Journal
JOURNAL OF GENERAL VIROLOGY
Volume 97, Issue -, Pages 934-940Publisher
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.000425
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Funding
- European Union grant FLUNIVAC [602604]
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Activation of CD8 T+-cells is an essential part of immune responses elicited by recombinant modified vaccinia virus Ankara (MVA). Strategies to enhance T-cell responses to antigens may be particularly necessary for broadly protective immunization against influenza A virus infections or for candidate vaccines targeting chronic infections and cancer. Here, we tested recombinant MVAs that targeted a model antigen, GFP, to different localizations in infected cells. In vitro characterization demonstrated that GFP accumulated in the nucleus (MVA-nls-GFP), associated with cellular membranes (MVA-myr-GFP) or was equally distributed throughout the cell (MVA-GFP). On vaccination, we found significantly higher levels of GFP-specific CD8 (+)ZT-cells in MVA-myr-GFP-vaccinated BALB/c mice than in those immunized with MVA-GFP or MVA-nls-GFP. Thus, myristoyl modification may be a useful strategy to enhance CD8 T+-cell responses to MVA-delivered target antigens.
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