Journal
JOURNAL OF GENETICS AND GENOMICS
Volume 43, Issue 4, Pages 199-207Publisher
SCIENCE PRESS
DOI: 10.1016/j.jgg.2016.02.005
Keywords
Co-expression; Dual-transgene vector; pDT1; pDT7; pDT7G
Funding
- National Natural Science Foundation of China [31171176]
- Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province [20134486]
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In this study, we constructed dual-transgene vectors (pDT1, pDT7, and pDT7G) that simultaneously co-expressed two genes in plants. ACTIN2 and UBQ10 promoters were used to control the expression of these two genes. The 4 x Myc, 3 x HA, and 3 x Flag reporter genes allowed for the convenient identification of a tunable co-expression system in plants, whereas the dexamethasone (Dex) inducible reporter gene C-terminus of the glucocorticoid receptor (cGR) provided Dex-dependent translocation of the fusion gene between the nucleus and cytoplasm. The function of pDT vectors was validated using four pairwise genes in Nicotiana benthamiana or Arabidopsis thaliana. The co-expression efficiency of two genes from the pDT1 and pDT7G vectors was 35% and 42%, respectively, which ensured the generation of sufficient transgenic materials. These pDT vectors are simple, reliable, efficient, and time-saving tools for the co-expression of two genes through a single transformation event and can be used in the study of protein-protein interactions or multi-component complexes.
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