Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 61, Issue 47, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202212752
Keywords
DNA Intercalator; Microrna; Photoelectrochemistry; Single Cell Analysis; Zero-Background
Categories
Funding
- National Natural Science Foundation of China [22034003, 21974059, 21327902]
- Excellent Research Program of Nanjing University [ZYJH004]
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This study explores a new technological direction in single-cell analysis by utilizing the rich interplay between light and bio-matter. A PEC nanoreactor is designed for single-cell sampling and detection of intracellular microRNA. The use of light stimulation enables zero-background Faradaic detection of non-electrogenic miR at the single-cell level, revealing differences in miR concentration between the near-nucleus region and the main cytosol.
Rational utilization of the rich light-bio-matter interplay taking place in single-cell analysis represents a new technological direction in the field. The light-fueled operation is expected to achieve advanced photoelectrochemical (PEC) single-cell analysis with unknown possibilities. Here, a PEC nanoreactor capable of single-cell sampling and near zero-background Faradaic detection of intracellular microRNA (miR) is devised by the construction of a small reaction chamber accommodating the target-triggered hybridization chain reaction for binding the metallointercalator of [Ru(bpy)(2)(dppz)](2+) as the signal reporter. Light stimulation of the dsDNA/metallointercalator adduct will induce the generation of photocurrents, underpinning a zero-biased and near zero-background PEC method toward Faradaic detection of non-electrogenic miR at the single-cell level. Using this nanotool, lower miR concentration in the near-nucleus region than that in the main cytosol was revealed.
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