A cleavable self-aggregating tag scheme for the expression and purification of disulfide bonded proteins and peptides

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(2021) Lisa Urquhart   NATURE REVIEWS DRUG DISCOVERY

Development of Protein-Specific Analytical Methodologies to Evaluate Compatibility of Recombinant Human (rh)IGF-1/rhIGFBP-3 with Intravenous Medications Co-Administered to Neonates

(2021) Nazila Salamat-Miller et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Facile expression and purification of active human growth hormone in E. coli by a cleavable self-aggregating tag scheme

(2021) Zhanglin Lin et al.   PROTEIN EXPRESSION AND PURIFICATION

Over‐expression, overproduction, purification and characterization of rhGH in E. coli

(2020) Reyhane Keshavarz et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

An expanded library of orthogonal split inteins enables modular multi-peptide assemblies

(2020) Filipe Pinto et al.   Nature Communications

A linker of the proline-threonine repeating motif sequence is bimodal

(2020) Munir Salomão Skaf et al.   JOURNAL OF MOLECULAR MODELING

New tools for recombinant protein production in Escherichia coli : A 5-year update

(2019) Germán L. Rosano et al.   PROTEIN SCIENCE

Escherichia coli “TatExpress” strains export several g/L human growth hormone to the periplasm by the Tat pathway

(2019) Isabel Guerrero Montero et al.   BIOTECHNOLOGY AND BIOENGINEERING

Engineered pH‐inducible intein Mtu ΔI‐CM variants with markedly reduced premature cleavage activity

(2019) Zhanglin Lin et al.   AICHE JOURNAL

Strategies for optimization of heterologous protein expression in E. coli : Roadblocks and reinforcements

(2018) Jashandeep Kaur et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Determination of recombinant Interferon-α2 in E. coli periplasmic extracts by reversed-phase high-performance liquid chromatography

(2018) Paulo V.S. Dias et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

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(2018) Gary Walsh   NATURE BIOTECHNOLOGY

Improved production of single domain antibodies with two disulfide bonds by co-expression of chaperone proteins in the Escherichia coli periplasm

(2017) Lisa C. Shriver-Lake et al.   JOURNAL OF IMMUNOLOGICAL METHODS

A cleavable self-assembling tag strategy for preparing proteins and peptides with an authentic N-terminus

(2017) Qing Zhao et al.   Biotechnology Journal

Recombinant production of medium- to large-sized peptides in Escherichia coli using a cleavable self-aggregating tag

(2016) Qing Zhao et al.   Microbial Cell Factories

High purity recombinant human growth hormone (rhGH) expression in Escherichia coli under phoA promoter

(2016) Hao Song et al.   Bioengineered

Challenges and recent advances in affinity purification of tag-free proteins

(2014) Dongli Guan et al.   BIOTECHNOLOGY LETTERS

Soluble Expression of Disulfide Bond Containing Proteins FGF15 and FGF19 in the Cytoplasm of Escherichia coli

(2014) Bo Kong et al.   PLoS One

Facile expression and purification of the antimicrobial peptide histatin 1 with a cleavable self-aggregating tag (cSAT) in Escherichia coli

(2013) Lei Xing et al.   PROTEIN EXPRESSION AND PURIFICATION

Protein refolding using chemical refolding additives

(2012) Satoshi Yamaguchi et al.   Biotechnology Journal

SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

(2012) Julie Lobstein et al.   Microbial Cell Factories

Small surfactant-like peptides can drive soluble proteins into active aggregates

(2012) Bihong Zhou et al.   Microbial Cell Factories

Linkers in the structural biology of protein-protein interactions

(2012) Vishnu Priyanka Reddy Chichili et al.   PROTEIN SCIENCE

Electronic Structure of Neighboring Extein Residue Modulates Intein C-Terminal Cleavage Activity

(2011) Philip T. Shemella et al.   BIOPHYSICAL JOURNAL

Streamlined protein expression and purification using cleavable self-aggregating tags

(2011) Lei Xing et al.   Microbial Cell Factories

Fabrication of self-assembling d-form peptide nanofiber scaffold d-EAK16 for rapid hemostasis

(2010) Zhongli Luo et al.   BIOMATERIALS

Production of recombinant proteins by microbes and higher organisms

(2009) Arnold L. Demain et al.   BIOTECHNOLOGY ADVANCES

Validation of an RP-LC Method for the Determination of Interferon-α2a in Pharmaceutical Formulations

(2009) Lucélia Magalhães da Silva et al.   JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Modulation of intein activity by its neighboring extein substrates

(2009) G. Amitai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA