Review
Oncology
Nicole J. Toney, Jeffrey Schlom, Renee N. Donahue
Summary: Tumor biopsy is often difficult to obtain in patients with solid tumors. Investigation of the peripheral immune system allows for in-depth analysis of patient immune response during treatment. Phosphoflow cytometry is a method to detect phosphorylated proteins in single cells and can be used to determine signaling pathway responsiveness in specific immune subsets. This review summarizes the application of phosphoflow cytometry in cancer immunology, including the study of immune cells, tumor microenvironment, drug effects, and prediction of treatment response and outcome. It demonstrates the potential of phosphoflow cytometry in exploring mechanisms of immunotherapy response or resistance and improving cancer patient outcome.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Medicine, General & Internal
Pengqiang Zhong, Mengzhi Hong, Huanyu He, Jiang Zhang, Yaoming Chen, Zhigang Wang, Peisong Chen, Juan Ouyang
Summary: An AI model was developed to diagnose acute leukemia using MFC, showing shorter analysis time and higher consistency compared to manual methods.
Article
Biochemistry & Molecular Biology
Valentina Serra, Edoardo Fiorillo, Francesco Cucca, Valeria Orru
Summary: The use of cryopreserved peripheral blood mononuclear cells is common in biological research. We evaluated the viability, proliferation and cytokine production capabilities, as well as the levels of cell subtypes in frozen human peripheral blood mononuclear cells. We observed a progressive increase in cell death percentages on thawing, but the frequency of the main lymphocyte subsets was stable.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Kota Sato, Kiyoshi Okazuka, Tadao Ishida, Jun Sakamoto, Shigeto Kaneko, Junichiro Nashimoto, Yui Uto, Mizuki Ogura, Yumiko Yoshiki, Yu Abe, Aki Maeda, Hiroyuki Hamazaki, Nobuhiro Tsukada, Yuji Hiragohri, Kenshi Suzuki
Summary: MRD-negative status in multiple myeloma is associated with favorable outcomes, and EuroFlow next-generation flow is a global standard for MRD detection. However, its high operating cost necessitates the development of a less expensive method with equivalent sensitivity. In this study, our 10-color multiparameter flow cytometry demonstrated high sensitivity to detect MRD in MM patients, offering a promising, cost-effective alternative to EuroFlow-NGF.
ANNALS OF HEMATOLOGY
(2021)
Article
Immunology
Teresa Preglej, Marie Brinkmann, Guenter Steiner, Daniel Aletaha, Lisa Goeschl, Michael Bonelli
Summary: This study explores the potential of multi-parameter spectral flow cytometry as a diagnostic and evaluation tool for autoimmune diseases. It demonstrates the use of advanced immunophenotyping to profile cell subset distribution and cell activation, and suggests its application in drug screening and personalized treatment approaches.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Food Science & Technology
Katarzyna Sielatycka, Wojciech Juzwa, Joanna Sliwa-Dominiak, Mariusz Kaczmarczyk, Igor Loniewski, Wojciech Marlicz
Summary: Flow cytometry was used to assess the viability and number of freeze-dried bacteria in commercially available capsules, revealing that the total amount of viable bacteria in probiotic products varied between 70% and 90%, with dead cells ranging from 10% to 30%. FCM allowed for faster results compared to the traditional plate count method, with no significant differences found between the two protocols in terms of AFU/g obtained.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2021)
Article
Immunology
Olivia Lie-Andersen, Mie Linder Hubbe, Krishanthi Subramaniam, Daniel Steen-Jensen, Ann Christina Bergmann, Daniel Justesen, Morten Orebo Holmstrom, Lance Turtle, Sune Justesen, Telma Lanca, Morten Hansen
Summary: Recognition of antigenic epitopes relies on the presentation of epitopes to T cells through the HLA complex. This study demonstrates that considering the stability of the peptide:HLA complex in epitope selection significantly reduces false discovery rate. By measuring the stability of pHLA complex on three class I alleles and 1286 peptides derived from SARS-CoV-2 Spike protein, stable pHLA complexes were shown to select immunogenic epitopes that activate CD8(+)T cells. This finding is consistent across different alleles and in both vaccinated and convalescent COVID-19 donors. Evaluation of peptide pools reveals the recognition of dominant epitopes by specific CD8(+)T cells, and SARS-CoV-2 specific CD8(+)T cells were detected across multiple donors using tetramer-staining. In conclusion, stability analysis of pHLA is crucial for identifying immunogenic epitopes.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Melisa Gualdron-Lopez, Miriam Diaz-Varela, Haruka Toda, Iris Aparici-Herraiz, Laura Pedro-Cos, Ricardo Lauzurica, Marcus V. G. Lacerda, Marco Antonio Fernandez-Sanmartin, Carmen Fernandez-Becerra, Hernando A. del Portillo
Summary: The study provides a detailed characterization of human spleen cell populations, showing interactions with plasma-derived extracellular vesicles from Plasmodium vivax infected patients. The findings suggest an increased proportion of certain immune cells and erythrocytes interacting with PvEVs compared to EVs from healthy volunteers, indicating potential pathophysiological processes involving the spleen in vivax malaria.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Cell Biology
Tobias Maetzig, Anna Lieske, Nicole Doerpmund, Michael Rothe, Marc-Jens Kleppa, Violetta Dziadek, Jacob Jalil Hassan, Julia Dahlke, Dorit Borchert, Axel Schambach
Summary: This study developed a complex fluorescent genetic barcoding system for the tracking and analysis of AML clones. It revealed the competitive dynamics and evolution of AML clones and verified the underlying driver mechanisms behind clonal selection.
