Journal
JOURNAL OF HOSPITAL INFECTION
Volume 93, Issue 2, Pages 206-210Publisher
W B SAUNDERS CO LTD
DOI: 10.1016/j.jhin.2016.02.020
Keywords
Enterobacteriaceae; Blood culture bottles; MALDI-TOF MS; Third-generation cephalosporin resistance; Cefotaxime
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An ease-of-use protocol for the identification of resistance against third-generation cephalosporins in Enterobacteriaceae isolated from blood culture bottles was evaluated using matrix-assisted laser desorption ionizationetime-of-flight mass spectrometry. A cefotaxime hydrolysis assay from chocolate agar subcultures using antibiotic discs and without inoculum standardization was developed for routine work flow, with minimal hands-on time. This assay showed good performance in distinguishing between cefotaxime-susceptible and cefotaxime-resistant strains, with excellent results for Escherichia coli (sensitivity 94.7%, specificity 100%). However, cefotaxime resistance was not detected reliably in Enterobacteriaceae expressing AmpC genes or carbapenemase-producing Klebsiella pneumoniae. (C) 2016 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.
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