Article
Medicine, General & Internal
Sodai Sakamoto, Hiroki Bochimoto, Kengo Shibata, Nur Khatijah Mohd Zin, Moto Fukai, Kosei Nakamura, Takahisa Ishikawa, Masato Fujiyoshi, Tsuyoshi Shimamura, Akinobu Taketomi
Summary: This study aimed to verify the optimal pH of hypothermic machine perfusion (HMP) for donation after circulatory death (DCD) livers. The results showed that the HMP group with pH 7.8 displayed significant graft protection, as evidenced by lower enzyme levels, bile production, diminished tissue injury, and well-preserved mitochondrial structure. Therefore, HMP with a pH of 7.8 enhances protective effects by preserving mitochondrial structure and function, reducing reperfusion injury in DCD livers.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
R. Van Rijn, I. J. Schurink, Y. de Vries, A. P. van den Berg, M. Cortes Cerisuelo, S. Darwish Murad, J. I. Erdmann, N. Gilbo, R. J. de Haas, N. Heaton, B. van Hoek, V. A. L. Huurman, I. Jochmans, O. B. van Leeuwen, V. E. de Meijer, D. Monbaliu, W. G. Polak, J. J. G. Slangen, R. I. Troisi, A. Vanlander, J. de Jonge, R. J. Porte
Summary: This study demonstrated that hypothermic oxygenated machine perfusion can reduce the risk of nonanastomotic biliary strictures following liver transplantation from donors after circulatory death, with lower rates of complications compared to conventional static cold storage.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Gastroenterology & Hepatology
Guillaume Rossignol, Xavier Muller, Valerie Hervieu, Sophie Collardeau-Frachon, Antoine Breton, Natacha Boulanger, Mickael Lesurtel, Remi Dubois, Kayvan Mohkam, Jean-Yves Mabrut
Summary: This single-center pilot study presents feasibility and safety data for transplantation of partial liver grafts undergoing ex situ split during HOPE, suggesting improved preservation compared with static ex situ splitting.
LIVER TRANSPLANTATION
(2022)
Article
Gastroenterology & Hepatology
Andrea Schlegel, Matteo Mueller, Xavier Mueller, Janina Eden, Rebecca Panconesi, Stefanie von Felten, Klaus Steigmiller, Richard X. Sousa Da Silva, Olivier de Rougemont, Jean-Yves Mabrut, Mickael Lesurtel, Miriam Cortes Cerisuelo, Nigel D. Heaton, Marc Antoine Allard, Rene Adam, Diethard Monbaliu, Ina Jochmans, Martijn P. D. Haring, Robert J. Porte, Alessandro Parente, Paolo Muiesan, Philipp Kron, Magdy Attia, Dagmar Kollmann, Gabriela Berlakovich, Xavier Rogiers, Karin Petterson, Anne L. Kranich, Stefanie Amberg, Beat Mullhaupt, Pierre-Alain Clavien, Philipp Dutkowski
Summary: Hypothermic oxygenated perfusion (HOPE) after cold storage of donated livers reduces the incidence of complications and graft failure in liver transplantation.
JOURNAL OF HEPATOLOGY
(2023)
Article
Cell Biology
Wei Zhou, Zibiao Zhong, Danni Lin, Zhongzhong Liu, Qiuyan Zhang, Haoyang Xia, Sheng Peng, Anxiong Liu, Zhongshan Lu, Yanfeng Wang, Shaojun Ye, Qifa Ye
Summary: Ischemia-reperfusion injury (IRI) is a serious issue in donations after heart death (DCD) liver transplantation, and hypothermic oxygenated perfusion (HOPE) has been shown to reduce liver IRI by regulating inflammation. HOPE can protect against DCD liver transplantation-induced-liver IRI by reducing inflammation via HECTD3-mediated TRAF3 K63-linked polyubiquitination.
CELL DEATH & DISEASE
(2021)
Article
Pathology
Francesco Vasuri, Mattia Riefolo, Matteo Ravaioli, Matteo Cescon, Gianandrea Pasquinelli, Giuliana Germinario, Antonia D'Errico
Summary: This study aimed to validate the impact of graft histology on transplant outcome and found that grafts with portal fibrosis stage = 3 had a higher incidence of early allograft dysfunction and 6-month dysfunction. Portal inflammation and lobular fibrosis were also correlated with post-transplant kidney function and graft survival. However, the use of HOPE significantly reduced the risk factor of portal inflammation on graft survival.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Medicine, General & Internal
Rebecca Panconesi, Mauricio Flores Carvalho, Janina Eden, Marilena Fazi, Fariha Ansari, Leandro Mancina, Nadia Navari, Richard Xavier Sousa Da Silva, Daniele Dondossola, Lucia Bautista Borrego, Matthias Pietzke, Adriano Peris, David Meierhofer, Paolo Muiesan, Alexander Galkin, Fabio Marra, Philipp Dutkowski, Andrea Schlegel
Summary: This study investigated the effects of NRP and HOPE on DCD liver transplantation using an animal model. The results showed that HOPE can reduce mitochondrial injury and inflammation, and improve post-transplant survival.
