine MHC-I via epitope mutagenesis, while ensuring oncogenicity. Mutated DNA constructs of HPV16 E6 and E7 were able to abolish murine MHC-I presentation and instead use human HLA-A2 molecules for presentation. These mutated constructs still maintained their oncogenic properties.
Article
Microbiology
Shiwen Peng, Deyin Xing, Louise Ferrall, Ya-Chea Tsai, Richard B. S. Roden, Chien-Fu Hung, T-C Wu
Summary: The study developed a spontaneous HPV16-driven HNSCC model in HLA-A2 transgenic mice to test candidate immunotherapies. They eliminated the confounding effects of dominant HPV antigen presentation through epitope mutagenesis and characterized their presentation in murine and human MHC-I molecules.
Article
Cell Biology
Shiwen Peng, Deyin Xing, Louise Ferrall, Ya-Chea Tsai, Chien-Fu Hung, T-C Wu
Summary: In this study, the authors characterized HPV18 E6/E7-specific CD8 + T cell epitopes in human HLA class I transgenic mice and identified a mutant HPV18E6 that retains its oncogenic properties, which has significant implications for vaccine development and treatment of HPV-related cancers.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Virology
Shuo Li, Mengxia Ye, Yonglin Chen, Quan Gong, Bing Mei
Summary: This study sheds light on the relationship between HPV-52 and cervical cancer, identifying variations in the E6-E7 genes and analyzing the protein's structure and selective pressure. Multiple single nucleotide variants were found in the E6 and E7 sequences of HPV-52, some impacting B cell epitopes.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Microbiology
James C. Romero-Masters, Laura K. Muehlbauer, Mitchell Hayes, Miranda Grace, Evgenia Shishkova, Joshua J. Coon, Karl Munger, Paul F. Lambert
Summary: The E6 protein encoded by MmuPV1 has potent oncogenic activities in mouse keratinocytes, including inhibition of differentiation, promotion of proliferation, and decreased dependence on growth factors. Proteomic and transcriptomic analyses revealed that MmuPV1 E6 upregulates the expression of proteins related to cellular proliferation and other cancer hallmarks. These findings indicate that MmuPV1 E6 is a major driver of MmuPV1-induced pathogenesis.
Article
Virology
Sadhana M. Gupta, Himangi Warke, Hemangi Chaudhari, Padmaja Mavani, Rajshree D. Katke, Shilpa C. Kerkar, Jayanti Mania-Pramanik
Summary: This study aimed to evaluate HPV E6/E7 oncogene expression as markers for early identification of cervical cancer risk in women with minor cytological abnormalities and negative cytology. The results showed that E6 and E7 transcripts could serve as sensitive early predictors of cervical cancer risk.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Review
Virology
James C. Romero-Masters, Paul F. Lambert, Karl Munger
Summary: HPVs cause a wide range of human diseases, from benign warts to malignant cancers. The discovery of a murine papillomavirus that infects laboratory mice provides an opportunity to study HPV-induced malignant diseases. The E6 and E7 proteins encoded by high-risk HPVs contribute to HPV-induced cancers in multiple ways.
Article
Virology
Mengxia Ye, Shuo Li, Ping Luo, Xuan Tang, Quan Gong, Bing Mei
Summary: This study analyzed the variations and phylogenetic relationship of HPV-51 virus in central China, as well as predicted its immune epitopes. The results showed high prevalence and genetic variations of HPV-51 in the region. These findings are of great importance for future research on vaccines with wider coverage and the development of diagnostic probes.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katsuyoshi Kumagai, Masakatsu Takanashi, Shin-ichiro Ohno, Yuichirou Harada, Koji Fujita, Keiki Oikawa, Katsuko Sudo, Shun-ichi Ikeda, Hirotaka Nishi, Kosuke Oikawa, Masahiko Kuroda
Summary: Cervical cancer development involves HPV infection and estrogen receptor signaling. WAPL plays a crucial role in CIN development by activating MACROD1 to increase ESR1 sensitivity, thereby promoting the expression of MYC and Cyclin D1.