Article
Cell Biology
Omar Perbellini, Chiara Cavallini, Roberto Chignola, Marilisa Galasso, Maria T. Scupoli
Summary: This study reveals a high variability in signaling networks across different T-ALL cell lines and provides insight into the characterization of these differences using phospho-specific flow cytometry.
Article
Biochemistry & Molecular Biology
Manuel Fernandez-Delgado, Luis Sendra, Maria Jose Herrero, Gladys G. Olivera-Pasquini, Alexander Batista-Duharte, Salvador F. Alino
Summary: This study evaluated the ability of fluorescent scramble oligonucleotides (ON*) to access human peripheral blood mononuclear cells (PBMC) using flow cytometry. The results showed that ON* could rapidly access neutrophils and monocytes, reaching their maximum levels in 1 hour and 24 hours, respectively, while lymphocytes required 7 days to reach maximum transfection. Additionally, different lymphocyte types and T cell subtypes had similar accessibility to ON*, with T cells being more accessible.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Julia Kudlacova, Daniela Kuzilkova, Frantisek Barta, Nadezda Brdickova, Adela Vavrova, Libor Kostka, Ondrej Hovorka, Tomasc Kalina, Tomasc Etrych
Summary: This article describes an advanced bioconjugation technique used to synthesize hybrid polymer-antibody nanoprobes for fluorescent cell barcoding in flow cytometry-based immunophenotyping of leukocytes. The technique employs a novel approach of attachment combining two fluorescent dyes on the copolymer precursor and its conjugation to antibody, allowing the synthesis of barcoded nanoprobes of antibody polymer dyes. This technique is valuable for cellular assays that require low intersample variability.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Biochemical Research Methods
Alexander Heubeck, Adam Savage, Katherine Henderson, Charles Roll, Veronica Hernandez, Troy Torgerson, Thomas Bumol, Julian Reading
Summary: This article highlights the importance of immunophenotyping using high dimensional flow cytometry and presents four high parameter flow cytometry panels for deep immunophenotyping of human PBMC. These panels include 64 cell surface markers and provide insights into immune compartment populations. The panels were developed using two different cytometers and produced comparable high quality flow cytometry data, demonstrating their suitability for immune profiling experiments.
Article
Obstetrics & Gynecology
Stephania Vazquez Rodriguez, Lourdes A. Arriaga Pizano, Estibalitz Laresgoiti Servitje, Javier Mancilla Ramirez, Omar L. Peralta Mendez, Gisela Villalobos Alcazar, Martha L. Granados Cepeda, Maria Graciela Hernandez Pelaez, Guadalupe Cordero Gonzalez, Roberto Arizmendi Villanueva, Jose Luis Cruz Ramirez, Armando Isibasi, Constantino Lopez Macias, Leopoldo Flores Romo, Luis A. Jimenez Zamudiog, Arturo Cerbulo-Vazquez
Summary: The study reveals that multiple markers, such as CD45RA, CD45RO, CD71, HLA-DR, and TREM-1, may be useful for diagnosing neonatal sepsis. However, only the expression of CD45RO on T lymphocytes is identified as a useful biomarker for diagnosing neonatal late-onset sepsis. Additionally, decision tree analyses suggest that CD45RO combined with CD27 could help differentiate preterm septic neonates from those with risk factors.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2021)
Article
Hematology
Matthew S. Hindle, Lih T. Cheah, Daisie M. Yates, Khalid M. Naseem
Summary: This study aimed to characterize the effects of various preanalytical variables on blood platelet analysis using multiparameter fluorescent flow cytometry. Suboptimal conditions led to increased basal platelet activity and reduced sensitivity to stimulation, while optimal conditions protected platelets from artifactual stimulation and preserved basal activity and sensitivity to activation.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Immunology
