An RT-RPA-Cas12a platform for rapid and sensitive detection of tilapia lake virus

Concentration and quantification of Tilapia tilapinevirus from water using a simple iron flocculation coupled with probe-based RT-qPCR

(2022) Suwimon Taengphu et al.   PeerJ

Figure of Merit for CRISPR-Based Nucleic Acid-Sensing Systems: Improvement Strategies and Performance Comparison

(2022) Reza Nouri et al.   ACS Sensors

Machine Learning-Driven and Smartphone-Based Fluorescence Detection for CRISPR Diagnostic of SARS-CoV-2

(2021) Aubin Samacoits et al.   ACS Omega

Integrating Reverse Transcription Recombinase Polymerase Amplification with CRISPR Technology for the One-Tube Assay of RNA

(2021) Wei Feng et al.   ANALYTICAL CHEMISTRY

Prevention and Control of Streptococcosis in Tilapia Culture: A Systematic Review

(2021) Sahya Maulu et al.   JOURNAL OF AQUATIC ANIMAL HEALTH

Development and comparative evaluation of real-time PCR and real-time RPA assays for detection of tilapia lake virus

(2021) Yahui Wang et al.   MOLECULAR AND CELLULAR PROBES

Good guide, bad guide: spacer sequence-dependent cleavage efficiency of Cas12a

(2020) Sjoerd C A Creutzburg et al.   NUCLEIC ACIDS RESEARCH

Genetic diversity of tilapia lake virus genome segment 1 from 2011 to 2019 and a newly validated semi-nested RT-PCR method

(2020) Suwimon Taengphu et al.   AQUACULTURE

Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection

(2020) Long T. Nguyen et al.   Nature Communications

Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

(2020) Maturada Patchsung et al.   Nature Biomedical Engineering

Viral infections in tilapines: More than just tilapia lake virus

(2019) Vimbai Irene Machimbirike et al.   AQUACULTURE

Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing

(2019) Benjamin P. Kleinstiver et al.   NATURE BIOTECHNOLOGY

Development of a simple and rapid reverse transcription–loopmediated isothermal amplification (RT‐LAMP) assay for sensitive detection of tilapia lake virus

(2019) Jiyuan Yin et al.   JOURNAL OF FISH DISEASES

Rapid detection of tilapia lake virus using a one-step reverse transcription loop-mediated isothermal amplification assay

(2019) Theerawut Phusantisampan et al.   AQUACULTURE

Potential application of CRISPR-Cas12a fluorescence assay coupled with rapid nucleic acid amplification for detection of white spot syndrome virus in shrimp

(2019) Thawatchai Chaijarasphong et al.   AQUACULTURE

Hematological analysis and relative condition factor in naturally parasitized Nile tilapia

(2019) Lilian Dordete Steckert et al.   Latin American Journal of Aquatic Research

Tilapia lake virus (TiLV) from Peru is genetically close to the Israeli isolates

(2019) Liseth Lucero Huamancha Pulido et al.   AQUACULTURE

Coinfection of tilapia lake virus and Aeromonas hydrophila synergistically increased mortality and worsened the disease severity in tilapia (Oreochromis spp.)

(2019) Pamela Nicholson et al.   AQUACULTURE

Blood and liver biopsy for the non‐destructive screening of tilapia lake virus

(2019) Chutipong Chiamkunakorn et al.   JOURNAL OF FISH DISEASES

Non-lethal sampling for Tilapia Lake Virus detection by RT-qPCR and cell culture

(2018) Pavarit Liamnimitr et al.   AQUACULTURE

Emergence of Tilapia Lake Virus associated with mortalities of farmed Nile Tilapia Oreochromis niloticus (Linnaeus 1758) in India

(2018) B.K. Behera et al.   AQUACULTURE

Real-time reverse transcription recombinase polymerase amplification assay for rapid detection of porcine epidemic diarrhea virus

(2018) Jianchang Wang et al.   JOURNAL OF VIROLOGICAL METHODS

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

Tilapia lake virus: a threat to the global tilapia industry?

(2018) Mona Dverdal Jansen et al.   Reviews in Aquaculture

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

A TaqMan RT-qPCR assay for tilapia lake virus (TiLV) detection in tilapia

(2018) Pitchaporn Waiyamitra et al.   AQUACULTURE

Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity

(2018) Stefano Stella et al.   CELL

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

Saprolegniosis in Nile Tilapia: Identification, Molecular Characterization, and Phylogenetic Analysis of Two Novel Pathogenic Saprolegnia Strains

(2017) Eman Zahran et al.   JOURNAL OF AQUATIC ANIMAL HEALTH

Development and validation of a reverse transcription quantitative polymerase chain reaction for tilapia lake virus detection in clinical samples and experimentally challenged fish

(2017) P Tattiyapong et al.   JOURNAL OF FISH DISEASES

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Establishment of an accurate and fast detection method using molecular beacons in loop-mediated isothermal amplification assay

(2017) Wei Liu et al.   Scientific Reports

Nucleic acid purification from plants, animals and microbes in under 30 seconds

(2017) Yiping Zou et al.   PLOS BIOLOGY

Trichodiniasis in Nile tilapia hatcheries: Diagnosis, parasite:host-stage relationship and treatment

(2016) G.M.R. Valladão et al.   AQUACULTURE

Detection of Tilapia Lake Virus in Clinical Samples by Culturing and Nested Reverse Transcription-PCR

(2016) Japhette Esther Kembou Tsofack et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Characterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia

(2016) Eran Bacharach et al.   mBio

Identification of a Novel RNA Virus Lethal to Tilapia

(2014) M. Eyngor et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis

(2014) Sebastian Kersting et al.   MALARIA JOURNAL

Rapid Detection of Shrimp White Spot Syndrome Virus by Real Time, Isothermal Recombinase Polymerase Amplification Assay

(2014) Xiaoming Xia et al.   PLoS One

Equipment-Free Incubation of Recombinase Polymerase Amplification Reactions Using Body Heat

(2014) Zachary Austin Crannell et al.   PLoS One

Rapid Molecular Assays for the Detection of Yellow Fever Virus in Low-Resource Settings

(2014) Camille Escadafal et al.   PLoS Neglected Tropical Diseases

A predicted hairpin cluster correlates with barriers to PCR, sequencing and possibly BAC recombineering

(2011) Brian L. Nelms et al.   Scientific Reports

The Vienna RNA Websuite

(2008) A. R. Gruber et al.   NUCLEIC ACIDS RESEARCH