Article
Medicine, Research & Experimental
Diane Bruyere, Patrick Roncarati, Alizee Lebeau, Thomas Lerho, Florian Poulain, Elodie Hendrick, Charlotte Pilard, Celia Reynders, Marie Ancion, Margaux Luyckx, Michael Renard, Yves Jacob, Jean-Claude Twizere, Raphael Peiffer, Olivier Peulen, Philippe Delvenne, Pascale Hubert, Alison McBride, Nicolas Gillet, Murielle Masson, Michael Herfs
Summary: HPV-positive cancers are usually associated with a favorable outcome due to high sensitivity to radiation therapy. However, the impact of viral E6/E7 oncoproteins on cellular radiosensitivity and host DNA repair is still largely speculative.
Article
Oncology
Paolo Giorgi Rossi, Guglielmo Ronco, Pamela Mancuso, Francesca Carozzi, Elena Allia, Simonetta Bisanzi, Anna Gillio-Tos, Laura De Marco, Raffaella Rizzolo, Daniela Gustinucci, Annarosa Del Mistro, Helena Frayle, Massimo Confortini, Anna Iossa, Elena Cesarini, Simonetta Bulletti, Basilio Passamonti, Silvia Gori, Laura Toniolo, Alessandra Barca, Laura Bonvicini, Francesco Venturelli, Maria Benevolo
Summary: As a primary screening test, E6/E7 mRNA has shown high sensitivity but a high positivity rate, requiring triage strategies. In this study, using mRNA as a primary test would miss about 3% of CIN3+ cases.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Microbiology
Xuelian Rao, Liangkai Zheng, Kaipeng Wei, Meiqing Li, Meng Jiang, Jianlong Qiu, Yulin Zhou, Rongqin Ke, Chen Lin
Summary: In situ detection of viral mRNA expression in tissue samples is valuable for pathological diagnosis. However, conventional RNA in situ hybridization assays lack sensitivity and specificity for clinical purposes. This study developed a novel in situ hybridization assay for HPV E6/E7 mRNA, which allows visualization of mRNA as discrete dot-like signals using bright-field microscopy. The results are consistent with traditional staining methods and offer potential applications for clinical diagnostics.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
Zi-Yi Yan, Xing-Hong Di, Yi Qiu, Yuan-Yuan Ying, Jun Gan, Hui-Hui Xu
Summary: This study investigates the genetic variations and carcinogenicity of HPV33 among women in Taizhou, Southeast China. The results show that the majority of HPV33 in this region belongs to the A1 subtype and there are no significant trends between nucleotide substitutions and cervical lesion risk. This study provides comprehensive data on HPV33 genetic variations and carcinogenicity in Southeast China.
Article
Oncology
Aribert Maryosly Castro-Amaya, Leonardo Fernandez-Avila, Carlos Alfredo Barron-Gallardo, Carlos Eliu Moreno-Rios, Sarah Naomi Guevara-Hernandez, Maria Teresa Magana-Torres, Clarisa Jazmin Pelayo-Aguirre, Luis Felipe Jave-Suarez, Adriana Aguilar-Lemarroy
Summary: This study reveals the transformative potential of Beta-2 Human papillomaviruses, especially HPV122, in cervical cancer samples from western Mexico. These viruses can modulate genes regulated by Alpha-HPVs and utilize alternative strategies to modulate diverse signaling pathways, potentially playing an important role in co-infections during carcinogenesis.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Integrative & Complementary Medicine
Limei Cao, Ping He, Jun Yang, Xin Long, Yanqiu Chen, Li Yan, Deping Zhou
Summary: This study assessed the predictive value of HPV E6/E7 mRNA detection in the outcome of cervical low-grade squamous intraepithelial lesion (LSIL). The results showed that regression or negativity of HPV E6/E7 mRNA was associated with the outcome of LSIL-related lesions, suggesting its potential as a predictive marker for LSIL.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Virology