R. Davies, D. Hammenfors, B. Bergum, K. Jakobsen, M. Solheim, P. Vogelsang, J. G. Brun, Y. Bryceson, R. Jonsson, S. Appel
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2017)
Article
Immunology
M. Karlsen, K. Jakobsen, R. Jonsson, D. Hammenfors, T. Hansen, S. Appel
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2017)
Article
Endocrinology & Metabolism
Jorge I. Ramirez Sepulveda, Marika Kvarnstrom, Per Eriksson, Thomas Mandl, Katrine Braekke Norheim, Svein Joar Johnsen, Daniel Hammenfors, Malin V. Jonsson, Kathrine Skarstein, Johan G. Brun, Lars Ronnblom, Helena Forsblad-d'Elia, Sara Magnusson Bucher, Eva Baecklund, Elke Theander, Roald Omdal, Roland Jonsson, Gunnel Nordmark, Marie Wahren-Herlenius
BIOLOGY OF SEX DIFFERENCES
(2017)
Article
Dermatology
A. K. Aarebrot, S. M. Solberg, R. Davies, L. I. Bader, T. D. Holmes, S. Gavasso, Y. T. Bryceson, R. Jonsson, L. F. Sandvik, S. Appel
BRITISH JOURNAL OF DERMATOLOGY
(2018)
Article
Immunology
Richard Davies, Daniel Hammenfors, Brith Bergum, Petra Vogelsang, Sonia Gavasso, Johan G. Brun, Roland Jonsson, Silke Appel
EUROPEAN JOURNAL OF IMMUNOLOGY
(2018)
Article
Immunology
Anke Fuchs, Mateusz Gliwinski, Nathali Grageda, Rachel Spiering, Abul K. Abbas, Silke Appel, Rosa Bacchetta, Manuela Battaglia, David Berglund, Bruce Blazar, Jeffrey A. Bluestone, Martin Bornhaeuser, Anja ten Brinke, Todd M. Brusko, Nathalie Cools, Maria Cristina Cuturi, Edward Geissler, Nick Giannoukakis, Karolina Golab, David A. Hafler, S. Marieke van Ham, Joanna Hester, Keli Hippen, Mauro Di Ianni, Natasa Ilic, John Isaacs, Fadi Issa, Dorota Iwaszkiewicz-Grzes, Elmar Jaeckel, Irma Joosten, David Klatzmann, Hans Koenen, Cees van Kooten, Olle Korsgren, Karsten Kretschmer, Megan Levings, Natalia Maria Marek-Trzonkowska, Marc Martinez-Llordella, Djordje Miljkovic, Kingston H. G. Mills, Joana P. Miranda, Ciriaco A. Piccirillo, Amy L. Putnam, Thomas Ritter, Maria Grazia Roncarolo, Shimon Sakaguchi, Silvia Sanchez-Ramon, Birgit Sawitzki, Ljiljana Sofronic-Milosavljevic, Megan Sykes, Qizhi Tang, Marta Vives-Pi, Herman Waldmann, Piotr Witkowski, Kathryn J. Wood, Silvia Gregori, Catharien M. U. Hilkens, Giovanna Lombardi, Phillip Lord, Eva M. Martinez-Caceres, Piotr Trzonkowski
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Oncology
Dag Heiro Yi, Nadine Stetter, Kjerstin Jakobsen, Roland Jonsson, Silke Appel
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2018)
Article
Immunology
Silje M. Solberg, Lene F. Sandvik, Marianne Eidsheim, Roland Jonsson, Yenan T. Bryceson, Silke Appel
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2018)
Article
Immunology
Alexander Sauter, Dag Heiro Yi, Yayan Li, Sabine Roersma, Silke Appel
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Immunology
Waqas Azeem, Ragnhild Maukon Bakke, Silke Appel, Anne Margrete Oyan, Karl-Henning Kalland
FRONTIERS IN IMMUNOLOGY
(2020)
Review
Immunology
Lisa Lynn ten Bergen, Aleksandra Petrovic, Anders Krogh Aarebrot, Silke Appel
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2020)
Review
Immunology
Lisa Lynn ten Bergen, Aleksandra Petrovic, Anders Krogh Aarebrot, Silke Appel
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2020)
Article
Immunology
Silje Michelsen Solberg, Anders Krogh Aarebrot, Irene Sarkar, Aleksandra Petrovic, Lene Froyen Sandvik, Brith Bergum, Roland Jonsson, Yenan Troy Bryceson, Silke Appel
Summary: This study analyzed peripheral blood mononuclear cells (PBMCs) in psoriasis patients and healthy controls, finding an increase in certain immune cells in severe psoriasis patients, and relief of systemic inflammation after biological treatment.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Immunology
Richard Davies, Irene Sarkar, Daniel Hammenfors, Brith Bergum, Petra Vogelsang, Silje M. Solberg, Sonia Gavasso, Johan G. Brun, Roland Jonsson, Silke Appel
FRONTIERS IN IMMUNOLOGY
(2019)