Journal
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES
Volume 36, Issue 1, Pages 41-47Publisher
SPRINGER
DOI: 10.1007/s11596-016-1539-1
Keywords
renal fibrosis; unilateral ureteral obstruction; PPAR-gamma; T lymphocyte
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Funding
- National Natural Science Foundation of China [81470948, 81270770, 81300575]
- Hubei Provincial Health and Family Planning Youth Project of China [WJ2015Q007]
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Renal tubulointerstitial fibrosis is the common ending of progre beta sive renal disease. It is worth developing new ways to stop the progre beta s of renal fibrosis. Peroxisome proliferator-activated receptor-gamma (PPAR gamma) agonists have been studied to treat diabetic nephropathy, cisplatin-induced acute renal injury, ischemia reperfusion injury and adriamycin nephropathy. In this study, unilateral ureteral obstruction (UUO) was used to establish a different renal fibrosis model. PPAR? agonist pioglitazone was administrated by oral gavage and saline was used as control. At 7th and 14th day after the operation, mice were sacrificed for fibrosis test and T lymphocytes subsets test. Unexpectedly, through MASSON staining, immunohistochemistry for alpha-SMA, and Western blotting for a-SMA and PDGFR-beta, we found that pioglitazone failed to attenuate renal fibrosis in UUO mice. However, flow cytometry showed that pioglitazone down-regulated Th1 cells, and up-regulated Th2 cells, Th17 cells and Treg cells. But the Th17/Treg ratio had no significant change by pioglitazone. Real-time PCR results showed that TGF-beta and MCP-1 had no significant changes, at the same time, CD4(+) T cells associated cytokines were partially regulated by pioglitazone pretreatment. Taken together, pioglitazone failed to suppress renal fibrosis progression caused by UUO.
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