Tatiana M. Garcia-Bates, Mariana L. Palma, Chengli Shen, Andrea Gambotto, Bernard J. C. Macatangay, Robert L. Ferris, Charles R. Rinaldo, Robbie B. Mailliard
JOURNAL OF VIROLOGY
(2019)
Article
Microbiology
Kai Qin, Sushma Boppana, Victor Y. Du, Jonathan M. Carlson, Ling Yue, Dario A. Dilernia, Eric Hunter, Robbie B. Mailliard, Simon A. Mallal, Anju Bansal, Paul A. Goepfert
Review
Virology
Jan Kristoff, Charles R. Rinaldo, Robbie B. Mailliard
Editorial Material
Virology
Robbie B. Mailliard
Review
Virology
Shivkumar Biradar, Michael T. Lotze, Robbie B. Mailliard
Article
Medicine, General & Internal
Tatiana M. Garcia-Bates, Mariana L. Palma, Renee R. Anderko, Denise C. Hsu, Jintanat Ananworanich, Bette T. Korber, Gaurav D. Gaiha, Nittaya Phanuphak, Rasmi Thomas, Sodsai Tovanabutra, Bruce D. Walker, John W. Mellors, Paolo A. Piazza, Eugene Kroon, Sharon A. Riddler, Nelson L. Michael, Charles R. Rinaldo, Robbie B. Mailliard
Summary: The study demonstrated the efficacy of using MDC1 to selectively elicit CTL responses against conserved and topologically important HIV-1 epitopes. Epigraph and Network antigens showed higher sequence conservation and stronger CTL responses compared to overlapping Gag peptides. Importantly, CTL responses against topologically constrained Gag epitopes were enhanced in Network pool-initiated cultures.
Article
Virology
Kai Qin, Sushma Boppana, Jonathan M. Carlson, Andrew Fiore-Gartland, Jacob Files, Jie Zeng, Jeffrey Edberg, Robbie B. Mailliard, Christina Ochsenbauer, Anju Bansal, Paul Goepfert
Summary: Adapted HIV epitopes can enhance viral trans-infection by promoting dendritic cell maturation. Vaccine recipients with high HLA-I-associated adaptation have a higher risk of HIV infection. These findings suggest the need to optimize HIV vaccine design considering HLA-I-associated adaptations to reduce infection risk.
JOURNAL OF VIROLOGY
(2021)
Article
Surgery
Lillian M. Tran, Guangzhi Cong, Mohammad H. Eslami, Robbie B. Mailliard, Ulka Sachdev-Ost
Summary: The study found that symptomatic HIV-infected individuals were more likely to have advanced vascular disease and early perioperative mortality during PAD-related procedures, while the presentation and mortality between asymptomatic HIV-infected individuals and HIV-uninfected individuals were similar. All individuals with PAD and HIV infection were more likely to undergo lower extremity amputations.
JOURNAL OF VASCULAR SURGERY
(2022)
Article
Immunology
Shivkumar Biradar, Yash Agarwal, Michael T. Lotze, Moses T. Bility, Robbie B. Mailliard
Summary: This study characterizes the interactions between gamma-delta T cells and HIV in a mouse model and explores the immunotherapeutic potential of V delta 2 T cells in controlling HIV replication. The findings reveal that HIV infection affects the proportion and ex vivo expansion of V delta 2 T cells in BLT humanized mice, and the adoptive transfer of activated V delta 2 T cells does not control HIV replication but exacerbates viremia.
FRONTIERS IN IMMUNOLOGY
(2022)
Meeting Abstract
Oncology
Pranav Murthy, Kelvin Li, Renee R. Anderko, Aatur D. Singhi, Annissa DeSilva, Asmita Chopra, Abdulraham Y. Hammad, Alessandro Paniccia, Alison Morris, Kenneth K. Lee, Robbie B. Mailliard, Michael T. Lotze, Barbara Methe, Amer H. Zureikat
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Meeting Abstract
Immunology
Shivkumar Biradar, Michael Lotze, Robbie B. Mailliard
JOURNAL OF IMMUNOLOGY
(2020)
Meeting Abstract
Immunology
Renee R. Anderko, Charles R. Rinaldo, Robbie B. Mailliard
JOURNAL OF IMMUNOLOGY
(2020)
Article
Immunology
Renee R. Anderko, Charles R. Rinaldo, Robbie B. Mailliard
JOURNAL OF IMMUNOLOGY
(2020)
Meeting Abstract
Immunology
Renee R. Anderko, Charles R. Rinaldo, Robbie B. Mailliard
JOURNAL OF IMMUNOLOGY
(2019)