Article
Multidisciplinary Sciences
Jianbo Li, XiangJun Zha, Yan Kang, Zhongwei Zhang, Lvnan Yan, Lujia Song, Chengdi Wang, Jiayin Yang
Summary: A proposed oxygen-carrying sequential preservation (OCSP) method combining oxygenated static cold storage (SCS) and normothermic mechanical perfusion was found to decrease ischemia-reperfusion injury (IRI) and improve rat survival after transplantation. Oxygen-dependent autophagy activation was shown to mitigate liver graft's IRI by suppressing endoplasmic reticulum stress response (ERSR), and the NFE2L2-HMOX1 signaling pathway was found to induce autophagy under OCSP.
Review
Medicine, General & Internal
Damiano Patrono, Nicola De Stefano, Elena Vissio, Ana Lavinia Apostu, Nicoletta Petronio, Giovanni Vitelli, Giorgia Catalano, Giorgia Rizza, Silvia Catalano, Fabio Colli, Luigi Chiusa, Renato Romagnoli
Summary: Liver allograft steatosis poses a significant risk to transplant outcomes and survival. With the increasing prevalence of obesity and fatty liver disease, the use of steatotic liver grafts in transplantation has become more common. This review examines the mechanisms behind fatty liver susceptibility to ischemia-reperfusion injury and explores strategies, including donor interventions, preservation solutions, and machine perfusion techniques, to enhance the utilization of steatotic livers for transplantation.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Medicine, General & Internal
Joseph Mugaanyi, Lei Dai, Changjiang Lu, Shuqi Mao, Jing Huang, Caide Lu
Summary: Machine perfusion, particularly HOPE/D-HOPE and NMP, shows promise as alternatives to static cold storage for the preservation of donor livers. These methods improve graft and patient survival rates and reduce the incidence of biliary complications. They have the potential to address the gap between demand and availability of donor livers by enabling the utilization of marginal livers.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Fabian Horne, Moritz Drefs, Malte Joachim Schirren, Dominik Thomas Koch, Ganildo Cepele, Severin Johannes Jacobi, Elnaz Payani, Nikolaus Boerner, Jens Werner, Markus Otto Guba, Dionysios Koliogiannis
Summary: The study demonstrates that pre-transplant graft preconditioning with HOPE can reduce the incidence of PRS and EAD in liver transplantation, as well as improve hemodynamic stability. HOPE can also mitigate ES and prevent hyperpotassemic complications.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Medicine, General & Internal
Fabio Melandro, Riccardo De Carlis, Francesco Torri, Andrea Lauterio, Paolo De Simone, Luciano De Carlis, Davide Ghinolfi
Summary: This review summarizes the current literature on viability criteria during normothermic and hypothermic machine perfusion, and discusses the future steps and evolution of these technologies.
MEDICINA-LITHUANIA
(2022)
Article
Surgery
Qiang Liu, Luca Del Prete, Khaled Ali, Patrick Grady, Mary Bilancini, John Etterling, Giuseppe D'Amico, Teresa Diago Uso, Koji Hashimoto, Federico Aucejo, Masato Fujiki, Bijan Eghtesad, Kazunari Sasaki, Choon Hyuck David Kwon, Sulemon Chaudhry, Junshi Doi, Alejandro Pita, Brandon New, Ana Bennett, Jacek Cywinski, Charles Miller, Cristiano Quintini
Summary: The purpose of this study was to assess the safety and feasibility of sequential hypothermic oxygenated perfusion and normothermic machine perfusion and the potential benefits of graft viability preservation and assessment before liver transplantation. 17 expanded criteria donor livers underwent sequential hypothermic oxygenated perfusion and normothermic machine perfusion. The results showed that sequential hypothermic oxygenated perfusion and normothermic machine perfusion preservation is safe and feasible and has the potential benefits of preserving and evaluating expanded criteria donor livers.
Article
Engineering, Biomedical
Fan Lin, Fu Zhen, Xiong Yan, Ye Shaojun, Peng Guizhu, Wang Yanfeng, Ye Qifa
Summary: The study found that HOPE can protect steatotic liver grafts and improve liver function, with the addition of DF enhancing the efficacy of HOPE. While DF cannot promote lipid decomposition of steatotic liver grafts, it can improve steatotic liver function and postoperative survival compared to HOPE.
Article
Health Care Sciences & Services
Federica Rigo, Nicola De Stefano, Damiano Patrono, Victor De Donato, Ludovico Campi, Diana Turturica, Teresa Doria, Veronica Sciannameo, Paola Berchialla, Francesco Tandoi, Renato Romagnoli
Summary: The study aimed to investigate the impact of dual-hypothermic oxygenated machine perfusion (D-HOPE) on hepatocellular carcinoma (HCC) recurrence after liver transplantation (LT). The results showed that D-HOPE did not reduce HCC recurrence but allowed the use of extended criteria donor livers, with comparable postoperative outcomes to static cold storage (SCS).
JOURNAL OF PERSONALIZED MEDICINE
(2023)
